Restriction map for
pF1KB5104
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FauI FseI
FspI HaeII HhaI HinP1I KasI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 1015 TCCGGA T^CCGGA
AcyI 1625 GGCGTC G[GA]^CG[CT]C
AflIII 664 ACACGT A^C[GA][CT]GT
AseI 1201 ATTAAT AT^TAAT
AsnI 1201 ATTAAT AT^TAAT
AspHI 65 GAGCTC G[AT]GC[AT]^C
BbsI 1081 GAAGAC GAAGAC(2/6)
BbvCI 415 GCTGAGG CCTCAGC(-5/-2)
BclI 407 TGATCA T^GATCA
BpuAI 1081 GAAGAC GAAGAC(2/6)
BsaHI 1625 GGCGTC G[GA]^CG[CT]C
BsaJI 935 CCATGG C^C[ACGT][ACGT]GG
BsaWI 1015 TCCGGA [AT]^CCGG[AT]
BseAI 1015 TCCGGA T^CCGGA
BsiHKAI 65 GAGCTC G[AT]GC[AT]^C
BsiYI 584 CCATATTTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 584 CCATATTTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1539 GAGAC GTCTC(1/5)
BspEI 1015 TCCGGA T^CCGGA
DraI 438 TTTAAA TTT^AAA
DraII 592 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 935 CCATGG C^C[GA][CT]GG
EcoO109I 592 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 571 GATATC GAT^ATC
EcoT14I 935 CCATGG C^C[AT][AT]GG
FbaI 407 TGATCA T^GATCA
Hin1I 1625 GGCGTC G[GA]^CG[CT]C
HincII 1251 GTTAAC GT[CT]^[GA]AC
HindII 1251 GTTAAC GT[CT]^[GA]AC
HindIII 1235 AAGCTT A^AGCTT
HpaI 1251 GTTAAC GTT^AAC
HphI 1303 TCACC GGTGA(8/7)
MaeII 666 ACGT A^CGT
MroI 1015 TCCGGA T^CCGGA
NcoI 935 CCATGG C^CATGG
NdeI 133 CATATG CA^TATG
NspI 207 ACATGC [GA]CATG^[CT]
PpuMI 592 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1201 ATTAAT AT^TAAT
SacI 65 GAGCTC GAGCT^C
SfcI 1174 CTACAG C^T[GA][CT]AG
SpeI 985 ACTAGT A^CTAGT
StuI 756 AGGCCT AGG^CCT
StyI 935 CCATGG C^C[AT][AT]GG
XcmI 1343 CCATAAACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 72 GTAC GT^AC
1286 GTAC
AlwNI 289 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
292 CAGCTGCTG
BanI 99 GGCACC G^G[CT][GA]CC
1356 GGTGCC
BanII 65 GAGCTC G[GA]GC[CT]^C
991 GAGCCC
BpmI 1585 CTCCAG CTGGAG(16/14)
1307 CTGGAG
BsmFI 215 GGGAC GGGAC(10/14)
678 GTCCC
BsmI 347 GAATGC GAATGC(1/-1)
447 GAATGC
BssKI 942 CCTGG ^CC[ACGT]GG
1306 CCTGG
BstAPI 291 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
730 GCAGAATGTGC
BstNI 942 CCTGG CC^[AT]GG
1306 CCTGG
BstYI 360 AGATCC [GA]^GATC[CT]
720 GGATCT
Cac8I 1038 GCCAGC GC[ACGT]^[ACGT]GC
1338 GCAAGC
EarI 334 GAAGAG CTCTTC(1/4)
1576 GAAGAG
EcoRI 421 GAATTC G^AATTC
655 GAATTC
EcoRII 942 CCTGG ^CC[AT]GG
1306 CCTGG
HapII 522 CCGG C^CGG
1016 CCGG
HgaI 45 GCGTC GACGC(5/10)
1626 GCGTC
HpaII 522 CCGG C^CGG
1016 CCGG
Ksp632I 334 GAAGAG CTCTTC(1/4)
1576 GAAGAG
MflI 360 AGATCC [GA]^GATC[CT]
720 GGATCT
MspI 522 CCGG C^CGG
1016 CCGG
MvaI 942 CCTGG CC^[AT]GG
1306 CCTGG
RsaI 72 GTAC GT^AC
1286 GTAC
ScrFI 942 CCTGG CC^[ACGT]GG
1306 CCTGG
SmlI 1126 CTCAAG C^T[CT][GA]AG
1266 CTCAAG
TfiI 1496 GATTC G^A[AT]TC
1533 GATTC
XhoII 360 AGATCC [GA]^GATC[CT]
720 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
AvaII BbvI BfaI BmyI Bsp1286I
BsrI BstF5I BstXI Cfr13I DdeI
DpnII Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MwoI
NdeII NlaIII NlaIV PleI PvuII
Sau3AI Sau96I SfaNI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI