Restriction map for
pF1KB4699
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MluI MroI MseI MslI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 601 GTCTAC GT^[AC][GT]AC
AfaI 946 GTAC GT^AC
AflIII 409 ACGTGT A^C[GA][CT]GT
AlwI 180 GGATC GGATC(4/5)
AlwNI 965 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 551 CCTTAGG CC^T[ACGT]AGG
ApaI 894 GGGCCC GGGCC^C
AspHI 158 GAGCAC G[AT]GC[AT]^C
AspI 438 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 894 GGGCCC G[GA]GC[CT]^C
BbvCI 1035 GCTGAGG CCTCAGC(-5/-2)
BcnI 465 CCCGG CC^[GC]GG
BglII 205 AGATCT A^GATCT
BpmI 698 CTGGAG CTGGAG(16/14)
BsiEI 630 CGATCG CG[GA][CT]^CG
BsiHKAI 158 GAGCAC G[AT]GC[AT]^C
BsrBI 528 CCGCTC CCGCTC(-3/-3)
BstEII 1082 GGTTACC G^GT[ACGT]ACC
BstPI 1082 GGTTACC G^GT[ACGT]ACC
BstXI 761 CCACCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 551 CCTTAGG CC^T[ACGT]AGG
Cac8I 729 GCTAGC GC[ACGT]^[ACGT]GC
CfoI 1041 GCGC GCG^C
DsaI 452 CCATGG C^C[GA][CT]GG
EarI 334 CTCTTC CTCTTC(1/4)
Eco81I 551 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1082 GGTTACC G^GT[ACGT]ACC
EcoRV 196 GATATC GAT^ATC
HaeII 1040 GGCGCT [GA]GCGC^[CT]
HapII 466 CCGG C^CGG
HhaI 1041 GCGC GCG^C
HinP1I 1041 GCGC G^CGC
HpaII 466 CCGG C^CGG
Ksp632I 334 CTCTTC CTCTTC(1/4)
MfeI 723 CAATTG C^AATTG
MspI 466 CCGG C^CGG
MunI 723 CAATTG C^AATTG
NciI 465 CCCGG CC^[GC]GG
NcoI 452 CCATGG C^CATGG
NheI 729 GCTAGC G^CTAGC
PflFI 438 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 747 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 1068 CTGCAG CTGCA^G
PvuI 630 CGATCG CGAT^CG
RsaI 946 GTAC GT^AC
SapI 333 GCTCTTC GCTCTTC(1/4)
SexAI 902 ACCTGGT A^CC[AT]GGT
SmlI 607 CTCAAG C^T[CT][GA]AG
TaqI 633 TCGA T^CGA
Tsp45I 349 GTCAC ^GT[GC]AC
Tth111I 438 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 633 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 378 CCGC CCGC(-3/-1)
528 CCGC
AvaII 694 GGTCC G^G[AT]CC
748 GGTCC
BalI 449 TGGCCA TGG^CCA
708 TGGCCA
BsmFI 268 GGGAC GGGAC(10/14)
749 GTCCC
BstF5I 579 CATCC GGATG(2/0)
977 GGATG
BstYI 180 GGATCT [GA]^GATC[CT]
205 AGATCT
EaeI 449 TGGCCA [CT]^GGCC[GA]
708 TGGCCA
FokI 579 CATCC GGATG(9/13)
977 GGATG
HphI 350 TCACC GGTGA(8/7)
375 TCACC
MaeII 409 ACGT A^CGT
476 ACGT
MaeIII 349 GTCAC ^GT[ACGT]AC
1083 GTTAC
MflI 180 GGATCT [GA]^GATC[CT]
205 AGATCT
MluNI 449 TGGCCA TGG^CCA
708 TGGCCA
MscI 449 TGGCCA TGG^CCA
708 TGGCCA
MwoI 101 GCGTGGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1043 GCTGTTTCAGC
SfaNI 341 GCATC GCATC(5/9)
773 GCATC
Tsp509I 213 AATT ^AATT
724 AATT
XhoII 180 GGATCT [GA]^GATC[CT]
205 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI BbvI BfaI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI HaeIII
HinfI ItaI MaeI MboI MboII
MnlI MspA1I MvaI NdeII NlaIII
NlaIV PleI PvuII Sau3AI Sau96I
ScrFI SfcI StyI TfiI TseI
TspRI