Restriction map for
pF1KB4702
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspMI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
KasI KpnI KspI MamI MluI
MroI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 37 GTAGAC GT^[AC][GT]AC
AciI 31 GCGG CCGC(-3/-1)
AfaI 829 GTAC GT^AC
AflIII 825 ACATGT A^C[GA][CT]GT
AlwI 675 GATCC GGATC(4/5)
AlwNI 833 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AvrII 240 CCTAGG C^CTAGG
BalI 153 TGGCCA TGG^CCA
BglI 47 GCCGGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 240 CCTAGG C^CTAGG
BseRI 779 GAGGAG GAGGAG(10/8)
BsgI 763 GTGCAG GTGCAG(16/14)
BspLU11I 825 ACATGT A^CATGT
BsrBI 29 GAGCGG CCGCTC(-3/-3)
BsrDI 1097 CATTGC GCAATG(2/0)
BsrFI 47 GCCGGC [GA]^CCGG[CT]
BstYI 674 AGATCC [GA]^GATC[CT]
Cfr10I 47 GCCGGC [GA]^CCGG[CT]
EcoRI 1282 GAATTC G^AATTC
EcoRV 354 GATATC GAT^ATC
EcoT14I 240 CCTAGG C^C[AT][AT]GG
FauI 31 GCGGG CCCGC(4/6)
HincII 1491 GTTAAC GT[CT]^[GA]AC
HindII 1491 GTTAAC GT[CT]^[GA]AC
HpaI 1491 GTTAAC GTT^AAC
MfeI 1304 CAATTG C^AATTG
MflI 674 AGATCC [GA]^GATC[CT]
MluNI 153 TGGCCA TGG^CCA
MscI 153 TGGCCA TGG^CCA
MspA1I 68 CAGCTG C[AC]G^C[GT]G
MunI 1304 CAATTG C^AATTG
NaeI 47 GCCGGC GCC^GGC
NgoMIV 47 GCCGGC G^CCGGC
NlaIV 657 GGAACC GG[ACGT]^[ACGT]CC
PflMI 1300 CCAACAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 68 CAGCTG CAG^CTG
RsaI 829 GTAC GT^AC
SapI 200 GAAGAGC GCTCTTC(1/4)
SexAI 542 ACCAGGT A^CC[AT]GGT
SphI 549 GCATGC GCATG^C
StyI 240 CCTAGG C^C[AT][AT]GG
Van91I 1300 CCAACAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 674 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 713 GGACC G^G[AT]CC
1198 GGACC
BcnI 60 CCGGG CC^[GC]GG
851 CCCGG
BsiYI 190 CCTCTTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1300 CCAACAATTGG
BslI 190 CCTCTTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1300 CCAACAATTGG
BsmI 846 GAATGC GAATGC(1/-1)
645 GCATTC
BspHI 168 TCATGA T^CATGA
516 TCATGA
BsrI 1089 ACTGG ACTGG(1/-1)
1676 ACTGG
BstAPI 540 GCACCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
841 GCAGAGAATGC
DraIII 417 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
541 CACCAGGTG
EarI 191 CTCTTC CTCTTC(1/4)
200 GAAGAG
HinfI 247 GATTC G^A[ACGT]TC
343 GAATC
HphI 244 GGTGA GGTGA(8/7)
396 TCACC
Ksp632I 191 CTCTTC CTCTTC(1/4)
200 GAAGAG
MaeII 1225 ACGT A^CGT
1316 ACGT
MslI 596 CACTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
937 CATGTTCGTG
NciI 60 CCGGG CC^[GC]GG
851 CCCGG
NspI 549 GCATGC [GA]CATG^[CT]
825 ACATGT
RcaI 168 TCATGA T^CATGA
516 TCATGA
SmlI 452 CTCAAG C^T[CT][GA]AG
1415 CTCAAG
TfiI 247 GATTC G^A[AT]TC
343 GAATC
Tsp45I 638 GTCAC ^GT[GC]AC
1165 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AluI ApoI AspHI BbvI
BfaI BmyI BpmI BsaJI BsiHKAI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HpaII ItaI MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I