Restriction map for
pF1KB4806
##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AclI AflII AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
BalI BbeI BcgI BciVI BclI
BfrI BsgI BsiEI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssSI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I FbaI FseI HincII HindII
HpaI KasI KspI MfeI MluI
MluNI MscI MunI NarI NheI
NotI NspV PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SnaBI SpeI SphI SspBI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 1226 GTCTAC GT^[AC][GT]AC
AccIII 674 TCCGGA T^CCGGA
AcsI 21 GAATTC [GA]^AATT[CT]
AflIII 2954 ACACGT A^C[GA][CT]GT
AgeI 463 ACCGGT A^CCGGT
Aor51HI 997 AGCGCT AGC^GCT
ApoI 21 GAATTC [GA]^AATT[CT]
AspI 2815 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1658 TGCGCA TGC^GCA
AvrII 2965 CCTAGG C^CTAGG
BbrPI 2611 CACGTG CAC^GTG
BglII 1479 AGATCT A^GATCT
BlnI 2965 CCTAGG C^CTAGG
BsaAI 2611 CACGTG [CT]AC^GT[GA]
BsaBI 2811 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 674 TCCGGA T^CCGGA
BsmBI 332 GAGACG CGTCTC(1/5)
BsmI 1835 GCATTC GAATGC(1/-1)
BspEI 674 TCCGGA T^CCGGA
BspHI 2486 TCATGA T^CATGA
BsrDI 2564 GCAATG GCAATG(2/0)
BssHII 2028 GCGCGC G^CGCGC
BstEII 1318 GGTCACC G^GT[ACGT]ACC
BstPI 1318 GGTCACC G^GT[ACGT]ACC
BstXI 36 CCACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 587 CGGCCA [CT]^GGCC[GA]
Eco47III 997 AGCGCT AGC^GCT
EcoO65I 1318 GGTCACC G^GT[ACGT]ACC
EcoRI 21 GAATTC G^AATTC
EcoRV 501 GATATC GAT^ATC
EcoT22I 1361 ATGCAT ATGCA^T
FspI 1658 TGCGCA TGC^GCA
HindIII 1843 AAGCTT A^AGCTT
MamI 2811 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 674 TCCGGA T^CCGGA
NcoI 2591 CCATGG C^CATGG
NdeI 1499 CATATG CA^TATG
NruI 2128 TCGCGA TCG^CGA
NsiI 1361 ATGCAT ATGCA^T
PflFI 2815 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 463 ACCGGT A^CCGGT
PmaCI 2611 CACGTG CAC^GTG
PmlI 2611 CACGTG CAC^GTG
RcaI 2486 TCATGA T^CATGA
SacI 831 GAGCTC GAGCT^C
SbfI 161 CCTGCAGG CCTGCA^GG
SgrAI 2449 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 161 CCTGCAGG CCTGCA^GG
StuI 3173 AGGCCT AGG^CCT
Tth111I 2815 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 167 GGTACC G^GTACC
466 GGTACC
AocI 804 CCTCAGG CC^T[ACGT]AGG
1594 CCTCAGG
Asp700I 311 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2541 GAAGACATTC
Asp718I 167 GGTACC G^GTACC
466 GGTACC
BamHI 851 GGATCC G^GATCC
1432 GGATCC
BbvCI 155 CCTCAGC CCTCAGC(-5/-2)
1791 CCTCAGC
BglI 437 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2399 GCCGCCTTGGC
BsrBI 136 CCGCTC CCGCTC(-3/-3)
2077 GAGCGG
Bsu36I 804 CCTCAGG CC^T[ACGT]AGG
1594 CCTCAGG
DraIII 2740 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
3137 CACAGAGTG
DsaI 36 CCACGG C^C[GA][CT]GG
2591 CCATGG
Eco81I 804 CCTCAGG CC^T[ACGT]AGG
1594 CCTCAGG
EcoNI 800 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2433 CCTAGATGAGG
KpnI 167 GGTACC GGTAC^C
466 GGTACC
MaeII 2612 ACGT A^CGT
2956 ACGT
NaeI 975 GCCGGC GCC^GGC
2450 GCCGGC
NgoMIV 975 GCCGGC G^CCGGC
2450 GCCGGC
SapI 1526 GAAGAGC GCTCTTC(1/4)
1683 GAAGAGC
ScaI 2093 AGTACT AGT^ACT
3317 AGTACT
Tsp45I 1288 GTCAC ^GT[GC]AC
1319 GTCAC
XmnI 311 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2541 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI