Restriction map for
pF1KB4848
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AhdI AlwI
AocI ApaI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BfrI BglII BlnI
BsaAI BsaBI BsaHI BseAI BsgI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 215 GGTACC G^GTACC
AgeI 212 ACCGGT A^CCGGT
Alw44I 184 GTGCAC G^TGCAC
Aor51HI 152 AGCGCT AGC^GCT
ApaLI 184 GTGCAC G^TGCAC
Asp718I 215 GGTACC G^GTACC
BanI 215 GGTACC G^G[CT][GA]CC
BbsI 274 GTCTTC GAAGAC(2/6)
BbvCI 1168 GCTGAGG CCTCAGC(-5/-2)
BciVI 969 GGATAC GTATCC(6/5)
BclI 821 TGATCA T^GATCA
BglI 1215 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1129 CTCCAG CTGGAG(16/14)
BpuAI 274 GTCTTC GAAGAC(2/6)
BsaWI 212 ACCGGT [AT]^CCGG[AT]
BsiEI 795 CGACCG CG[GA][CT]^CG
BsiWI 115 CGTACG C^GTACG
BsmI 18 GAATGC GAATGC(1/-1)
BspMI 943 ACCTGC ACCTGC(4/8)
BssSI 832 CACGAG CACGAG(-5/-1)
BstAPI 938 GCATGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 346 GGTGACC G^GT[ACGT]ACC
BstPI 346 GGTGACC G^GT[ACGT]ACC
DraII 59 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 682 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 128 TGGCCG [CT]^GGCC[GA]
Eco47III 152 AGCGCT AGC^GCT
EcoO109I 59 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 346 GGTGACC G^GT[ACGT]ACC
FbaI 821 TGATCA T^GATCA
KpnI 215 GGTACC GGTAC^C
NaeI 1121 GCCGGC GCC^GGC
NcoI 281 CCATGG C^CATGG
NgoMIV 1121 GCCGGC G^CCGGC
NspI 554 GCATGT [GA]CATG^[CT]
PinAI 212 ACCGGT A^CCGGT
PpuMI 59 GGGACCC [GA]G^G[AT]CC[CT]
SacI 957 GAGCTC GAGCT^C
SmlI 250 CTCAAG C^T[CT][GA]AG
StuI 692 AGGCCT AGG^CCT
XbaI 912 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 88 CGCG CG^CG
119 CGCG
AlwNI 1080 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1187 CAGAGGCTG
BbvI 879 GCTGC GCAGC(8/12)
1082 GCTGC
BfaI 14 CTAG C^TAG
913 CTAG
BlpI 874 GCTCAGC GC^T[ACGT]AGC
1192 GCTGAGC
Bpu1102I 874 GCTCAGC GC^T[ACGT]AGC
1192 GCTGAGC
BsrBI 168 GAGCGG CCGCTC(-3/-3)
587 GAGCGG
BsrDI 102 CATTGC GCAATG(2/0)
330 CATTGC
BsrFI 212 ACCGGT [GA]^CCGG[CT]
1121 GCCGGC
BsrI 1003 CCAGT ACTGG(1/-1)
1131 CCAGT
BstUI 88 CGCG CG^CG
119 CGCG
CelII 874 GCTCAGC GC^T[ACGT]AGC
1192 GCTGAGC
Cfr10I 212 ACCGGT [GA]^CCGG[CT]
1121 GCCGGC
DsaI 189 CCACGG C^C[GA][CT]GG
281 CCATGG
FauI 86 CCCGC CCCGC(4/6)
630 GCGGG
HaeII 152 AGCGCT [GA]GCGC^[CT]
257 GGCGCT
MaeI 14 CTAG C^TAG
913 CTAG
MspA1I 877 CAGCTG C[AC]G^C[GT]G
1080 CAGCTG
MvnI 88 CGCG CG^CG
119 CGCG
PflMI 30 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
681 CCACATCGTGG
PleI 47 GAGTC GAGTC(4/5)
464 GAGTC
PvuII 877 CAGCTG CAG^CTG
1080 CAGCTG
SapI 165 GAAGAGC GCTCTTC(1/4)
846 GAAGAGC
SfaNI 1183 GATGC GCATC(5/9)
882 GCATC
TseI 879 GCTGC G^C[AT]GC
1082 GCTGC
Van91I 30 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
681 CCACATCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BcnI BmyI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI StyI TfiI Tsp45I
TspRI