Restriction map for
pF1KB4670
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspHI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HindIII HpaI
KasI KpnI MaeI MfeI MluI
MluNI MscI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RsrII SacI SalI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Van91I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 334 GACGTC GACGT^C
AccIII 807 TCCGGA T^CCGGA
ApaI 700 GGGCCC GGGCC^C
AseI 982 ATTAAT AT^TAAT
AsnI 982 ATTAAT AT^TAAT
AspI 556 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 308 CCCGGG C^[CT]CG[GA]G
BanII 700 GGGCCC G[GA]GC[CT]^C
BbsI 373 GTCTTC GAAGAC(2/6)
BpuAI 373 GTCTTC GAAGAC(2/6)
BsaBI 799 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 807 TCCGGA [AT]^CCGG[AT]
BseAI 807 TCCGGA T^CCGGA
BsgI 300 CTGCAC GTGCAG(16/14)
BsiEI 91 CGGCCG CG[GA][CT]^CG
BsmFI 224 GGGAC GGGAC(10/14)
BsoBI 308 CCCGGG C^[CT]CG[GA]G
BspEI 807 TCCGGA T^CCGGA
BsrDI 1188 GCAATG GCAATG(2/0)
BsrFI 183 GCCGGC [GA]^CCGG[CT]
BssHII 155 GCGCGC G^CGCGC
Cfr10I 183 GCCGGC [GA]^CCGG[CT]
DrdI 364 GACATCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 91 CGGCCG C^GGCCG
EclXI 91 CGGCCG C^GGCCG
Eco52I 91 CGGCCG C^GGCCG
EcoNI 784 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 205 GTCAAC GT[CT]^[GA]AC
HindII 205 GTCAAC GT[CT]^[GA]AC
HinfI 349 GACTC G^A[ACGT]TC
KspI 88 CCGCGG CCGC^GG
MaeII 335 ACGT A^CGT
MaeIII 794 GTGAC ^GT[ACGT]AC
MamI 799 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 807 TCCGGA T^CCGGA
MseI 983 TTAA T^TAA
NaeI 183 GCCGGC GCC^GGC
NcoI 1094 CCATGG C^CATGG
NgoMIV 183 GCCGGC G^CCGGC
PflFI 556 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 349 GACTC GAGTC(4/5)
PpuMI 1146 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 982 ATTAAT AT^TAAT
PstI 1142 CTGCAG CTGCA^G
SacII 88 CCGCGG CCGC^GG
SbfI 1141 CCTGCAGG CCTGCA^GG
ScaI 456 AGTACT AGT^ACT
SexAI 783 ACCTGGT A^CC[AT]GGT
SfcI 1142 CTGCAG C^T[GA][CT]AG
SmaI 308 CCCGGG CCC^GGG
Sse8387I 1141 CCTGCAGG CCTGCA^GG
Tru9I 983 TTAA T^TAA
Tsp45I 794 GTGAC ^GT[GC]AC
Tth111I 556 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 308 CCCGGG C^CCGGG
XmaI 308 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 211 GACGCC G[GA]^CG[CT]C
334 GACGTC
AvaII 489 GGTCC G^G[AT]CC
1147 GGACC
BamHI 196 GGATCC G^GATCC
1133 GGATCC
BanI 245 GGCACC G^G[CT][GA]CC
691 GGCACC
BmyI 244 GGGCAC G[AGT]GC[ACT]^C
700 GGGCCC
BpmI 705 CTCCAG CTGGAG(16/14)
317 CTGGAG
BsaHI 211 GACGCC G[GA]^CG[CT]C
334 GACGTC
BsaI 74 GAGACC GGTCTC(1/5)
656 GAGACC
BseRI 342 CTCCTC GAGGAG(10/8)
615 CTCCTC
BsmBI 332 GAGACG CGTCTC(1/5)
575 GAGACG
Bsp1286I 244 GGGCAC G[AGT]GC[ACT]^C
700 GGGCCC
BspHI 597 TCATGA T^CATGA
869 TCATGA
BsrBI 130 CCGCTC CCGCTC(-3/-3)
729 GAGCGG
BstYI 196 GGATCC [GA]^GATC[CT]
1133 GGATCC
DdeI 460 CTAAG C^T[ACGT]AG
684 CTCAG
EaeI 91 CGGCCG [CT]^GGCC[GA]
185 CGGCCA
EarI 883 CTCTTC CTCTTC(1/4)
916 GAAGAG
EcoT14I 1062 CCAAGG C^C[AT][AT]GG
1094 CCATGG
HgaI 211 GACGC GACGC(5/10)
232 GACGC
Hin1I 211 GACGCC G[GA]^CG[CT]C
334 GACGTC
Ksp632I 883 CTCTTC CTCTTC(1/4)
916 GAAGAG
MflI 196 GGATCC [GA]^GATC[CT]
1133 GGATCC
MspA1I 88 CCGCGG C[AC]G^C[GT]G
200 CCGCTG
RcaI 597 TCATGA T^CATGA
869 TCATGA
SapI 916 GAAGAGC GCTCTTC(1/4)
882 GCTCTTC
SfaNI 667 GATGC GCATC(5/9)
891 GCATC
StyI 1062 CCAAGG C^C[AT][AT]GG
1094 CCATGG
TaqI 554 TCGA T^CGA
1029 TCGA
Tsp509I 433 AATT ^AATT
957 AATT
TthHB8I 554 TCGA T^CGA
1029 TCGA
XhoII 196 GGATCC [GA]^GATC[CT]
1133 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BcnI BsaJI BsiYI BslI
BsmAI BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DpnII DraII DsaI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboI MboII MnlI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TseI TspRI