Restriction map for
pF1KB4700
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
Alw44I ApaI ApaLI AscI AspEI
AviII AvrII BamHI BbeI BbrPI
BbvCI BclI BfaI BfrI BglI
BlnI BsaAI BseAI BsiWI BsmBI
BsmI BspEI BspHI BspMI BsrBI
BssHII BssSI BstBI BstEII BstPI
BstXI DraIII Eam1105I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HpaI
KasI KspI MaeI MluI MroI
NaeI NarI NcoI NgoMIV NheI
NotI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI Psp1406I PvuI
RcaI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SmaI
SnaBI SpeI Sse8387I StuI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 1537 GGTACC G^GTACC
AcyI 60 GGCGTC G[GA]^CG[CT]C
AgeI 2164 ACCGGT A^CCGGT
AocI 829 CCTGAGG CC^T[ACGT]AGG
Aor51HI 18 AGCGCT AGC^GCT
Asp700I 1522 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1537 GGTACC G^GTACC
AspHI 2610 GAGCTC G[AT]GC[AT]^C
AvaI 645 CTCGGG C^[CT]CG[GA]G
BalI 453 TGGCCA TGG^CCA
BbsI 2341 GAAGAC GAAGAC(2/6)
BciVI 2457 GTATCC GTATCC(6/5)
BglII 1775 AGATCT A^GATCT
BlpI 1984 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1984 GCTCAGC GC^T[ACGT]AGC
BpuAI 2341 GAAGAC GAAGAC(2/6)
BsaBI 1219 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 60 GGCGTC G[GA]^CG[CT]C
BsaI 328 GAGACC GGTCTC(1/5)
BsiEI 1100 CGGCCG CG[GA][CT]^CG
BsiHKAI 2610 GAGCTC G[AT]GC[AT]^C
BsoBI 645 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1256 TGTACA T^GTACA
BspDI 1574 ATCGAT AT^CGAT
BsrGI 1256 TGTACA T^GTACA
Bst1107I 1032 GTATAC GTA^TAC
BstAPI 871 GCAATTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1032 GTATAC GTA^TAC
Bsu36I 829 CCTGAGG CC^T[ACGT]AGG
CelII 1984 GCTCAGC GC^T[ACGT]AGC
ClaI 1574 ATCGAT AT^CGAT
CpoI 2462 CGGTCCG CG^G[AT]CCG
DrdI 1321 GACGGGTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1100 CGGCCG C^GGCCG
EclXI 1100 CGGCCG C^GGCCG
Eco47III 18 AGCGCT AGC^GCT
Eco52I 1100 CGGCCG C^GGCCG
Eco81I 829 CCTGAGG CC^T[ACGT]AGG
EcoNI 51 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1412 CCAAGG C^C[AT][AT]GG
HaeII 18 AGCGCT [GA]GCGC^[CT]
HgaI 61 GCGTC GACGC(5/10)
Hin1I 60 GGCGTC G[GA]^CG[CT]C
HindIII 273 AAGCTT A^AGCTT
KpnI 1537 GGTACC GGTAC^C
MamI 1219 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 453 TGGCCA TGG^CCA
MscI 453 TGGCCA TGG^CCA
NruI 678 TCGCGA TCG^CGA
PinAI 2164 ACCGGT A^CCGGT
PshAI 1116 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2462 CGGTCCG CG^G[AT]CCG
SacI 2610 GAGCTC GAGCT^C
SexAI 806 ACCAGGT A^CC[AT]GGT
SgrAI 2163 CACCGGTG C[GA]^CCGG[CT]G
SphI 294 GCATGC GCATG^C
SspBI 1256 TGTACA T^GTACA
StyI 1412 CCAAGG C^C[AT][AT]GG
XmnI 1522 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1032 GTATAC GT^[AC][GT]AC
1186 GTAGAC
AccII 679 CGCG CG^CG
2089 CGCG
AflIII 956 ACATGT A^C[GA][CT]GT
2696 ACATGT
AlwNI 599 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
824 CAGTTCCTG
AspI 478 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
754 GACATTGTC
BanII 2449 GAGCCC G[GA]GC[CT]^C
2610 GAGCTC
BcnI 692 CCCGG CC^[GC]GG
2244 CCGGG
BsaWI 2164 ACCGGT [AT]^CCGG[AT]
2460 TCCGGT
BseRI 385 GAGGAG GAGGAG(10/8)
2333 GAGGAG
BsgI 2124 CTGCAC GTGCAG(16/14)
125 GTGCAG
BspLU11I 956 ACATGT A^CATGT
2696 ACATGT
BsrDI 2175 CATTGC GCAATG(2/0)
188 GCAATG
BsrFI 1534 GCCGGT [GA]^CCGG[CT]
2164 ACCGGT
BstUI 679 CGCG CG^CG
2089 CGCG
CfoI 19 GCGC GCG^C
2088 GCGC
Cfr10I 1534 GCCGGT [GA]^CCGG[CT]
2164 ACCGGT
DraI 1995 TTTAAA TTT^AAA
2310 TTTAAA
EaeI 453 TGGCCA [CT]^GGCC[GA]
1100 CGGCCG
EcoRI 1418 GAATTC G^AATTC
1742 GAATTC
HhaI 19 GCGC GCG^C
2088 GCGC
HinP1I 19 GCGC G^CGC
2088 GCGC
HincII 1437 GTCAAC GT[CT]^[GA]AC
1545 GTTGAC
HindII 1437 GTCAAC GT[CT]^[GA]AC
1545 GTTGAC
MvnI 679 CGCG CG^CG
2089 CGCG
NciI 692 CCCGG CC^[GC]GG
2244 CCGGG
PflFI 478 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
754 GACATTGTC
PflMI 1894 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2479 CCACACCCTGG
Tth111I 478 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
754 GACATTGTC
Van91I 1894 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2479 CCACACCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaII BanI
BbvI BcgI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MspI
MunI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII