################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII BalI BamHI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII ItaI KasI KpnI KspI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI Sau96I SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AvrII 205 CCTAGG C^CTAGG BanI 1271 GGCACC G^G[CT][GA]CC BfaI 206 CTAG C^TAG BlnI 205 CCTAGG C^CTAGG BsaBI 662 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 205 CCTAGG C^C[ACGT][ACGT]GG BsgI 444 CTGCAC GTGCAG(16/14) BsmBI 349 CGTCTC CGTCTC(1/5) BsmI 823 GCATTC GAATGC(1/-1) BspLU11I 550 ACATGT A^CATGT BspMI 1342 ACCTGC ACCTGC(4/8) BstBI 911 TTCGAA TT^CGAA DpnII 668 GATC ^GATC EcoNI 228 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 205 CCTAGG C^C[AT][AT]GG EcoT22I 176 ATGCAT ATGCA^T HindIII 318 AAGCTT A^AGCTT MaeI 206 CTAG C^TAG MaeII 348 ACGT A^CGT MamI 662 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 668 GATC ^GATC NdeII 668 GATC ^GATC NsiI 176 ATGCAT ATGCA^T NspV 911 TTCGAA TT^CGAA SacI 498 GAGCTC GAGCT^C SapI 902 GCTCTTC GCTCTTC(1/4) Sau3AI 668 GATC ^GATC SfcI 362 CTACAG C^T[GA][CT]AG SfuI 911 TTCGAA TT^CGAA SmlI 333 CTCAAG C^T[CT][GA]AG StyI 205 CCTAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 744 CCGC CCGC(-3/-1) 1500 CCGC AflIII 346 ACACGT A^C[GA][CT]GT 550 ACATGT AspHI 498 GAGCTC G[AT]GC[AT]^C 1170 GAGCAC BanII 498 GAGCTC G[GA]GC[CT]^C 1086 GAGCCC BsaI 540 GAGACC GGTCTC(1/5) 1212 GAGACC BsiHKAI 498 GAGCTC G[AT]GC[AT]^C 1170 GAGCAC BsiYI 228 CCTTTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 996 CCAAACAAAGG BslI 228 CCTTTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 996 CCAAACAAAGG BsrDI 722 GCAATG GCAATG(2/0) 1310 GCAATG BssKI 52 CCAGG ^CC[ACGT]GG 234 CCAGG BstNI 52 CCAGG CC^[AT]GG 234 CCAGG Cac8I 75 GCCTGC GC[ACGT]^[ACGT]GC 320 GCTTGC EcoRII 52 CCAGG ^CC[AT]GG 234 CCAGG MseI 1100 TTAA T^TAA 1331 TTAA MvaI 52 CCAGG CC^[AT]GG 234 CCAGG NlaIV 131 GGTTCC GG[ACGT]^[ACGT]CC 1271 GGCACC NspI 178 GCATGT [GA]CATG^[CT] 550 ACATGT PleI 106 GACTC GAGTC(4/5) 372 GAGTC ScrFI 52 CCAGG CC^[ACGT]GG 234 CCAGG TfiI 154 GATTC G^A[AT]TC 220 GAATC Tru9I 1100 TTAA T^TAA 1331 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AcsI AluI ApoI Asp700I BmyI BpmI BsmAI Bsp1286I BsrI BstF5I DdeI EarI EcoRI FokI HinfI HphI Ksp632I MaeIII MboII MnlI MslI NdeI NlaIII SfaNI TaqI Tsp45I Tsp509I TspRI TthHB8I XmnI