Restriction map for
pF1KB5026
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FauI FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SwaI Tsp45I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 538 GTCTAC GT^[AC][GT]AC
AccII 81 CGCG CG^CG
AcyI 969 GGCGCC G[GA]^CG[CT]C
AflIII 771 ACATGT A^C[GA][CT]GT
Alw44I 97 GTGCAC G^TGCAC
ApaI 1336 GGGCCC GGGCC^C
ApaLI 97 GTGCAC G^TGCAC
Asp700I 1001 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 97 GTGCAC G[AT]GC[AT]^C
AvaII 379 GGACC G^G[AT]CC
BalI 474 TGGCCA TGG^CCA
BanI 969 GGCGCC G^G[CT][GA]CC
BanII 1336 GGGCCC G[GA]GC[CT]^C
BbeI 969 GGCGCC GGCGC^C
BbsI 441 GTCTTC GAAGAC(2/6)
BbvI 395 GCAGC GCAGC(8/12)
BpuAI 441 GTCTTC GAAGAC(2/6)
BsaBI 196 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 969 GGCGCC G[GA]^CG[CT]C
BseRI 1315 GAGGAG GAGGAG(10/8)
BsgI 1258 GTGCAG GTGCAG(16/14)
BsiHKAI 97 GTGCAC G[AT]GC[AT]^C
BsmBI 845 GAGACG CGTCTC(1/5)
BspLU11I 771 ACATGT A^CATGT
BsrDI 238 GCAATG GCAATG(2/0)
BstUI 81 CGCG CG^CG
BstXI 260 CCACAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 828 TTTAAA TTT^AAA
DraIII 1188 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 474 TGGCCA [CT]^GGCC[GA]
EarI 118 CTCTTC CTCTTC(1/4)
EcoRV 500 GATATC GAT^ATC
Hin1I 969 GGCGCC G[GA]^CG[CT]C
KasI 969 GGCGCC G^GCGCC
Ksp632I 118 CTCTTC CTCTTC(1/4)
MaeIII 114 GTTAC ^GT[ACGT]AC
MamI 196 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 474 TGGCCA TGG^CCA
MscI 474 TGGCCA TGG^CCA
MspA1I 919 CAGCTG C[AC]G^C[GT]G
MvnI 81 CGCG CG^CG
NarI 969 GGCGCC GG^CGCC
NcoI 32 CCATGG C^CATGG
NheI 9 GCTAGC G^CTAGC
NspI 771 ACATGT [GA]CATG^[CT]
PvuII 919 CAGCTG CAG^CTG
SexAI 41 ACCAGGT A^CC[AT]GGT
SfaNI 59 GCATC GCATC(5/9)
SfoI 969 GGCGCC GGC^GCC
SspI 570 AATATT AAT^ATT
StuI 807 AGGCCT AGG^CCT
TaqI 204 TCGA T^CGA
TseI 395 GCAGC G^C[AT]GC
TthHB8I 204 TCGA T^CGA
XmnI 1001 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 719 GTAC GT^AC
1177 GTAC
AvaI 371 CTCGGG C^[CT]CG[GA]G
558 CCCGAG
BcnI 104 CCGGG CC^[GC]GG
1305 CCGGG
BmyI 97 GTGCAC G[AGT]GC[ACT]^C
1336 GGGCCC
BsmAI 845 GAGAC GTCTC(1/5)
406 GTCTC
BsmFI 506 GGGAC GGGAC(10/14)
556 GTCCC
BsoBI 371 CTCGGG C^[CT]CG[GA]G
558 CCCGAG
Bsp1286I 97 GTGCAC G[AGT]GC[ACT]^C
1336 GGGCCC
Bsp1407I 718 TGTACA T^GTACA
1176 TGTACA
BsrGI 718 TGTACA T^GTACA
1176 TGTACA
DraII 1335 GGGGCCC [GA]G^G[ACGT]CC[CT]
1336 GGGCCCC
DsaI 32 CCATGG C^C[GA][CT]GG
1283 CCGTGG
EcoO109I 1335 GGGGCCC [GA]G^G[ACGT]CC[CT]
1336 GGGCCCC
HaeII 969 GGCGCC [GA]GCGC^[CT]
1308 GGCGCT
MaeII 848 ACGT A^CGT
943 ACGT
NciI 104 CCGGG CC^[GC]GG
1305 CCGGG
PflMI 42 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
70 CCAAATAGTGG
PstI 304 CTGCAG CTGCA^G
1114 CTGCAG
RsaI 719 GTAC GT^AC
1177 GTAC
SspBI 718 TGTACA T^GTACA
1176 TGTACA
Van91I 42 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
70 CCAAATAGTGG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI BfaI BpmI BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfcI StyI TfiI Tru9I Tsp509I
TspRI XhoII