Restriction map for
pF1KB5109
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI Aor51HI ApoI AscI
Asp718I AspI AviII AvrII BamHI
BbrPI BcgI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaI BseRI BsiEI BspHI BspLU11I
BsrBI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MluI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 784 GACGTC GACGT^C
AccIII 223 TCCGGA T^CCGGA
AhdI 1280 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1357 GTGCAC G^TGCAC
ApaI 1467 GGGCCC GGGCC^C
ApaLI 1357 GTGCAC G^TGCAC
AseI 1036 ATTAAT AT^TAAT
AsnI 1036 ATTAAT AT^TAAT
AspEI 1280 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1249 CCCGGG C^[CT]CG[GA]G
BbeI 1501 GGCGCC GGCGC^C
BbvCI 243 GCTGAGG CCTCAGC(-5/-2)
BfaI 1456 CTAG C^TAG
BglI 474 GCCATACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 636 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 223 TCCGGA T^CCGGA
BsiWI 5 CGTACG C^GTACG
BsmI 514 GCATTC GAATGC(1/-1)
BsoBI 1249 CCCGGG C^[CT]CG[GA]G
Bsp1407I 98 TGTACA T^GTACA
BspDI 963 ATCGAT AT^CGAT
BspEI 223 TCCGGA T^CCGGA
BsrGI 98 TGTACA T^GTACA
BssHII 674 GCGCGC G^CGCGC
ClaI 963 ATCGAT AT^CGAT
DrdI 1396 GACGGCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1280 GACAGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 408 CTCTTC CTCTTC(1/4)
HaeII 1501 GGCGCC [GA]GCGC^[CT]
HincII 574 GTCAAC GT[CT]^[GA]AC
HindII 574 GTCAAC GT[CT]^[GA]AC
HphI 1811 TCACC GGTGA(8/7)
KasI 1501 GGCGCC G^GCGCC
Ksp632I 408 CTCTTC CTCTTC(1/4)
MaeI 1456 CTAG C^TAG
MamI 636 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1382 CAATTG C^AATTG
MroI 223 TCCGGA T^CCGGA
MseI 1037 TTAA T^TAA
MunI 1382 CAATTG C^AATTG
NaeI 470 GCCGGC GCC^GGC
NarI 1501 GGCGCC GG^CGCC
NcoI 818 CCATGG C^CATGG
NgoMIV 470 GCCGGC G^CCGGC
PpuMI 525 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 1036 ATTAAT AT^TAAT
PstI 1143 CTGCAG CTGCA^G
SapI 407 GCTCTTC GCTCTTC(1/4)
SexAI 1097 ACCAGGT A^CC[AT]GGT
SfoI 1501 GGCGCC GGC^GCC
SmaI 1249 CCCGGG CCC^GGG
SspBI 98 TGTACA T^GTACA
Tru9I 1037 TTAA T^TAA
XmaCI 1249 CCCGGG C^CCGGG
XmaI 1249 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 841 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
970 CAGTGTCTG
AocI 79 CCTAAGG CC^T[ACGT]AGG
123 CCTAAGG
Asp700I 18 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1308 GAAGTGCTTC
BalI 188 TGGCCA TGG^CCA
485 TGGCCA
BanI 1426 GGCACC G^G[CT][GA]CC
1501 GGCGCC
BciVI 255 GGATAC GTATCC(6/5)
1255 GGATAC
BsaWI 223 TCCGGA [AT]^CCGG[AT]
1645 TCCGGT
BsgI 937 CTGCAC GTGCAG(16/14)
1726 CTGCAC
BsmBI 54 CGTCTC CGTCTC(1/5)
1054 GAGACG
BspMI 872 ACCTGC ACCTGC(4/8)
39 GCAGGT
BsrDI 216 CATTGC GCAATG(2/0)
321 CATTGC
BstYI 248 GGATCT [GA]^GATC[CT]
1706 AGATCC
Bsu36I 79 CCTAAGG CC^T[ACGT]AGG
123 CCTAAGG
Eco81I 79 CCTAAGG CC^T[ACGT]AGG
123 CCTAAGG
EcoNI 33 CCTCCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1290 CCTGCTGAAGG
EcoT14I 818 CCATGG C^C[AT][AT]GG
1463 CCAAGG
MflI 248 GGATCT [GA]^GATC[CT]
1706 AGATCC
MluNI 188 TGGCCA TGG^CCA
485 TGGCCA
MscI 188 TGGCCA TGG^CCA
485 TGGCCA
PvuII 844 CAGCTG CAG^CTG
1140 CAGCTG
SfcI 1143 CTGCAG C^T[GA][CT]AG
1191 CTACAG
StyI 818 CCATGG C^C[AT][AT]GG
1463 CCAAGG
XcmI 294 CCAGCCTTTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1106 CCAGGATGACCGTGG
XhoII 248 GGATCT [GA]^GATC[CT]
1706 AGATCC
XmnI 18 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1308 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BanII BbsI
BbvI BcnI BmyI BpuAI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I