Restriction map for
pF1KB4940
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BsaBI BsiWI BsmBI BsmI
BspDI BspHI BspLU11I BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MflI MluI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI StyI SwaI TfiI Tru9I
Tth111I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 348 TCCGGA T^CCGGA
AcsI 1306 AAATTC [GA]^AATT[CT]
Alw44I 852 GTGCAC G^TGCAC
AlwI 459 GATCC GGATC(4/5)
AlwNI 981 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1033 GGGCCC GGGCC^C
ApaLI 852 GTGCAC G^TGCAC
ApoI 1306 AAATTC [GA]^AATT[CT]
BbrPI 816 CACGTG CAC^GTG
BpmI 220 CTGGAG CTGGAG(16/14)
BsaAI 816 CACGTG [CT]AC^GT[GA]
BsaWI 348 TCCGGA [AT]^CCGG[AT]
BseAI 348 TCCGGA T^CCGGA
BsgI 902 CTGCAC GTGCAG(16/14)
Bsp1407I 632 TGTACA T^GTACA
BspEI 348 TCCGGA T^CCGGA
BsrDI 884 GCAATG GCAATG(2/0)
BsrGI 632 TGTACA T^GTACA
BssHII 428 GCGCGC G^CGCGC
BstXI 212 CCACAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1032 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 775 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 25 GACATGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 206 CCGTGG C^C[GA][CT]GG
EcoO109I 1032 GGGGCCC [GA]G^G[ACGT]CC[CT]
MroI 348 TCCGGA T^CCGGA
NaeI 897 GCCGGC GCC^GGC
NgoMIV 897 GCCGGC G^CCGGC
NspI 1121 GCATGC [GA]CATG^[CT]
PflMI 774 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 816 CACGTG CAC^GTG
PmlI 816 CACGTG CAC^GTG
PstI 441 CTGCAG CTGCA^G
PvuI 1156 CGATCG CGAT^CG
SfaNI 1065 GATGC GCATC(5/9)
SfcI 441 CTGCAG C^T[GA][CT]AG
SphI 1121 GCATGC GCATG^C
SspBI 632 TGTACA T^GTACA
StuI 507 AGGCCT AGG^CCT
TaqI 409 TCGA T^CGA
TthHB8I 409 TCGA T^CGA
Van91I 774 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 858 CCACTTGGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 633 GTAC GT^AC
723 GTAC
AhdI 96 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
951 GACCCTGGGTC
AspEI 96 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
951 GACCCTGGGTC
BalI 209 TGGCCA TGG^CCA
888 TGGCCA
BbsI 93 GAAGAC GAAGAC(2/6)
1151 GAAGAC
BlpI 702 GCTGAGC GC^T[ACGT]AGC
972 GCTGAGC
Bpu1102I 702 GCTGAGC GC^T[ACGT]AGC
972 GCTGAGC
BpuAI 93 GAAGAC GAAGAC(2/6)
1151 GAAGAC
BsaI 283 GAGACC GGTCTC(1/5)
843 GGTCTC
BsmAI 283 GAGAC GTCTC(1/5)
844 GTCTC
CelII 702 GCTGAGC GC^T[ACGT]AGC
972 GCTGAGC
EagI 177 CGGCCG C^GGCCG
895 CGGCCG
Eam1105I 96 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
951 GACCCTGGGTC
EclXI 177 CGGCCG C^GGCCG
895 CGGCCG
Eco52I 177 CGGCCG C^GGCCG
895 CGGCCG
MaeIII 989 GTCAC ^GT[ACGT]AC
1283 GTGAC
MluNI 209 TGGCCA TGG^CCA
888 TGGCCA
MscI 209 TGGCCA TGG^CCA
888 TGGCCA
MslI 1186 CACACTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1258 CACACAGGTG
MspA1I 126 CCGCTG C[AC]G^C[GT]G
414 CAGCGG
RsaI 633 GTAC GT^AC
723 GTAC
SmaI 477 CCCGGG CCC^GGG
1029 CCCGGG
SmlI 961 CTTGAG C^T[CT][GA]AG
1237 CTCAAG
Tsp45I 989 GTCAC ^GT[GC]AC
1283 GTGAC
XmaCI 477 CCCGGG C^CCGGG
1029 CCCGGG
XmaI 477 CCCGGG C^CCGGG
1029 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII EaeI EarI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MboI MboII
MnlI MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI SacI Sau3AI Sau96I ScrFI
SfoI TseI Tsp509I TspRI