Restriction map for
pF1KB5089
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI BbrPI BbsI BfrI BglII
BpuAI BsaBI BsiWI Bsp1407I BspDI
BspHI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eco47III EcoRI EcoRV EcoT22I FseI
HindIII HpaI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 2090 GGTACC G^GTACC
AocI 1111 CCTGAGG CC^T[ACGT]AGG
Asp718I 2090 GGTACC G^GTACC
AspI 1029 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3033 CCTAGG C^CTAGG
BclI 2576 TGATCA T^GATCA
BfaI 3034 CTAG C^TAG
BlnI 3033 CCTAGG C^CTAGG
BlpI 1929 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1929 GCTCAGC GC^T[ACGT]AGC
BsaAI 2236 TACGTG [CT]AC^GT[GA]
BsmI 1752 GAATGC GAATGC(1/-1)
BspLU11I 2249 ACATGT A^CATGT
BstEII 120 GGTGACC G^GT[ACGT]ACC
BstPI 120 GGTGACC G^GT[ACGT]ACC
Bsu36I 1111 CCTGAGG CC^T[ACGT]AGG
CelII 1929 GCTCAGC GC^T[ACGT]AGC
DraIII 965 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 312 GACGGTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1111 CCTGAGG CC^T[ACGT]AGG
EcoO65I 120 GGTGACC G^GT[ACGT]ACC
FbaI 2576 TGATCA T^GATCA
KpnI 2090 GGTACC GGTAC^C
MaeI 3034 CTAG C^TAG
PflFI 1029 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 383 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 1280 AGTACT AGT^ACT
SexAI 116 ACCTGGT A^CC[AT]GGT
SfiI 3507 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3640 CCCGGG CCC^GGG
StuI 1551 AGGCCT AGG^CCT
TfiI 571 GAATC G^A[AT]TC
Tth111I 1029 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 383 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3640 CCCGGG C^CCGGG
XmaI 3640 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 2452 TCCGGA T^CCGGA
2897 TCCGGA
AcsI 586 AAATTC [GA]^AATT[CT]
2410 AAATTC
AflIII 1091 ACGTGT A^C[GA][CT]GT
2249 ACATGT
ApaI 165 GGGCCC GGGCC^C
2615 GGGCCC
ApoI 586 AAATTC [GA]^AATT[CT]
2410 AAATTC
AvaI 768 CCCGAG C^[CT]CG[GA]G
3640 CCCGGG
AviII 458 TGCGCA TGC^GCA
2930 TGCGCA
BalI 2171 TGGCCA TGG^CCA
2661 TGGCCA
BamHI 506 GGATCC G^GATCC
2426 GGATCC
BbeI 7 GGCGCC GGCGC^C
700 GGCGCC
BbvCI 2793 CCTCAGC CCTCAGC(-5/-2)
3001 GCTGAGG
BcgI 1485 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1746 CGAGGTGAATGC
BciVI 2455 GGATAC GTATCC(6/5)
1385 GTATCC
BseAI 2452 TCCGGA T^CCGGA
2897 TCCGGA
BsgI 367 GTGCAG GTGCAG(16/14)
3115 GTGCAG
BsmBI 1301 GAGACG CGTCTC(1/5)
2668 GAGACG
BsoBI 768 CCCGAG C^[CT]CG[GA]G
3640 CCCGGG
BspEI 2452 TCCGGA T^CCGGA
2897 TCCGGA
BstAPI 3117 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3465 GCAGGTCCTGC
FspI 458 TGCGCA TGC^GCA
2930 TGCGCA
HincII 1780 GTCAAC GT[CT]^[GA]AC
3397 GTCAAC
HindII 1780 GTCAAC GT[CT]^[GA]AC
3397 GTCAAC
KasI 7 GGCGCC G^GCGCC
700 GGCGCC
MluNI 2171 TGGCCA TGG^CCA
2661 TGGCCA
MroI 2452 TCCGGA T^CCGGA
2897 TCCGGA
MscI 2171 TGGCCA TGG^CCA
2661 TGGCCA
MseI 2588 TTAA T^TAA
3371 TTAA
NarI 7 GGCGCC GG^CGCC
700 GGCGCC
NspI 1976 ACATGC [GA]CATG^[CT]
2249 ACATGT
SacI 1488 GAGCTC GAGCT^C
2866 GAGCTC
SapI 2332 GAAGAGC GCTCTTC(1/4)
1986 GCTCTTC
SfoI 7 GGCGCC GGC^GCC
700 GGCGCC
SmlI 1579 CTTGAG C^T[CT][GA]AG
2098 CTCAAG
Tru9I 2588 TTAA T^TAA
3371 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI Asp700I AspEI
AspHI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI