Restriction map for
pF1KB4745
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AflII AflIII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 838 GTCTAC GT^[AC][GT]AC
AccIII 590 TCCGGA T^CCGGA
AcyI 870 GACGCC G[GA]^CG[CT]C
AfaI 666 GTAC GT^AC
Alw44I 971 GTGCAC G^TGCAC
Aor51HI 419 AGCGCT AGC^GCT
ApaI 337 GGGCCC GGGCC^C
ApaLI 971 GTGCAC G^TGCAC
AvaI 345 CCCGGG C^[CT]CG[GA]G
BbrPI 37 CACGTG CAC^GTG
BsaAI 37 CACGTG [CT]AC^GT[GA]
BsaHI 870 GACGCC G[GA]^CG[CT]C
BsaI 12 GGTCTC GGTCTC(1/5)
BsaWI 590 TCCGGA [AT]^CCGG[AT]
BseAI 590 TCCGGA T^CCGGA
BsmBI 1080 CGTCTC CGTCTC(1/5)
BsoBI 345 CCCGGG C^[CT]CG[GA]G
BspEI 590 TCCGGA T^CCGGA
BspMI 411 GCAGGT ACCTGC(4/8)
BsrBI 619 GAGCGG CCGCTC(-3/-3)
BsrI 334 ACTGG ACTGG(1/-1)
BstAPI 603 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 581 AGATCC [GA]^GATC[CT]
CfoI 420 GCGC GCG^C
DrdI 1072 GACCTGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 419 AGCGCT AGC^GCT
EcoNI 588 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 992 CCATGG C^C[AT][AT]GG
HaeII 419 AGCGCT [GA]GCGC^[CT]
HgaI 870 GACGC GACGC(5/10)
HhaI 420 GCGC GCG^C
Hin1I 870 GACGCC G[GA]^CG[CT]C
HinP1I 420 GCGC G^CGC
HincII 928 GTCAAC GT[CT]^[GA]AC
HindII 928 GTCAAC GT[CT]^[GA]AC
MboII 261 GAAGA GAAGA(8/7)
MflI 581 AGATCC [GA]^GATC[CT]
MroI 590 TCCGGA T^CCGGA
NcoI 992 CCATGG C^CATGG
PflMI 141 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 130 GACTC GAGTC(4/5)
PmaCI 37 CACGTG CAC^GTG
PmlI 37 CACGTG CAC^GTG
RsaI 666 GTAC GT^AC
SexAI 710 ACCTGGT A^CC[AT]GGT
SfaNI 731 GCATC GCATC(5/9)
SmaI 345 CCCGGG CCC^GGG
StyI 992 CCATGG C^C[AT][AT]GG
TaqI 660 TCGA T^CGA
TthHB8I 660 TCGA T^CGA
Van91I 141 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 905 CCAACATCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 581 AGATCC [GA]^GATC[CT]
XmaCI 345 CCCGGG C^CCGGG
XmaI 345 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 582 GATCC GGATC(4/5)
68 GGATC
AlwNI 91 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
604 CAGCTGCTG
BfaI 241 CTAG C^TAG
778 CTAG
BstXI 704 CCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1017 CCAAATCAGTGG
DraIII 37 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
142 CACAATGTG
FauI 65 GCGGG CCCGC(4/6)
621 GCGGG
MaeI 241 CTAG C^TAG
778 CTAG
MseI 356 TTAA T^TAA
482 TTAA
NaeI 892 GCCGGC GCC^GGC
1040 GCCGGC
NgoMIV 892 GCCGGC G^CCGGC
1040 GCCGGC
PpuMI 4 GGGTCCC [GA]G^G[AT]CC[CT]
1070 GGGACCT
PstI 88 CTGCAG CTGCA^G
280 CTGCAG
PvuII 85 CAGCTG CAG^CTG
604 CAGCTG
SacI 29 GAGCTC GAGCT^C
547 GAGCTC
SfcI 88 CTGCAG C^T[GA][CT]AG
280 CTGCAG
StuI 389 AGGCCT AGG^CCT
956 AGGCCT
TfiI 22 GAATC G^A[AT]TC
717 GATTC
Tru9I 356 TTAA T^TAA
482 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BssKI BstNI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI Tsp45I TspRI