Restriction map for
pF1KB4641
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI BamHI BbvCI BclI BcnI
BfrI BglI BlpI Bpu1102I BsiEI
BsiWI Bsp1407I BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI FbaI
FseI KpnI MluI NaeI NciI
NcoI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RcaI RsrII SacI
SalI SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 2442 TCCGGA T^CCGGA
AclI 1481 AACGTT AA^CGTT
AlwNI 1998 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1744 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1988 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 58 CCCGAG C^[CT]CG[GA]G
AviII 614 TGCGCA TGC^GCA
AvrII 2766 CCTAGG C^CTAGG
BbeI 1607 GGCGCC GGCGC^C
BbrPI 1069 CACGTG CAC^GTG
BcgI 2589 GCAAGTTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2013 GGATAC GTATCC(6/5)
BglII 1164 AGATCT A^GATCT
BlnI 2766 CCTAGG C^CTAGG
BsaBI 2518 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 85 GAGACC GGTCTC(1/5)
BsaWI 2442 TCCGGA [AT]^CCGG[AT]
BseAI 2442 TCCGGA T^CCGGA
BsgI 129 GTGCAG GTGCAG(16/14)
BsoBI 58 CCCGAG C^[CT]CG[GA]G
BspDI 223 ATCGAT AT^CGAT
BspEI 2442 TCCGGA T^CCGGA
BssSI 2259 CACGAG CACGAG(-5/-1)
BstEII 1433 GGTTACC G^GT[ACGT]ACC
BstPI 1433 GGTTACC G^GT[ACGT]ACC
ClaI 223 ATCGAT AT^CGAT
DraI 2881 TTTAAA TTT^AAA
DraIII 1760 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1458 CCTGTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1433 GGTTACC G^GT[ACGT]ACC
EcoRV 2556 GATATC GAT^ATC
FauI 6 CCCGC CCCGC(4/6)
FspI 614 TGCGCA TGC^GCA
HapII 2443 CCGG C^CGG
HindIII 691 AAGCTT A^AGCTT
HpaI 744 GTTAAC GTT^AAC
HpaII 2443 CCGG C^CGG
KasI 1607 GGCGCC G^GCGCC
KspI 7 CCGCGG CCGC^GG
MamI 2518 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2442 TCCGGA T^CCGGA
MslI 985 CATAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 2443 CCGG C^CGG
NarI 1607 GGCGCC GG^CGCC
NdeI 2196 CATATG CA^TATG
NheI 1614 GCTAGC G^CTAGC
PflFI 1988 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 685 GAGTC GAGTC(4/5)
PmaCI 1069 CACGTG CAC^GTG
PmlI 1069 CACGTG CAC^GTG
Psp1406I 1481 AACGTT AA^CGTT
SacII 7 CCGCGG CCGC^GG
SfoI 1607 GGCGCC GGC^GCC
SphI 2691 GCATGC GCATG^C
SspI 619 AATATT AAT^ATT
Tsp45I 374 GTGAC ^GT[GC]AC
Tth111I 1988 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1744 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 8 CGCG CG^CG
121 CGCG
AcyI 1607 GGCGCC G[GA]^CG[CT]C
2779 GGCGTC
AflIII 1001 ACGTGT A^C[GA][CT]GT
1192 ACACGT
AspHI 76 GAGCAC G[AT]GC[AT]^C
2117 GTGCTC
AvaII 995 GGTCC G^G[AT]CC
2364 GGACC
BalI 1053 TGGCCA TGG^CCA
2005 TGGCCA
BanI 1607 GGCGCC G^G[CT][GA]CC
2770 GGCACC
BanII 109 GAGCCC G[GA]GC[CT]^C
2311 GAGCCC
BbsI 1114 GAAGAC GAAGAC(2/6)
2894 GTCTTC
BpuAI 1114 GAAGAC GAAGAC(2/6)
2894 GTCTTC
BsaHI 1607 GGCGCC G[GA]^CG[CT]C
2779 GGCGTC
BsiHKAI 76 GAGCAC G[AT]GC[AT]^C
2117 GTGCTC
BsmBI 46 GAGACG CGTCTC(1/5)
389 GAGACG
BsmFI 2363 GGGAC GGGAC(10/14)
2129 GTCCC
BspMI 1437 ACCTGC ACCTGC(4/8)
2366 ACCTGC
BstAPI 882 GCAGCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2827 GCAGATTATGC
BstUI 8 CGCG CG^CG
121 CGCG
DsaI 7 CCGCGG C^C[GA][CT]GG
2133 CCACGG
EaeI 1053 TGGCCA [CT]^GGCC[GA]
2005 TGGCCA
HaeII 35 GGCGCT [GA]GCGC^[CT]
1607 GGCGCC
HgaI 1497 GCGTC GACGC(5/10)
2780 GCGTC
Hin1I 1607 GGCGCC G[GA]^CG[CT]C
2779 GGCGTC
HincII 298 GTCAAC GT[CT]^[GA]AC
744 GTTAAC
HindII 298 GTCAAC GT[CT]^[GA]AC
744 GTTAAC
MfeI 2729 CAATTG C^AATTG
2822 CAATTG
MluNI 1053 TGGCCA TGG^CCA
2005 TGGCCA
MscI 1053 TGGCCA TGG^CCA
2005 TGGCCA
MunI 2729 CAATTG C^AATTG
2822 CAATTG
MvnI 8 CGCG CG^CG
121 CGCG
NspI 380 ACATGC [GA]CATG^[CT]
2691 GCATGC
PflMI 1081 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2204 CCAGTGAGTGG
PpuMI 994 GGGTCCT [GA]G^G[AT]CC[CT]
2363 GGGACCT
PstI 13 CTGCAG CTGCA^G
1825 CTGCAG
PvuII 16 CAGCTG CAG^CTG
1627 CAGCTG
ScaI 446 AGTACT AGT^ACT
1832 AGTACT
SfcI 13 CTGCAG C^T[GA][CT]AG
1825 CTGCAG
Van91I 1081 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2204 CCAGTGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BmyI BpmI
BsaAI BsaJI BseRI BsiYI BslI
BsmAI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NsiI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII