Restriction map for
pF1KB5096
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BbrPI BbvCI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaWI BseAI BsgI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FspI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspI
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SbfI
SexAI SfiI SgrAI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI XbaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Alw44I 712 GTGCAC G^TGCAC
AlwNI 1465 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1503 CCTGAGG CC^T[ACGT]AGG
ApaI 943 GGGCCC GGGCC^C
ApaLI 712 GTGCAC G^TGCAC
AspI 1738 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1373 GGATCC G^GATCC
BbeI 266 GGCGCC GGCGC^C
BciVI 1054 GGATAC GTATCC(6/5)
BclI 1571 TGATCA T^GATCA
BglI 763 GCCATCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 149 TACGTA [CT]AC^GT[GA]
BsaI 346 GGTCTC GGTCTC(1/5)
BsiEI 1537 CGGCCG CG[GA][CT]^CG
BsmBI 1561 GAGACG CGTCTC(1/5)
BsmI 744 GCATTC GAATGC(1/-1)
BspHI 530 TCATGA T^CATGA
BspMI 131 ACCTGC ACCTGC(4/8)
BsrFI 1535 GCCGGC [GA]^CCGG[CT]
BssSI 715 CACGAG CACGAG(-5/-1)
BstBI 320 TTCGAA TT^CGAA
Bsu36I 1503 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1535 GCCGGC [GA]^CCGG[CT]
EagI 1537 CGGCCG C^GGCCG
EclXI 1537 CGGCCG C^GGCCG
Eco52I 1537 CGGCCG C^GGCCG
Eco81I 1503 CCTGAGG CC^T[ACGT]AGG
EcoNI 1112 CCTACGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1571 TGATCA T^GATCA
FseI 1534 GGCCGGCC GGCCGG^CC
KasI 266 GGCGCC G^GCGCC
MaeII 150 ACGT A^CGT
MseI 1208 TTAA T^TAA
MspA1I 185 CAGCGG C[AC]G^C[GT]G
NaeI 1535 GCCGGC GCC^GGC
NarI 266 GGCGCC GG^CGCC
NgoMIV 1535 GCCGGC G^CCGGC
NspV 320 TTCGAA TT^CGAA
PaeR7I 739 CTCGAG C^TCGAG
PflFI 1738 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 530 TCATGA T^CATGA
SacI 1321 GAGCTC GAGCT^C
SapI 1318 GAAGAGC GCTCTTC(1/4)
ScaI 1301 AGTACT AGT^ACT
SfcI 115 CTACAG C^T[GA][CT]AG
SfoI 266 GGCGCC GGC^GCC
SfuI 320 TTCGAA TT^CGAA
SmaI 139 CCCGGG CCC^GGG
SmlI 739 CTCGAG C^T[CT][GA]AG
SnaBI 149 TACGTA TAC^GTA
Tru9I 1208 TTAA T^TAA
Tth111I 1738 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 739 CTCGAG C^TCGAG
XmaCI 139 CCCGGG C^CCGGG
XmaI 139 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 712 GTGCAC G[AT]GC[AT]^C
1321 GAGCTC
AvaI 139 CCCGGG C^[CT]CG[GA]G
739 CTCGAG
AvaII 144 GGACC G^G[AT]CC
1697 GGACC
BalI 977 TGGCCA TGG^CCA
1071 TGGCCA
BcgI 56 GCACCGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
566 GCACGCCCTTCG
BsiHKAI 712 GTGCAC G[AT]GC[AT]^C
1321 GAGCTC
BsoBI 139 CCCGGG C^[CT]CG[GA]G
739 CTCGAG
BstYI 678 GGATCT [GA]^GATC[CT]
1373 GGATCC
EarI 1255 CTCTTC CTCTTC(1/4)
1318 GAAGAG
Ksp632I 1255 CTCTTC CTCTTC(1/4)
1318 GAAGAG
MflI 678 GGATCT [GA]^GATC[CT]
1373 GGATCC
MluNI 977 TGGCCA TGG^CCA
1071 TGGCCA
MscI 977 TGGCCA TGG^CCA
1071 TGGCCA
NcoI 555 CCATGG C^CATGG
1692 CCATGG
PpuMI 143 GGGACCT [GA]G^G[AT]CC[CT]
1696 GGGACCC
XcmI 837 CCAGCAACTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1041 CCAAGTGATGGATGG
XhoII 678 GGATCT [GA]^GATC[CT]
1373 GGATCC
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI Aor51HI ApoI BanI
BanII BbsI BbvI BcnI BmyI
BpuAI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco47III EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I