Restriction map for
pF1KB5014
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BanII
BbeI BbrPI BcgI BciVI BfaI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BspLU11I BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 61 GTCTAC GT^[AC][GT]AC
AcsI 142 AAATTC [GA]^AATT[CT]
AflII 1099 CTTAAG C^TTAAG
AocI 697 CCTGAGG CC^T[ACGT]AGG
ApoI 142 AAATTC [GA]^AATT[CT]
BbvCI 1015 GCTGAGG CCTCAGC(-5/-2)
BclI 467 TGATCA T^GATCA
BfrI 1099 CTTAAG C^TTAAG
BglI 29 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 858 GGGCAC G[AGT]GC[ACT]^C
BsaWI 645 ACCGGA [AT]^CCGG[AT]
BsgI 277 GTGCAG GTGCAG(16/14)
Bsp1286I 858 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 512 TGTACA T^GTACA
BspDI 673 ATCGAT AT^CGAT
BspMI 957 GCAGGT ACCTGC(4/8)
BsrBI 661 GAGCGG CCGCTC(-3/-3)
BsrGI 512 TGTACA T^GTACA
Bsu36I 697 CCTGAGG CC^T[ACGT]AGG
ClaI 673 ATCGAT AT^CGAT
EarI 763 GAAGAG CTCTTC(1/4)
Eco81I 697 CCTGAGG CC^T[ACGT]AGG
EcoNI 951 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 467 TGATCA T^GATCA
HgaI 267 GACGC GACGC(5/10)
Ksp632I 763 GAAGAG CTCTTC(1/4)
MseI 1100 TTAA T^TAA
NspI 362 ACATGC [GA]CATG^[CT]
PflMI 264 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfiI 28 GGCCTCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1099 CTTAAG C^T[CT][GA]AG
SspBI 512 TGTACA T^GTACA
Tru9I 1100 TTAA T^TAA
Tsp45I 173 GTGAC ^GT[GC]AC
Van91I 264 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 513 GTAC GT^AC
810 GTAC
AluI 332 AGCT AG^CT
1247 AGCT
AlwNI 76 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
265 CAGACGCTG
BamHI 627 GGATCC G^GATCC
866 GGATCC
BanI 418 GGCACC G^G[CT][GA]CC
970 GGCACC
BcnI 830 CCCGG CC^[GC]GG
1228 CCCGG
BsmBI 89 GAGACG CGTCTC(1/5)
1033 GAGACG
BstYI 627 GGATCC [GA]^GATC[CT]
866 GGATCC
EcoT14I 584 CCTTGG C^C[AT][AT]GG
1238 CCAAGG
FauI 104 CCCGC CCCGC(4/6)
663 GCGGG
MaeII 461 ACGT A^CGT
1036 ACGT
MaeIII 173 GTGAC ^GT[ACGT]AC
1045 GTAAC
MflI 627 GGATCC [GA]^GATC[CT]
866 GGATCC
MspA1I 16 CCGCTG C[AC]G^C[GT]G
105 CCGCTG
NciI 830 CCCGG CC^[GC]GG
1228 CCCGG
RsaI 513 GTAC GT^AC
810 GTAC
StyI 584 CCTTGG C^C[AT][AT]GG
1238 CCAAGG
TfiI 115 GAATC G^A[AT]TC
795 GATTC
Tsp509I 143 AATT ^AATT
879 AATT
TspRI 518 CACTG CA[GC]TG[ACGT][ACGT]^
791 CAGTG
XhoII 627 GGATCC [GA]^GATC[CT]
866 GGATCC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AlwI AvaI AvaII BbsI
BbvI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TthHB8I