Restriction map for
pF1KB4679
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BbvI
BclI BcnI BfaI BfrI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HapII Hin1I HincII
HindII HindIII HpaI HpaII KasI
Ksp632I MaeI MamI MfeI MluI
MluNI MroI MscI MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NsiI PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TseI Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 242 GGTACC G^GTACC
AluI 318 AGCT AG^CT
AlwI 414 GGATC GGATC(4/5)
Asp718I 242 GGTACC G^GTACC
AspHI 317 GAGCTC G[AT]GC[AT]^C
AvaI 323 CCCGAG C^[CT]CG[GA]G
BanI 242 GGTACC G^G[CT][GA]CC
BanII 317 GAGCTC G[GA]GC[CT]^C
BbsI 202 GAAGAC GAAGAC(2/6)
BcgI 467 GCACTGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 590 GGATAC GTATCC(6/5)
BglI 287 GCCGAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 541 AGATCT A^GATCT
BmyI 317 GAGCTC G[AGT]GC[ACT]^C
BpmI 71 CTCCAG CTGGAG(16/14)
BpuAI 202 GAAGAC GAAGAC(2/6)
BsiEI 273 CGGCCG CG[GA][CT]^CG
BsiHKAI 317 GAGCTC G[AT]GC[AT]^C
BsmBI 194 GAGACG CGTCTC(1/5)
BsmFI 156 GGGAC GGGAC(10/14)
BsoBI 323 CCCGAG C^[CT]CG[GA]G
Bsp1286I 317 GAGCTC G[AGT]GC[ACT]^C
BspHI 546 TCATGA T^CATGA
BspMI 430 GCAGGT ACCTGC(4/8)
BssKI 347 CCAGG ^CC[ACGT]GG
BstBI 515 TTCGAA TT^CGAA
BstNI 347 CCAGG CC^[AT]GG
BstYI 541 AGATCT [GA]^GATC[CT]
CfoI 119 GCGC GCG^C
DdeI 533 CTAAG C^T[ACGT]AG
DraI 408 TTTAAA TTT^AAA
DraII 185 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 273 CGGCCG [CT]^GGCC[GA]
EagI 273 CGGCCG C^GGCCG
EclXI 273 CGGCCG C^GGCCG
Eco52I 273 CGGCCG C^GGCCG
EcoO109I 185 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 347 CCAGG ^CC[AT]GG
EcoT14I 86 CCAAGG C^C[AT][AT]GG
HaeII 118 GGCGCT [GA]GCGC^[CT]
HhaI 119 GCGC GCG^C
HinP1I 119 GCGC G^CGC
HphI 433 GGTGA GGTGA(8/7)
KpnI 242 GGTACC GGTAC^C
KspI 150 CCGCGG CCGC^GG
MaeIII 62 GTAAC ^GT[ACGT]AC
MflI 541 AGATCT [GA]^GATC[CT]
MseI 409 TTAA T^TAA
MspA1I 150 CCGCGG C[AC]G^C[GT]G
MvaI 347 CCAGG CC^[AT]GG
NruI 219 TCGCGA TCG^CGA
NspI 419 ACATGC [GA]CATG^[CT]
NspV 515 TTCGAA TT^CGAA
PpuMI 185 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 546 TCATGA T^CATGA
SacI 317 GAGCTC GAGCT^C
SacII 150 CCGCGG CCGC^GG
ScrFI 347 CCAGG CC^[ACGT]GG
SfaNI 50 GCATC GCATC(5/9)
SfuI 515 TTCGAA TT^CGAA
SmlI 139 CTCAAG C^T[CT][GA]AG
StyI 86 CCAAGG C^C[AT][AT]GG
Tru9I 409 TTAA T^TAA
Tsp509I 586 AATT ^AATT
XcmI 388 CCAAGTGGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 541 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 243 GTAC GT^AC
487 GTAC
AflIII 101 ACGTGT A^C[GA][CT]GT
562 ACACGT
AvaII 157 GGACC G^G[AT]CC
186 GGACC
BsaAI 55 TACGTG [CT]AC^GT[GA]
563 CACGTA
BsmAI 194 GAGAC GTCTC(1/5)
373 GTCTC
BsrI 469 ACTGG ACTGG(1/-1)
522 ACTGG
BstF5I 78 CATCC GGATG(2/0)
344 CATCC
DpnII 415 GATC ^GATC
542 GATC
DsaI 150 CCGCGG C^C[GA][CT]GG
276 CCGTGG
FokI 78 CATCC GGATG(9/13)
344 CATCC
HgaI 196 GACGC GACGC(5/10)
205 GACGC
HinfI 18 GATTC G^A[ACGT]TC
144 GAATC
MboI 415 GATC ^GATC
542 GATC
MboII 202 GAAGA GAAGA(8/7)
496 GAAGA
MslI 51 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
418 CACATGCGTG
NdeII 415 GATC ^GATC
542 GATC
NlaIII 420 CATG CATG^
547 CATG
RsaI 243 GTAC GT^AC
487 GTAC
Sau3AI 415 GATC ^GATC
542 GATC
TfiI 18 GATTC G^A[AT]TC
144 GAATC
TspRI 468 CACTG CA[GC]TG[ACGT][ACGT]^
595 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AccII AciI BsaJI BsiYI BslI
BstUI Cfr13I FauI Fnu4HI HaeIII
ItaI MaeII MnlI MvnI MwoI
NlaIV Sau96I TaqI TthHB8I