Restriction map for
pF1KB5098
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# NO CUT ENZYMES ( 68 enzymes) #
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AatII Acc65I AclI AcsI AflII
AhdI Aor51HI ApaI ApoI AscI
Asp718I AspEI BclI BfrI BglI
BsaBI BsiWI BsmBI BspDI BspLU11I
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI DraI DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PmeI PshAI Psp1406I PvuI
SalI SapI SbfI ScaI SfiI
SfuI SnaBI SpeI Sse8387I SspI
SwaI Tsp509I XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1633 GTCTAC GT^[AC][GT]AC
AccIII 258 TCCGGA T^CCGGA
AflIII 1953 ACGTGT A^C[GA][CT]GT
AgeI 1261 ACCGGT A^CCGGT
Alw44I 2710 GTGCAC G^TGCAC
ApaLI 2710 GTGCAC G^TGCAC
AseI 2788 ATTAAT AT^TAAT
AsnI 2788 ATTAAT AT^TAAT
Asp700I 2259 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2392 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1958 TGCGCA TGC^GCA
AvrII 3129 CCTAGG C^CTAGG
BamHI 1868 GGATCC G^GATCC
BbrPI 1952 CACGTG CAC^GTG
BbsI 2802 GAAGAC GAAGAC(2/6)
BbvCI 2134 GCTGAGG CCTCAGC(-5/-2)
BlnI 3129 CCTAGG C^CTAGG
BpuAI 2802 GAAGAC GAAGAC(2/6)
BseAI 258 TCCGGA T^CCGGA
BsgI 1969 GTGCAG GTGCAG(16/14)
BsmI 3424 GCATTC GAATGC(1/-1)
Bsp1407I 1460 TGTACA T^GTACA
BspEI 258 TCCGGA T^CCGGA
BsrGI 1460 TGTACA T^GTACA
BssSI 1781 CACGAG CACGAG(-5/-1)
CpoI 2560 CGGACCG CG^G[AT]CCG
EcoNI 1965 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1958 TGCGCA TGC^GCA
MroI 258 TCCGGA T^CCGGA
NaeI 1244 GCCGGC GCC^GGC
NgoMIV 1244 GCCGGC G^CCGGC
PaeR7I 112 CTCGAG C^TCGAG
PflFI 2392 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1221 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1261 ACCGGT A^CCGGT
PmaCI 1952 CACGTG CAC^GTG
PmlI 1952 CACGTG CAC^GTG
PshBI 2788 ATTAAT AT^TAAT
PstI 3052 CTGCAG CTGCA^G
RsrII 2560 CGGACCG CG^G[AT]CCG
SexAI 1988 ACCTGGT A^CC[AT]GGT
SgrAI 2517 CACCGGCG C[GA]^CCGG[CT]G
SphI 1595 GCATGC GCATG^C
SspBI 1460 TGTACA T^GTACA
StuI 1785 AGGCCT AGG^CCT
Tth111I 2392 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1221 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 112 CTCGAG C^TCGAG
XmnI 2259 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 1918 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
2347 CAGCTGCTG
AocI 406 CCTAAGG CC^T[ACGT]AGG
820 CCTGAGG
BcgI 1305 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2525 GCATGGGCATCG
BciVI 2218 GTATCC GTATCC(6/5)
2305 GTATCC
BglII 1793 AGATCT A^GATCT
1808 AGATCT
BlpI 27 GCTCAGC GC^T[ACGT]AGC
982 GCTGAGC
Bpu1102I 27 GCTCAGC GC^T[ACGT]AGC
982 GCTGAGC
BsaAI 916 TACGTG [CT]AC^GT[GA]
1952 CACGTG
BspHI 1718 TCATGA T^CATGA
2643 TCATGA
BsrBI 799 GAGCGG CCGCTC(-3/-3)
2296 GAGCGG
BstAPI 75 GCAGACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1586 GCAGCCTGTGC
Bsu36I 406 CCTAAGG CC^T[ACGT]AGG
820 CCTGAGG
CelII 27 GCTCAGC GC^T[ACGT]AGC
982 GCTGAGC
Eco81I 406 CCTAAGG CC^T[ACGT]AGG
820 CCTGAGG
HincII 2536 GTCAAC GT[CT]^[GA]AC
2905 GTTGAC
HindII 2536 GTCAAC GT[CT]^[GA]AC
2905 GTTGAC
MseI 2723 TTAA T^TAA
2789 TTAA
RcaI 1718 TCATGA T^CATGA
2643 TCATGA
SfcI 1635 CTACAG C^T[GA][CT]AG
3052 CTGCAG
SmlI 112 CTCGAG C^T[CT][GA]AG
1789 CTCAAG
TfiI 621 GAATC G^A[AT]TC
2831 GAATC
Tru9I 2723 TTAA T^TAA
2789 TTAA
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# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfoI SmaI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII XmaCI XmaI