Restriction map for
pF1KB4640
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BglI BlnI BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeII MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI SwaI
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 942 GCGG CCGC(-3/-1)
AcsI 482 AAATTC [GA]^AATT[CT]
AflII 388 CTTAAG C^TTAAG
AlwNI 723 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 526 GGGCCC GGGCC^C
ApoI 482 AAATTC [GA]^AATT[CT]
Asp700I 3 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 490 GAGCAC G[AT]GC[AT]^C
AspI 1612 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1644 CCTCAGC CCTCAGC(-5/-2)
BclI 882 TGATCA T^GATCA
BcnI 105 CCGGG CC^[GC]GG
BfrI 388 CTTAAG C^TTAAG
BglII 336 AGATCT A^GATCT
BlpI 265 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 265 GCTCAGC GC^T[ACGT]AGC
BsaI 464 GGTCTC GGTCTC(1/5)
BsaWI 580 TCCGGT [AT]^CCGG[AT]
BsiHKAI 490 GAGCAC G[AT]GC[AT]^C
BspMI 627 ACCTGC ACCTGC(4/8)
BsrDI 1426 GCAATG GCAATG(2/0)
BstBI 1682 TTCGAA TT^CGAA
BstYI 336 AGATCT [GA]^GATC[CT]
CelII 265 GCTCAGC GC^T[ACGT]AGC
CfoI 441 GCGC GCG^C
DraI 1567 TTTAAA TTT^AAA
EarI 540 GAAGAG CTCTTC(1/4)
FbaI 882 TGATCA T^GATCA
HhaI 441 GCGC GCG^C
HinP1I 441 GCGC G^CGC
HindIII 1233 AAGCTT A^AGCTT
Ksp632I 540 GAAGAG CTCTTC(1/4)
MfeI 297 CAATTG C^AATTG
MflI 336 AGATCT [GA]^GATC[CT]
MslI 846 CATTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1607 CAGCTG C[AC]G^C[GT]G
MunI 297 CAATTG C^AATTG
NciI 105 CCGGG CC^[GC]GG
NspV 1682 TTCGAA TT^CGAA
PflFI 1612 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1763 CTGCAG CTGCA^G
PvuII 1607 CAGCTG CAG^CTG
SfuI 1682 TTCGAA TT^CGAA
SpeI 832 ACTAGT A^CTAGT
SspI 520 AATATT AAT^ATT
Tth111I 1612 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1438 TCTAGA T^CTAGA
XhoII 336 AGATCT [GA]^GATC[CT]
XmnI 3 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BanII 526 GGGCCC G[GA]GC[CT]^C
1222 GAGCCC
BbsI 1543 GAAGAC GAAGAC(2/6)
1666 GAAGAC
BpuAI 1543 GAAGAC GAAGAC(2/6)
1666 GAAGAC
BsgI 1227 CTGCAC GTGCAG(16/14)
1209 GTGCAG
BstF5I 578 CATCC GGATG(2/0)
1322 GGATG
BstXI 845 CCATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1064 CCAAAGGTGTGG
FokI 578 CATCC GGATG(9/13)
1322 GGATG
HapII 105 CCGG C^CGG
581 CCGG
HpaII 105 CCGG C^CGG
581 CCGG
MspI 105 CCGG C^CGG
581 CCGG
PpuMI 1184 AGGACCC [GA]G^G[AT]CC[CT]
1616 GGGTCCT
SmlI 388 CTTAAG C^T[CT][GA]AG
784 CTCAAG
TaqI 1683 TCGA T^CGA
1824 TCGA
Tsp45I 173 GTCAC ^GT[GC]AC
916 GTGAC
TspRI 633 CAGTG CA[GC]TG[ACGT][ACGT]^
972 CAGTG
TthHB8I 1683 TCGA T^CGA
1824 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AfaI AluI AvaII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp509I XcmI