Restriction map for
pF1KB4944
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BseRI BsgI BsiWI BsmBI
BspDI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRII
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MscI MspA1I MunI MvaI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 1382 TCCGGA T^CCGGA
AcsI 787 GAATTT [GA]^AATT[CT]
AcyI 1285 GGCGTC G[GA]^CG[CT]C
AocI 201 CCTGAGG CC^T[ACGT]AGG
ApoI 787 GAATTT [GA]^AATT[CT]
AspI 34 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1343 CCCGGG C^[CT]CG[GA]G
AviII 1500 TGCGCA TGC^GCA
BanII 824 GAGCCC G[GA]GC[CT]^C
BbvCI 1481 CCTCAGC CCTCAGC(-5/-2)
BsaHI 1285 GGCGTC G[GA]^CG[CT]C
BsaWI 1382 TCCGGA [AT]^CCGG[AT]
BseAI 1382 TCCGGA T^CCGGA
BsiEI 33 CGACCG CG[GA][CT]^CG
BsmFI 52 GTCCC GGGAC(10/14)
BsoBI 1343 CCCGGG C^[CT]CG[GA]G
BspEI 1382 TCCGGA T^CCGGA
BspMI 674 ACCTGC ACCTGC(4/8)
BsrDI 707 GCAATG GCAATG(2/0)
BstXI 837 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1379 AGATCC [GA]^GATC[CT]
Bsu36I 201 CCTGAGG CC^T[ACGT]AGG
DraII 1253 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eco81I 201 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1253 AGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 652 CCCGC CCCGC(4/6)
FspI 1500 TGCGCA TGC^GCA
HaeII 11 GGCGCT [GA]GCGC^[CT]
Hin1I 1285 GGCGTC G[GA]^CG[CT]C
MflI 1379 AGATCC [GA]^GATC[CT]
MroI 1382 TCCGGA T^CCGGA
NdeI 1098 CATATG CA^TATG
NlaIV 645 GGAACC GG[ACGT]^[ACGT]CC
NspI 1494 ACATGC [GA]CATG^[CT]
PflFI 34 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 776 CTGCAG CTGCA^G
SapI 864 GAAGAGC GCTCTTC(1/4)
SmaI 1343 CCCGGG CCC^GGG
SmlI 1249 CTCAAG C^T[CT][GA]AG
SspI 494 AATATT AAT^ATT
TfiI 488 GAATC G^A[AT]TC
TspRI 741 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 34 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1379 AGATCC [GA]^GATC[CT]
XmaCI 1343 CCCGGG C^CCGGG
XmaI 1343 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 37 CGCG CG^CG
1400 CGCG
AfaI 580 GTAC GT^AC
858 GTAC
AluI 48 AGCT AG^CT
942 AGCT
AseI 400 ATTAAT AT^TAAT
1417 ATTAAT
AsnI 400 ATTAAT AT^TAAT
1417 ATTAAT
AvaII 661 GGACC G^G[AT]CC
1410 GGACC
BfaI 311 CTAG C^TAG
922 CTAG
BsmAI 526 GAGAC GTCTC(1/5)
1315 GAGAC
BsmI 442 GAATGC GAATGC(1/-1)
1427 GAATGC
Bsp1407I 579 TGTACA T^GTACA
857 TGTACA
BspHI 438 TCATGA T^CATGA
971 TCATGA
BsrGI 579 TGTACA T^GTACA
857 TGTACA
BsrI 153 ACTGG ACTGG(1/-1)
1010 ACTGG
BstUI 37 CGCG CG^CG
1400 CGCG
Cac8I 446 GCAGGC GC[ACGT]^[ACGT]GC
1301 GCAAGC
DraI 123 TTTAAA TTT^AAA
393 TTTAAA
EarI 157 GAAGAG CTCTTC(1/4)
864 GAAGAG
EcoT14I 719 CCTTGG C^C[AT][AT]GG
1515 CCAAGG
HgaI 38 GCGTC GACGC(5/10)
1286 GCGTC
Ksp632I 157 GAAGAG CTCTTC(1/4)
864 GAAGAG
MaeI 311 CTAG C^TAG
922 CTAG
MvnI 37 CGCG CG^CG
1400 CGCG
PleI 1245 GACTC GAGTC(4/5)
1260 GACTC
PshBI 400 ATTAAT AT^TAAT
1417 ATTAAT
RcaI 438 TCATGA T^CATGA
971 TCATGA
RsaI 580 GTAC GT^AC
858 GTAC
SspBI 579 TGTACA T^GTACA
857 TGTACA
StyI 719 CCTTGG C^C[AT][AT]GG
1515 CCAAGG
XcmI 1000 CCACACCCCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1187 CCATTGGGAAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AlwI AspHI BbsI BbvI
BcnI BmyI BpuAI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstF5I
CfoI Cfr13I DdeI DpnII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MslI
MspI MwoI NciI NdeII NlaIII
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI Tru9I TseI Tsp45I Tsp509I
TthHB8I