Restriction map for
pF1KB4658
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BbeI BbvCI BciVI BfrI BglII
BlnI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI HindIII KasI KpnI
MluI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmeI PshBI PvuI RcaI
RsrII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Van91I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1726 GACGTC GACGT^C
AccI 2359 GTCTAC GT^[AC][GT]AC
AccIII 1796 TCCGGA T^CCGGA
AclI 1846 AACGTT AA^CGTT
AcsI 246 GAATTT [GA]^AATT[CT]
ApoI 246 GAATTT [GA]^AATT[CT]
AviII 977 TGCGCA TGC^GCA
BanI 1549 GGCACC G^G[CT][GA]CC
BbrPI 964 CACGTG CAC^GTG
BcgI 980 GCAACCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 964 CACGTG [CT]AC^GT[GA]
BsaBI 807 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1796 TCCGGA [AT]^CCGG[AT]
BseAI 1796 TCCGGA T^CCGGA
BspEI 1796 TCCGGA T^CCGGA
BspMI 1436 ACCTGC ACCTGC(4/8)
BsrDI 725 GCAATG GCAATG(2/0)
BstXI 2171 CCAAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 194 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2082 GACAGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 636 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 977 TGCGCA TGC^GCA
HpaI 1849 GTTAAC GTT^AAC
MamI 807 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 432 CAATTG C^AATTG
MroI 1796 TCCGGA T^CCGGA
MseI 1850 TTAA T^TAA
MunI 432 CAATTG C^AATTG
NaeI 1776 GCCGGC GCC^GGC
NcoI 1598 CCATGG C^CATGG
NgoMIV 1776 GCCGGC G^CCGGC
PaeR7I 99 CTCGAG C^TCGAG
PmaCI 964 CACGTG CAC^GTG
PmlI 964 CACGTG CAC^GTG
Psp1406I 1846 AACGTT AA^CGTT
SexAI 641 ACCAGGT A^CC[AT]GGT
SmlI 99 CTCGAG C^T[CT][GA]AG
SphI 902 GCATGC GCATG^C
Tru9I 1850 TTAA T^TAA
XcmI 353 CCAGCTGGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 99 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AocI 295 CCTCAGG CC^T[ACGT]AGG
1854 CCTCAGG
AspI 166 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1758 GACACTGTC
AvaI 99 CTCGAG C^[CT]CG[GA]G
1509 CTCGGG
BamHI 389 GGATCC G^GATCC
2208 GGATCC
BbsI 565 GTCTTC GAAGAC(2/6)
2122 GTCTTC
BclI 809 TGATCA T^GATCA
1094 TGATCA
BglI 367 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
749 GCCCCAACGGC
BlpI 1315 GCTCAGC GC^T[ACGT]AGC
1867 GCTCAGC
Bpu1102I 1315 GCTCAGC GC^T[ACGT]AGC
1867 GCTCAGC
BpuAI 565 GTCTTC GAAGAC(2/6)
2122 GTCTTC
BsaI 559 GAGACC GGTCTC(1/5)
2350 GAGACC
BsoBI 99 CTCGAG C^[CT]CG[GA]G
1509 CTCGGG
BsrFI 1769 ACCGGC [GA]^CCGG[CT]
1776 GCCGGC
Bsu36I 295 CCTCAGG CC^T[ACGT]AGG
1854 CCTCAGG
CelII 1315 GCTCAGC GC^T[ACGT]AGC
1867 GCTCAGC
Cfr10I 1769 ACCGGC [GA]^CCGG[CT]
1776 GCCGGC
EarI 211 CTCTTC CTCTTC(1/4)
1792 CTCTTC
Eco81I 295 CCTCAGG CC^T[ACGT]AGG
1854 CCTCAGG
FbaI 809 TGATCA T^GATCA
1094 TGATCA
HaeII 1284 AGCGCC [GA]GCGC^[CT]
1943 GGCGCT
Ksp632I 211 CTCTTC CTCTTC(1/4)
1792 CTCTTC
PflFI 166 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1758 GACACTGTC
PshAI 1722 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2352 GACCGAGGTC
SacI 288 GAGCTC GAGCT^C
1313 GAGCTC
ScaI 518 AGTACT AGT^ACT
2267 AGTACT
TfiI 132 GATTC G^A[AT]TC
203 GATTC
Tth111I 166 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1758 GACACTGTC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaII
BalI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII