Restriction map for
pF1KB5090
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII AccI AccII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BanII BbeI BbrPI BcgI
BciVI BfrI BlnI BlpI Bpu1102I
BsaBI BsiWI BsmBI BsoBI BspDI
BspHI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoNI EcoO65I
EcoRV FseI FspI HindIII HpaI
KasI KspI MamI MluI MluNI
MscI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 38 GGTACC G^GTACC
AccIII 452 TCCGGA T^CCGGA
AflIII 1940 ACATGT A^C[GA][CT]GT
AocI 1236 CCTGAGG CC^T[ACGT]AGG
Asp718I 38 GGTACC G^GTACC
AspHI 1794 GAGCAC G[AT]GC[AT]^C
BamHI 1138 GGATCC G^GATCC
BbsI 2115 GAAGAC GAAGAC(2/6)
BbvCI 2234 CCTCAGC CCTCAGC(-5/-2)
BclI 1313 TGATCA T^GATCA
BglI 358 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 558 AGATCT A^GATCT
BpuAI 2115 GAAGAC GAAGAC(2/6)
BsaAI 688 TACGTG [CT]AC^GT[GA]
BsaWI 452 TCCGGA [AT]^CCGG[AT]
BseAI 452 TCCGGA T^CCGGA
BsiEI 904 CGGCCG CG[GA][CT]^CG
BsiHKAI 1794 GAGCAC G[AT]GC[AT]^C
BsmI 2044 GCATTC GAATGC(1/-1)
Bsp1407I 894 TGTACA T^GTACA
BspEI 452 TCCGGA T^CCGGA
BspLU11I 1940 ACATGT A^CATGT
BsrGI 894 TGTACA T^GTACA
Bsu36I 1236 CCTGAGG CC^T[ACGT]AGG
EagI 904 CGGCCG C^GGCCG
EclXI 904 CGGCCG C^GGCCG
Eco52I 904 CGGCCG C^GGCCG
Eco81I 1236 CCTGAGG CC^T[ACGT]AGG
EcoRI 1219 GAATTC G^AATTC
EcoT22I 991 ATGCAT ATGCA^T
FbaI 1313 TGATCA T^GATCA
HaeII 2098 GGCGCT [GA]GCGC^[CT]
KpnI 38 GGTACC GGTAC^C
MroI 452 TCCGGA T^CCGGA
NdeI 131 CATATG CA^TATG
NsiI 991 ATGCAT ATGCA^T
PshAI 1020 GACCGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1602 ACCTGGT A^CC[AT]GGT
SphI 983 GCATGC GCATG^C
SspBI 894 TGTACA T^GTACA
StuI 1344 AGGCCT AGG^CCT
XbaI 471 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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BmyI 1161 GGGCAC G[AGT]GC[ACT]^C
1794 GAGCAC
BsaI 1324 GAGACC GGTCTC(1/5)
1363 GAGACC
Bsp1286I 1161 GGGCAC G[AGT]GC[ACT]^C
1794 GAGCAC
BstXI 1411 CCAAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1988 CCAATATCCTGG
DsaI 404 CCGTGG C^C[GA][CT]GG
1907 CCGTGG
EarI 456 GAAGAG CTCTTC(1/4)
729 GAAGAG
HincII 833 GTCAAC GT[CT]^[GA]AC
2277 GTTGAC
HindII 833 GTCAAC GT[CT]^[GA]AC
2277 GTTGAC
Ksp632I 456 GAAGAG CTCTTC(1/4)
729 GAAGAG
MfeI 1272 CAATTG C^AATTG
1490 CAATTG
MunI 1272 CAATTG C^AATTG
1490 CAATTG
PflMI 1427 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1902 CCAGTCCGTGG
TaqI 223 TCGA T^CGA
1565 TCGA
TthHB8I 223 TCGA T^CGA
1565 TCGA
Van91I 1427 CCATCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1902 CCAGTCCGTGG
XcmI 395 CCACAGCAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1489 CCAATTGGAACCTGG
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AvaII BanI
BbvI BcnI BfaI BpmI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII