Restriction map for
pF1KB5108
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BpmI BsaAI BsaHI BseAI
BseRI BsiEI BsmBI BspDI BspEI
BspLU11I BspMI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI Hin1I
HindIII KasI KspI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 20 GGTACC G^GTACC
AgeI 259 ACCGGT A^CCGGT
ApaI 137 GGGCCC GGGCC^C
Asp718I 20 GGTACC G^GTACC
AvaI 113 CCCGAG C^[CT]CG[GA]G
AvaII 1237 GGACC G^G[AT]CC
BanI 20 GGTACC G^G[CT][GA]CC
BbsI 571 GAAGAC GAAGAC(2/6)
BpuAI 571 GAAGAC GAAGAC(2/6)
BsaBI 1312 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 116 GAGACC GGTCTC(1/5)
BsaWI 259 ACCGGT [AT]^CCGG[AT]
BsiWI 60 CGTACG C^GTACG
BsmI 2356 GAATGC GAATGC(1/-1)
BsoBI 113 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2277 TGTACA T^GTACA
BspHI 2024 TCATGA T^CATGA
BsrBI 6 GAGCGG CCGCTC(-3/-3)
BsrGI 2277 TGTACA T^GTACA
BssSI 49 CACGAG CACGAG(-5/-1)
BstAPI 625 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1710 GGATG GGATG(2/0)
DrdI 1052 GACAGACTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1529 CCATGG C^C[GA][CT]GG
EcoNI 1477 CCTTTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 128 CCCGC CCCGC(4/6)
FokI 1710 GGATG GGATG(9/13)
KpnI 20 GGTACC GGTAC^C
MaeII 59 ACGT A^CGT
MamI 1312 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1529 CCATGG C^CATGG
PflMI 48 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 259 ACCGGT A^CCGGT
PleI 1056 GACTC GAGTC(4/5)
PstI 455 CTGCAG CTGCA^G
RcaI 2024 TCATGA T^CATGA
SapI 1439 GCTCTTC GCTCTTC(1/4)
SgrAI 258 CACCGGTG C[GA]^CCGG[CT]G
SspBI 2277 TGTACA T^GTACA
Van91I 48 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwNI 1484 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1505 CAGGTTCTG
AspHI 788 GTGCTC G[AT]GC[AT]^C
835 GTGCTC
BalI 186 TGGCCA TGG^CCA
2004 TGGCCA
BglII 473 AGATCT A^GATCT
757 AGATCT
BlpI 27 GCTGAGC GC^T[ACGT]AGC
1000 GCTGAGC
Bpu1102I 27 GCTGAGC GC^T[ACGT]AGC
1000 GCTGAGC
BsgI 1682 CTGCAC GTGCAG(16/14)
1835 CTGCAC
BsiHKAI 788 GTGCTC G[AT]GC[AT]^C
835 GTGCTC
BsrDI 2097 CATTGC GCAATG(2/0)
1892 GCAATG
BsrFI 23 ACCGGC [GA]^CCGG[CT]
259 ACCGGT
CelII 27 GCTGAGC GC^T[ACGT]AGC
1000 GCTGAGC
CfoI 11 GCGC GCG^C
80 GCGC
Cfr10I 23 ACCGGC [GA]^CCGG[CT]
259 ACCGGT
DraII 65 GGGGCCT [GA]G^G[ACGT]CC[CT]
137 GGGCCCC
EarI 1440 CTCTTC CTCTTC(1/4)
1690 GAAGAG
EcoO109I 65 GGGGCCT [GA]G^G[ACGT]CC[CT]
137 GGGCCCC
EcoT14I 1276 CCTTGG C^C[AT][AT]GG
1529 CCATGG
EcoT22I 213 ATGCAT ATGCA^T
282 ATGCAT
HhaI 11 GCGC GCG^C
80 GCGC
HinP1I 11 GCGC G^CGC
80 GCGC
HpaI 1281 GTTAAC GTT^AAC
1701 GTTAAC
HphI 847 GGTGA GGTGA(8/7)
1132 GGTGA
Ksp632I 1440 CTCTTC CTCTTC(1/4)
1690 GAAGAG
MfeI 1217 CAATTG C^AATTG
1992 CAATTG
MluNI 186 TGGCCA TGG^CCA
2004 TGGCCA
MscI 186 TGGCCA TGG^CCA
2004 TGGCCA
MslI 1024 CATCTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1867 CACCCCAGTG
MunI 1217 CAATTG C^AATTG
1992 CAATTG
NsiI 213 ATGCAT ATGCA^T
282 ATGCAT
SfcI 455 CTGCAG C^T[GA][CT]AG
2361 CTGTAG
SmlI 225 CTCAAG C^T[CT][GA]AG
1726 CTTGAG
SspI 1594 AATATT AAT^ATT
1650 AATATT
StyI 1276 CCTTGG C^C[AT][AT]GG
1529 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BbvI BcnI BfaI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI HaeIII HapII
HincII HindII HinfI HpaII ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII