Restriction map for
pF1KB5110
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HindIII KasI KpnI Ksp632I KspI
MamI MfeI MroI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 89 ACGCGT A^C[GA][CT]GT
AocI 808 CCTGAGG CC^T[ACGT]AGG
Asp700I 1332 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 849 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 734 CCCGGG C^[CT]CG[GA]G
BalI 1604 TGGCCA TGG^CCA
BanI 1843 GGCACC G^G[CT][GA]CC
BclI 1038 TGATCA T^GATCA
BfaI 929 CTAG C^TAG
BpmI 1430 CTCCAG CTGGAG(16/14)
BsaAI 1526 CACGTA [CT]AC^GT[GA]
BsaWI 76 ACCGGA [AT]^CCGG[AT]
BsmAI 460 GAGAC GTCTC(1/5)
BsoBI 734 CCCGGG C^[CT]CG[GA]G
BspHI 1904 TCATGA T^CATGA
BsrFI 1846 ACCGGC [GA]^CCGG[CT]
Bsu36I 808 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1846 ACCGGC [GA]^CCGG[CT]
DraIII 947 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 808 CCTGAGG CC^T[ACGT]AGG
EcoNI 1902 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 32 GCGGG CCCGC(4/6)
FbaI 1038 TGATCA T^GATCA
HaeII 1247 AGCGCC [GA]GCGC^[CT]
HincII 85 GTTAAC GT[CT]^[GA]AC
HindII 85 GTTAAC GT[CT]^[GA]AC
HpaI 85 GTTAAC GTT^AAC
MaeI 929 CTAG C^TAG
MluI 89 ACGCGT A^CGCGT
MluNI 1604 TGGCCA TGG^CCA
MscI 1604 TGGCCA TGG^CCA
NdeI 230 CATATG CA^TATG
PflFI 849 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1716 CCATTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 588 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1904 TCATGA T^CATGA
ScaI 784 AGTACT AGT^ACT
SmaI 734 CCCGGG CCC^GGG
SphI 1571 GCATGC GCATG^C
Tth111I 849 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1716 CCATTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 734 CCCGGG C^CCGGG
XmaI 734 CCCGGG C^CCGGG
XmnI 1332 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccI 235 GTAGAC GT^[AC][GT]AC
1207 GTAGAC
AccII 90 CGCG CG^CG
640 CGCG
AcsI 412 AAATTT [GA]^AATT[CT]
1765 AAATTT
ApoI 412 AAATTT [GA]^AATT[CT]
1765 AAATTT
AspHI 40 GTGCTC G[AT]GC[AT]^C
392 GTGCTC
BbsI 1006 GAAGAC GAAGAC(2/6)
578 GTCTTC
BcgI 900 CGAAGGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1823 GCAAACCCATCG
BpuAI 1006 GAAGAC GAAGAC(2/6)
578 GTCTTC
BsgI 1180 GTGCAG GTGCAG(16/14)
1459 GTGCAG
BsiHKAI 40 GTGCTC G[AT]GC[AT]^C
392 GTGCTC
BstAPI 1066 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1182 GCAGATTCTGC
BstF5I 1230 GGATG GGATG(2/0)
1488 GGATG
BstUI 90 CGCG CG^CG
640 CGCG
BstXI 1100 CCAACAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1883 CCAAGGCTGTGG
CfoI 639 GCGC GCG^C
1248 GCGC
DraII 588 GGGTCCT [GA]G^G[ACGT]CC[CT]
974 AGGGCCT
DrdI 1069 GACATTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1681 GACCAGCTGGTC
EcoO109I 588 GGGTCCT [GA]G^G[ACGT]CC[CT]
974 AGGGCCT
FokI 1230 GGATG GGATG(9/13)
1488 GGATG
HgaI 1200 GCGTC GACGC(5/10)
1239 GCGTC
HhaI 639 GCGC GCG^C
1248 GCGC
HinP1I 639 GCGC G^CGC
1248 GCGC
MspA1I 1390 CAGCTG C[AC]G^C[GT]G
1684 CAGCTG
MvnI 90 CGCG CG^CG
640 CGCG
NcoI 1454 CCATGG C^CATGG
1505 CCATGG
PvuII 1390 CAGCTG CAG^CTG
1684 CAGCTG
SmlI 160 CTCAAG C^T[CT][GA]AG
996 CTTGAG
TaqI 5 TCGA T^CGA
843 TCGA
TthHB8I 5 TCGA T^CGA
843 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrDI BsrI BssKI BstNI BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I Fnu4HI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII