Restriction map for
pF1KB4907
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NarI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 270 GACGTC GACGT^C
AccI 586 GTCTAC GT^[AC][GT]AC
AccII 1137 CGCG CG^CG
AcsI 519 GAATTT [GA]^AATT[CT]
AlwI 769 GGATC GGATC(4/5)
AlwNI 311 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 436 GGGCCC GGGCC^C
ApoI 519 GAATTT [GA]^AATT[CT]
AspHI 894 GAGCAC G[AT]GC[AT]^C
AvaI 683 CCCGAG C^[CT]CG[GA]G
BanI 576 GGTGCC G^G[CT][GA]CC
BbsI 354 GTCTTC GAAGAC(2/6)
BciVI 944 GTATCC GTATCC(6/5)
BpuAI 354 GTCTTC GAAGAC(2/6)
BsaI 122 GGTCTC GGTCTC(1/5)
BsaWI 1010 ACCGGA [AT]^CCGG[AT]
BseRI 321 CTCCTC GAGGAG(10/8)
BsiHKAI 894 GAGCAC G[AT]GC[AT]^C
BsmBI 909 GAGACG CGTCTC(1/5)
BsoBI 683 CCCGAG C^[CT]CG[GA]G
Bsp1407I 980 TGTACA T^GTACA
BspMI 731 ACCTGC ACCTGC(4/8)
BsrFI 78 GCCGGC [GA]^CCGG[CT]
BsrGI 980 TGTACA T^GTACA
BstAPI 474 GCAGTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 602 GGTCACC G^GT[ACGT]ACC
BstPI 602 GGTCACC G^GT[ACGT]ACC
BstUI 1137 CGCG CG^CG
BstYI 769 GGATCT [GA]^GATC[CT]
CfoI 1136 GCGC GCG^C
Cfr10I 78 GCCGGC [GA]^CCGG[CT]
EcoO65I 602 GGTCACC G^GT[ACGT]ACC
EcoT14I 660 CCATGG C^C[AT][AT]GG
HhaI 1136 GCGC GCG^C
HinP1I 1136 GCGC G^CGC
HincII 417 GTCAAC GT[CT]^[GA]AC
HindII 417 GTCAAC GT[CT]^[GA]AC
MflI 769 GGATCT [GA]^GATC[CT]
MspA1I 593 CAGCTG C[AC]G^C[GT]G
MvnI 1137 CGCG CG^CG
NaeI 78 GCCGGC GCC^GGC
NcoI 660 CCATGG C^CATGG
NgoMIV 78 GCCGGC G^CCGGC
PvuII 593 CAGCTG CAG^CTG
SfcI 212 CTGTAG C^T[GA][CT]AG
SspBI 980 TGTACA T^GTACA
StuI 687 AGGCCT AGG^CCT
StyI 660 CCATGG C^C[AT][AT]GG
TaqI 843 TCGA T^CGA
TthHB8I 843 TCGA T^CGA
XhoII 769 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 270 GACGTC G[GA]^CG[CT]C
902 GACGCC
Asp700I 154 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1122 GAAGCCATTC
AvaII 678 GGACC G^G[AT]CC
831 GGTCC
BanII 190 GAGCCC G[GA]GC[CT]^C
436 GGGCCC
BbvI 1151 GCAGC GCAGC(8/12)
1058 GCTGC
BfaI 359 CTAG C^TAG
367 CTAG
BpmI 431 CTCCAG CTGGAG(16/14)
930 CTCCAG
BsaHI 270 GACGTC G[GA]^CG[CT]C
902 GACGCC
BsgI 1059 CTGCAC GTGCAG(16/14)
1180 CTGCAC
BsiYI 558 CCCCGTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
932 CCAGAAAACGG
BslI 558 CCCCGTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
932 CCAGAAAACGG
BsmAI 909 GAGAC GTCTC(1/5)
123 GTCTC
Cac8I 78 GCCGGC GC[ACGT]^[ACGT]GC
470 GCCAGC
DraII 435 AGGGCCC [GA]G^G[ACGT]CC[CT]
436 GGGCCCC
DsaI 560 CCGTGG C^C[GA][CT]GG
660 CCATGG
EarI 345 CTCTTC CTCTTC(1/4)
15 GAAGAG
EcoO109I 435 AGGGCCC [GA]G^G[ACGT]CC[CT]
436 GGGCCCC
HgaI 902 GACGC GACGC(5/10)
406 GCGTC
Hin1I 270 GACGTC G[GA]^CG[CT]C
902 GACGCC
Ksp632I 345 CTCTTC CTCTTC(1/4)
15 GAAGAG
MaeI 359 CTAG C^TAG
367 CTAG
MaeII 131 ACGT A^CGT
271 ACGT
MwoI 474 GCAGTTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
896 GCACAAGACGC
NdeI 10 CATATG CA^TATG
1195 CATATG
TfiI 109 GAATC G^A[AT]TC
919 GAATC
TseI 1058 GCTGC G^C[AT]GC
1151 GCAGC
Tsp509I 520 AATT ^AATT
873 AATT
TspRI 242 CAGTG CA[GC]TG[ACGT][ACGT]^
717 CAGTG
XmnI 154 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1122 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI BcnI BmyI
BsaJI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI Tru9I Tsp45I