Restriction map for
pF1KB4895
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FauI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspI PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AciI 615 GCGG CCGC(-3/-1)
AgeI 982 ACCGGT A^CCGGT
AhdI 1327 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1240 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 1327 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 700 GGTGCC G^G[CT][GA]CC
BanII 1072 GAGCTC G[GA]GC[CT]^C
BclI 816 TGATCA T^GATCA
BcnI 637 CCGGG CC^[GC]GG
BglII 545 AGATCT A^GATCT
BpmI 1320 CTCCAG CTGGAG(16/14)
BsaI 785 GAGACC GGTCTC(1/5)
BsaWI 982 ACCGGT [AT]^CCGG[AT]
BseRI 671 GAGGAG GAGGAG(10/8)
BsgI 469 GTGCAG GTGCAG(16/14)
BsmAI 785 GAGAC GTCTC(1/5)
Bsp1407I 870 TGTACA T^GTACA
BspHI 1011 TCATGA T^CATGA
BsrFI 982 ACCGGT [GA]^CCGG[CT]
BsrGI 870 TGTACA T^GTACA
BstBI 572 TTCGAA TT^CGAA
BstEII 884 GGTTACC G^GT[ACGT]ACC
BstPI 884 GGTTACC G^GT[ACGT]ACC
Cfr10I 982 ACCGGT [GA]^CCGG[CT]
DsaI 230 CCATGG C^C[GA][CT]GG
EaeI 616 CGGCCA [CT]^GGCC[GA]
Eam1105I 1327 GACTATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 626 CCTATGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 884 GGTTACC G^GT[ACGT]ACC
EcoRI 1143 GAATTC G^AATTC
EcoT14I 230 CCATGG C^C[AT][AT]GG
FbaI 816 TGATCA T^GATCA
HaeII 1168 GGCGCT [GA]GCGC^[CT]
MaeII 245 ACGT A^CGT
NciI 637 CCGGG CC^[GC]GG
NcoI 230 CCATGG C^CATGG
NheI 1234 GCTAGC G^CTAGC
NspV 572 TTCGAA TT^CGAA
PflMI 1182 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 982 ACCGGT A^CCGGT
PleI 350 GACTC GAGTC(4/5)
PvuII 1243 CAGCTG CAG^CTG
RcaI 1011 TCATGA T^CATGA
SacI 1072 GAGCTC GAGCT^C
SapI 1074 GCTCTTC GCTCTTC(1/4)
SfuI 572 TTCGAA TT^CGAA
SspBI 870 TGTACA T^GTACA
StuI 500 AGGCCT AGG^CCT
StyI 230 CCATGG C^C[AT][AT]GG
TfiI 184 GAATC G^A[AT]TC
Tsp45I 1325 GTGAC ^GT[GC]AC
Van91I 1182 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 105 GATCC GGATC(4/5)
586 GATCC
BfaI 1217 CTAG C^TAG
1235 CTAG
BlpI 6 GCTGAGC GC^T[ACGT]AGC
65 GCTCAGC
Bpu1102I 6 GCTGAGC GC^T[ACGT]AGC
65 GCTCAGC
BsmFI 234 GGGAC GGGAC(10/14)
623 GTCCC
BstNI 56 CCTGG CC^[AT]GG
619 CCAGG
BstYI 104 AGATCC [GA]^GATC[CT]
545 AGATCT
CelII 6 GCTGAGC GC^T[ACGT]AGC
65 GCTCAGC
CfoI 84 GCGC GCG^C
1169 GCGC
EcoRII 56 CCTGG ^CC[AT]GG
619 CCAGG
HhaI 84 GCGC GCG^C
1169 GCGC
HinP1I 84 GCGC G^CGC
1169 GCGC
HinfI 184 GAATC G^A[ACGT]TC
350 GACTC
MaeI 1217 CTAG C^TAG
1235 CTAG
MflI 104 AGATCC [GA]^GATC[CT]
545 AGATCT
MspA1I 613 CAGCGG C[AC]G^C[GT]G
1243 CAGCTG
MvaI 56 CCTGG CC^[AT]GG
619 CCAGG
PpuMI 621 AGGTCCC [GA]G^G[AT]CC[CT]
1227 AGGTCCT
PstI 1091 CTGCAG CTGCA^G
1309 CTGCAG
TaqI 573 TCGA T^CGA
594 TCGA
TthHB8I 573 TCGA T^CGA
594 TCGA
XcmI 1212 CCAGTCTAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1239 CCAGCAGCTGAGTGG
XhoII 104 AGATCC [GA]^GATC[CT]
545 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
AvaII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MslI MspI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp509I TspRI