Restriction map for
pF1KB4998
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
BalI BamHI BanI BanII BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HindIII
HpaI HphI KasI KpnI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuII RcaI RsrII SacI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 474 GTCGAC GT^[AC][GT]AC
AflIII 125 ACGTGT A^C[GA][CT]GT
AvrII 364 CCTAGG C^CTAGG
BbsI 97 GTCTTC GAAGAC(2/6)
BfaI 365 CTAG C^TAG
BglII 532 AGATCT A^GATCT
BlnI 364 CCTAGG C^CTAGG
BpmI 528 CTCCAG CTGGAG(16/14)
BpuAI 97 GTCTTC GAAGAC(2/6)
Bsp1407I 128 TGTACA T^GTACA
BspMI 323 GCAGGT ACCTGC(4/8)
BsrGI 128 TGTACA T^GTACA
BsrI 580 CCAGT ACTGG(1/-1)
BssHII 268 GCGCGC G^CGCGC
DraII 550 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 477 GACCTCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 550 AGGTCCC [GA]G^G[ACGT]CC[CT]
HincII 474 GTCGAC GT[CT]^[GA]AC
HindII 474 GTCGAC GT[CT]^[GA]AC
MaeI 365 CTAG C^TAG
MaeII 125 ACGT A^CGT
NcoI 221 CCATGG C^CATGG
NlaIII 222 CATG CATG^
NruI 610 TCGCGA TCG^CGA
PpuMI 550 AGGTCCC [GA]G^G[AT]CC[CT]
PvuI 613 CGATCG CGAT^CG
SalI 474 GTCGAC G^TCGAC
SfaNI 217 GATGC GCATC(5/9)
SfcI 429 CTACAG C^T[GA][CT]AG
SmlI 46 CTCAAG C^T[CT][GA]AG
SspBI 128 TGTACA T^GTACA
TfiI 595 GAATC G^A[AT]TC
TspRI 581 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcyI 347 GACGCC G[GA]^CG[CT]C
371 GGCGTC
AluI 4 AGCT AG^CT
251 AGCT
BbvCI 87 GCTGAGG CCTCAGC(-5/-2)
202 GCTGAGG
BsaHI 347 GACGCC G[GA]^CG[CT]C
371 GGCGTC
BsrBI 435 CCGCTC CCGCTC(-3/-3)
650 GAGCGG
BstF5I 132 CATCC GGATG(2/0)
216 GGATG
BstYI 490 AGATCC [GA]^GATC[CT]
532 AGATCT
Cac8I 248 GCGAGC GC[ACGT]^[ACGT]GC
268 GCGCGC
DdeI 88 CTGAG C^T[ACGT]AG
203 CTGAG
EarI 618 GAAGAG CTCTTC(1/4)
640 GAAGAG
FokI 132 CATCC GGATG(9/13)
216 GGATG
Hin1I 347 GACGCC G[GA]^CG[CT]C
371 GGCGTC
Ksp632I 618 GAAGAG CTCTTC(1/4)
640 GAAGAG
KspI 136 CCGCGG CCGC^GG
162 CCGCGG
MflI 490 AGATCC [GA]^GATC[CT]
532 AGATCT
MspA1I 136 CCGCGG C[AC]G^C[GT]G
162 CCGCGG
SacII 136 CCGCGG CCGC^GG
162 CCGCGG
XhoII 490 AGATCC [GA]^GATC[CT]
532 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AfaI AlwI AvaII
BbvI BcnI BsaI BsaJI BsiEI
BsiYI BslI BsmAI BsmFI BssKI
BstNI BstUI CfoI Cfr13I DpnII
DsaI EaeI EagI EclXI Eco52I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI MboI MboII MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI StyI TaqI TseI
TthHB8I