Restriction map for
pF1KB4680
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BalI BamHI BanI
BbeI BbvCI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiWI BsmI BspDI BspEI BspHI
BsrBI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI Eam1105I Eco47III
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tru9I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 20 GTAGAC GT^[AC][GT]AC
AcsI 238 GAATTC [GA]^AATT[CT]
Alw44I 1375 GTGCAC G^TGCAC
AlwI 1158 GATCC GGATC(4/5)
AocI 948 CCTGAGG CC^T[ACGT]AGG
ApaLI 1375 GTGCAC G^TGCAC
ApoI 238 GAATTC [GA]^AATT[CT]
Asp700I 1248 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1296 CTCGGG C^[CT]CG[GA]G
BanII 1089 GAGCCC G[GA]GC[CT]^C
BbrPI 1230 CACGTG CAC^GTG
BfaI 292 CTAG C^TAG
BglII 1103 AGATCT A^GATCT
BpmI 259 CTCCAG CTGGAG(16/14)
BsaAI 1230 CACGTG [CT]AC^GT[GA]
BsiEI 530 CGGCCG CG[GA][CT]^CG
BsmBI 1074 CGTCTC CGTCTC(1/5)
BsoBI 1296 CTCGGG C^[CT]CG[GA]G
BsrFI 930 GCCGGC [GA]^CCGG[CT]
BssSI 1282 CACGAG CACGAG(-5/-1)
Bsu36I 948 CCTGAGG CC^T[ACGT]AGG
Cfr10I 930 GCCGGC [GA]^CCGG[CT]
DraII 1436 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1320 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 721 GACCTGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 530 CGGCCG [CT]^GGCC[GA]
EagI 530 CGGCCG C^GGCCG
EarI 777 CTCTTC CTCTTC(1/4)
EclXI 530 CGGCCG C^GGCCG
Eco52I 530 CGGCCG C^GGCCG
Eco81I 948 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1436 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 238 GAATTC G^AATTC
HaeII 1277 AGCGCC [GA]GCGC^[CT]
Ksp632I 777 CTCTTC CTCTTC(1/4)
KspI 1094 CCGCGG CCGC^GG
MaeI 292 CTAG C^TAG
NaeI 930 GCCGGC GCC^GGC
NgoMIV 930 GCCGGC G^CCGGC
NheI 291 GCTAGC G^CTAGC
PflMI 1365 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1230 CACGTG CAC^GTG
PmlI 1230 CACGTG CAC^GTG
PpuMI 1436 GGGACCT [GA]G^G[AT]CC[CT]
SacII 1094 CCGCGG CCGC^GG
ScaI 1343 AGTACT AGT^ACT
SexAI 803 ACCTGGT A^CC[AT]GGT
TaqI 422 TCGA T^CGA
TfiI 694 GATTC G^A[AT]TC
TthHB8I 422 TCGA T^CGA
Van91I 1365 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1248 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 314 ACATGT A^C[GA][CT]GT
545 ACATGT
BcnI 981 CCGGG CC^[GC]GG
1433 CCGGG
BglI 934 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1091 GCCCCGCGGGC
BsgI 75 CTGCAC GTGCAG(16/14)
808 GTGCAG
Bsp1407I 1265 TGTACA T^GTACA
1442 TGTACA
BspLU11I 314 ACATGT A^CATGT
545 ACATGT
BspMI 810 GCAGGT ACCTGC(4/8)
1418 GCAGGT
BsrGI 1265 TGTACA T^GTACA
1442 TGTACA
BstAPI 1319 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1403 GCAAGTGGTGC
BstYI 1103 AGATCT [GA]^GATC[CT]
1157 AGATCC
DpnII 1104 GATC ^GATC
1158 GATC
HincII 706 GTTGAC GT[CT]^[GA]AC
1219 GTCAAC
HindII 706 GTTGAC GT[CT]^[GA]AC
1219 GTCAAC
MboI 1104 GATC ^GATC
1158 GATC
MflI 1103 AGATCT [GA]^GATC[CT]
1157 AGATCC
NciI 981 CCGGG CC^[GC]GG
1433 CCGGG
NdeII 1104 GATC ^GATC
1158 GATC
NlaIV 942 GGCTCC GG[ACGT]^[ACGT]CC
1436 GGGACC
PstI 645 CTGCAG CTGCA^G
1153 CTGCAG
Sau3AI 1104 GATC ^GATC
1158 GATC
SspBI 1265 TGTACA T^GTACA
1442 TGTACA
Tsp45I 71 GTGAC ^GT[GC]AC
679 GTCAC
Tsp509I 239 AATT ^AATT
497 AATT
XhoII 1103 AGATCT [GA]^GATC[CT]
1157 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AspI AvaII BbsI BbvI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DsaI EcoNI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NlaIII NspI PflFI
PleI PvuII RsaI Sau96I ScrFI
SfaNI SfcI SmlI TseI TspRI
Tth111I