Restriction map for
pF1KB4698
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccII AccIII AflII AgeI
Alw44I AlwNI ApaLI AscI AseI
AsnI Asp700I AspI AviII BalI
BbrPI BbsI BcgI BciVI BfrI
BglI BlpI Bpu1102I BpuAI BsaAI
BsaWI BseAI BsgI BsiWI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EcoO65I FauI FseI FspI
HindIII HpaI KspI MluI MluNI
MroI MscI MspA1I MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 695 GGTACC G^GTACC
AclI 2434 AACGTT AA^CGTT
AflIII 684 ACACGT A^C[GA][CT]GT
AhdI 277 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 939 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1319 AGCGCT AGC^GCT
Asp718I 695 GGTACC G^GTACC
AspEI 277 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1555 CTCGGG C^[CT]CG[GA]G
AvaII 2075 GGACC G^G[AT]CC
AvrII 100 CCTAGG C^CTAGG
BamHI 2278 GGATCC G^GATCC
BbeI 106 GGCGCC GGCGC^C
BbvCI 1969 GCTGAGG CCTCAGC(-5/-2)
BcnI 1148 CCCGG CC^[GC]GG
BlnI 100 CCTAGG C^CTAGG
BsiEI 2120 CGGCCG CG[GA][CT]^CG
BsmBI 324 CGTCTC CGTCTC(1/5)
BsoBI 1555 CTCGGG C^[CT]CG[GA]G
Bst1107I 882 GTATAC GTA^TAC
BstXI 1256 CCACAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 882 GTATAC GTA^TAC
Bsu36I 939 CCTAAGG CC^T[ACGT]AGG
Cac8I 483 GCAAGC GC[ACGT]^[ACGT]GC
EaeI 2120 CGGCCG [CT]^GGCC[GA]
EagI 2120 CGGCCG C^GGCCG
Eam1105I 277 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2120 CGGCCG C^GGCCG
Eco47III 1319 AGCGCT AGC^GCT
Eco52I 2120 CGGCCG C^GGCCG
Eco81I 939 CCTAAGG CC^T[ACGT]AGG
EcoNI 1041 CCTCAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2583 GAATTC G^AATTC
EcoRV 1560 GATATC GAT^ATC
EcoT22I 13 ATGCAT ATGCA^T
HapII 1149 CCGG C^CGG
HgaI 2333 GCGTC GACGC(5/10)
HpaII 1149 CCGG C^CGG
KasI 106 GGCGCC G^GCGCC
KpnI 695 GGTACC GGTAC^C
MslI 375 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1149 CCGG C^CGG
NarI 106 GGCGCC GG^CGCC
NciI 1148 CCCGG CC^[GC]GG
NsiI 13 ATGCAT ATGCA^T
NspI 2081 ACATGC [GA]CATG^[CT]
PflMI 349 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2434 AACGTT AA^CGTT
PstI 1349 CTGCAG CTGCA^G
SexAI 662 ACCAGGT A^CC[AT]GGT
SfoI 106 GGCGCC GGC^GCC
SmlI 1231 CTTGAG C^T[CT][GA]AG
SspI 2560 AATATT AAT^ATT
Van91I 349 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1000 CCAGCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 658 GTAGAC GT^[AC][GT]AC
882 GTATAC
AcyI 106 GGCGCC G[GA]^CG[CT]C
2332 GGCGTC
ApaI 1037 GGGCCC GGGCC^C
1144 GGGCCC
BclI 1571 TGATCA T^GATCA
1892 TGATCA
BfaI 101 CTAG C^TAG
2135 CTAG
BsaBI 1560 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1893 GATCATAATC
BsaHI 106 GGCGCC G[GA]^CG[CT]C
2332 GGCGTC
BsmI 975 GAATGC GAATGC(1/-1)
2550 GAATGC
BstAPI 483 GCAAGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2545 GCAAAGAATGC
FbaI 1571 TGATCA T^GATCA
1892 TGATCA
HaeII 106 GGCGCC [GA]GCGC^[CT]
1319 AGCGCT
Hin1I 106 GGCGCC G[GA]^CG[CT]C
2332 GGCGTC
MaeI 101 CTAG C^TAG
2135 CTAG
MamI 1560 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1893 GATCATAATC
MfeI 1391 CAATTG C^AATTG
1487 CAATTG
MunI 1391 CAATTG C^AATTG
1487 CAATTG
SfcI 1349 CTGCAG C^T[GA][CT]AG
1937 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanI BanII BbvI
BglII BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrDI BsrI
BssKI BstF5I BstNI BstYI CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HincII HindII
HinfI HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII