Restriction map for
pF1KB4593
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsiEI BsiWI BsmAI
BsmBI Bsp1407I BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII Cfr10I CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoT22I
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 114 GTCTAC GT^[AC][GT]AC
AclI 465 AACGTT AA^CGTT
AcyI 286 GGCGTC G[GA]^CG[CT]C
AflIII 1596 ACATGT A^C[GA][CT]GT
AocI 18 CCTGAGG CC^T[ACGT]AGG
Aor51HI 431 AGCGCT AGC^GCT
AspHI 914 GAGCTC G[AT]GC[AT]^C
AvaI 86 CCCGGG C^[CT]CG[GA]G
BalI 589 TGGCCA TGG^CCA
BanI 715 GGTGCC G^G[CT][GA]CC
BanII 914 GAGCTC G[GA]GC[CT]^C
BbsI 454 GAAGAC GAAGAC(2/6)
BglII 1449 AGATCT A^GATCT
BmyI 914 GAGCTC G[AGT]GC[ACT]^C
BpmI 155 CTGGAG CTGGAG(16/14)
BpuAI 454 GAAGAC GAAGAC(2/6)
BsaAI 738 TACGTG [CT]AC^GT[GA]
BsaHI 286 GGCGTC G[GA]^CG[CT]C
BsaWI 1068 ACCGGA [AT]^CCGG[AT]
BsgI 695 GTGCAG GTGCAG(16/14)
BsiHKAI 914 GAGCTC G[AT]GC[AT]^C
BsmI 819 GCATTC GAATGC(1/-1)
BsoBI 86 CCCGGG C^[CT]CG[GA]G
Bsp1286I 914 GAGCTC G[AGT]GC[ACT]^C
BspDI 2291 ATCGAT AT^CGAT
BspHI 1749 TCATGA T^CATGA
BspLU11I 1596 ACATGT A^CATGT
Bsu36I 18 CCTGAGG CC^T[ACGT]AGG
CfoI 432 GCGC GCG^C
ClaI 2291 ATCGAT AT^CGAT
DraI 478 TTTAAA TTT^AAA
EaeI 589 TGGCCA [CT]^GGCC[GA]
Eco47III 431 AGCGCT AGC^GCT
Eco81I 18 CCTGAGG CC^T[ACGT]AGG
EcoRV 1669 GATATC GAT^ATC
HaeII 431 AGCGCT [GA]GCGC^[CT]
HgaI 287 GCGTC GACGC(5/10)
HhaI 432 GCGC GCG^C
Hin1I 286 GGCGTC G[GA]^CG[CT]C
HinP1I 432 GCGC G^CGC
HindIII 639 AAGCTT A^AGCTT
MfeI 1265 CAATTG C^AATTG
MluNI 589 TGGCCA TGG^CCA
MscI 589 TGGCCA TGG^CCA
MspA1I 2180 CAGCGG C[AC]G^C[GT]G
MunI 1265 CAATTG C^AATTG
Psp1406I 465 AACGTT AA^CGTT
RcaI 1749 TCATGA T^CATGA
SacI 914 GAGCTC GAGCT^C
ScaI 1906 AGTACT AGT^ACT
SfaNI 1042 GATGC GCATC(5/9)
SmaI 86 CCCGGG CCC^GGG
SmlI 317 CTCAAG C^T[CT][GA]AG
SpeI 1903 ACTAGT A^CTAGT
SspI 1867 AATATT AAT^ATT
TaqI 2292 TCGA T^CGA
TthHB8I 2292 TCGA T^CGA
XcmI 2323 CCACCTTCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 86 CCCGGG C^CCGGG
XmaI 86 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 829 GTAC GT^AC
1907 GTAC
Asp700I 667 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
850 GAACTTGTTC
BbvI 1062 GCAGC GCAGC(8/12)
1897 GCTGC
BcnI 86 CCCGG CC^[GC]GG
87 CCGGG
BsrDI 688 CATTGC GCAATG(2/0)
1947 CATTGC
BstNI 42 CCAGG CC^[AT]GG
1246 CCAGG
EarI 1768 CTCTTC CTCTTC(1/4)
51 GAAGAG
EcoRI 1476 GAATTC G^AATTC
1545 GAATTC
EcoRII 42 CCAGG ^CC[AT]GG
1246 CCAGG
EcoT14I 1012 CCAAGG C^C[AT][AT]GG
1561 CCAAGG
FauI 1019 CCCGC CCCGC(4/6)
2182 GCGGG
Fnu4HI 1062 GCAGC GC^[ACGT]GC
1897 GCTGC
HapII 87 CCGG C^CGG
1069 CCGG
HpaII 87 CCGG C^CGG
1069 CCGG
ItaI 1062 GCAGC GC^[ACGT]GC
1897 GCTGC
Ksp632I 1768 CTCTTC CTCTTC(1/4)
51 GAAGAG
MspI 87 CCGG C^CGG
1069 CCGG
MvaI 42 CCAGG CC^[AT]GG
1246 CCAGG
NciI 86 CCCGG CC^[GC]GG
87 CCGGG
NdeI 305 CATATG CA^TATG
1180 CATATG
NspI 1377 ACATGC [GA]CATG^[CT]
1596 ACATGT
RsaI 829 GTAC GT^AC
1907 GTAC
StyI 1012 CCAAGG C^C[AT][AT]GG
1561 CCAAGG
TseI 1062 GCAGC G^C[AT]GC
1897 GCTGC
XmnI 667 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
850 GAACTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AvaII BclI BfaI BsaJI
BseRI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I FbaI
FokI HaeIII HinfI HphI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MwoI NdeII
NlaIII NlaIV PleI PpuMI PstI
Sau3AI Sau96I ScrFI SfcI TfiI
Tru9I Tsp45I Tsp509I TspRI XhoII