Restriction map for
pF1KB4877
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstPI
Bsu36I CelII ClaI CpoI DraIII
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1359 GTATAC GT^[AC][GT]AC
AfaI 720 GTAC GT^AC
AflIII 1162 ACATGT A^C[GA][CT]GT
ApaI 354 GGGCCC GGGCC^C
BalI 2055 TGGCCA TGG^CCA
BanI 594 GGCGCC G^G[CT][GA]CC
BbeI 594 GGCGCC GGCGC^C
BbvCI 757 CCTCAGC CCTCAGC(-5/-2)
BglII 1221 AGATCT A^GATCT
BsaI 85 GGTCTC GGTCTC(1/5)
BsgI 1301 GTGCAG GTGCAG(16/14)
BsmBI 856 CGTCTC CGTCTC(1/5)
BsmI 2267 GAATGC GAATGC(1/-1)
BspLU11I 1162 ACATGT A^CATGT
BspMI 1561 GCAGGT ACCTGC(4/8)
BsrBI 290 CCGCTC CCGCTC(-3/-3)
Bst1107I 1359 GTATAC GTA^TAC
BstXI 1623 CCAGTGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1221 AGATCT [GA]^GATC[CT]
BstZ17I 1359 GTATAC GTA^TAC
DraI 838 TTTAAA TTT^AAA
DraII 1410 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 1686 GACAAATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1410 GGGGCCT [GA]G^G[ACGT]CC[CT]
KasI 594 GGCGCC G^GCGCC
MflI 1221 AGATCT [GA]^GATC[CT]
MluNI 2055 TGGCCA TGG^CCA
MscI 2055 TGGCCA TGG^CCA
NarI 594 GGCGCC GG^CGCC
PaeR7I 220 CTCGAG C^TCGAG
PflMI 1459 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 79 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 720 GTAC GT^AC
SfaNI 916 GCATC GCATC(5/9)
SfoI 594 GGCGCC GGC^GCC
SgrAI 494 CGCCGGCG C[GA]^CCGG[CT]G
StuI 216 AGGCCT AGG^CCT
Van91I 1459 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2058 CCATAGAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 220 CTCGAG C^TCGAG
XhoII 1221 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 68 GACGCC G[GA]^CG[CT]C
594 GGCGCC
AlwI 1389 GGATC GGATC(4/5)
1680 GGATC
AlwNI 1460 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1583 CAGATACTG
AvaII 1402 GGACC G^G[AT]CC
1839 GGTCC
BbsI 385 GAAGAC GAAGAC(2/6)
1774 GAAGAC
BfaI 178 CTAG C^TAG
316 CTAG
BglI 334 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
538 GCCAAGGAGGC
BpmI 112 CTCCAG CTGGAG(16/14)
905 CTGGAG
BpuAI 385 GAAGAC GAAGAC(2/6)
1774 GAAGAC
BsaHI 68 GACGCC G[GA]^CG[CT]C
594 GGCGCC
BsiEI 236 CGGCCG CG[GA][CT]^CG
344 CGGCCG
BsrFI 238 GCCGGC [GA]^CCGG[CT]
495 GCCGGC
BssSI 689 CACGAG CACGAG(-5/-1)
2204 CTCGTG
BstF5I 917 CATCC GGATG(2/0)
1368 CATCC
Cfr10I 238 GCCGGC [GA]^CCGG[CT]
495 GCCGGC
EagI 236 CGGCCG C^GGCCG
344 CGGCCG
EclXI 236 CGGCCG C^GGCCG
344 CGGCCG
Eco52I 236 CGGCCG C^GGCCG
344 CGGCCG
EcoNI 219 CCTCGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1866 CCTCCCAGAGG
FokI 917 CATCC GGATG(9/13)
1368 CATCC
HaeII 203 GGCGCT [GA]GCGC^[CT]
594 GGCGCC
HgaI 68 GACGC GACGC(5/10)
501 GACGC
Hin1I 68 GACGCC G[GA]^CG[CT]C
594 GGCGCC
MaeI 178 CTAG C^TAG
316 CTAG
MaeII 1376 ACGT A^CGT
2067 ACGT
NaeI 238 GCCGGC GCC^GGC
495 GCCGGC
NcoI 155 CCATGG C^CATGG
2386 CCATGG
NgoMIV 238 GCCGGC G^CCGGC
495 GCCGGC
SmaI 163 CCCGGG CCC^GGG
601 CCCGGG
Tsp45I 586 GTGAC ^GT[GC]AC
1032 GTCAC
XmaCI 163 CCCGGG C^CCGGG
601 CCCGGG
XmaI 163 CCCGGG C^CCGGG
601 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
AspHI AvaI BanII BbvI BcnI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI SacI SacII
SapI Sau3AI Sau96I ScrFI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I