Restriction map for
pF1KB5120
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CfoI ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MaeII MamI MluI
MluNI MscI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 405 TCCGGA T^CCGGA
AfaI 660 GTAC GT^AC
Alw44I 1004 GTGCAC G^TGCAC
ApaLI 1004 GTGCAC G^TGCAC
BamHI 567 GGATCC G^GATCC
BanI 669 GGCACC G^G[CT][GA]CC
BanII 289 GAGCTC G[GA]GC[CT]^C
BbsI 910 GTCTTC GAAGAC(2/6)
BlpI 742 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 742 GCTGAGC GC^T[ACGT]AGC
BpuAI 910 GTCTTC GAAGAC(2/6)
BsaI 1208 GAGACC GGTCTC(1/5)
BseAI 405 TCCGGA T^CCGGA
BsmFI 1031 GGGAC GGGAC(10/14)
BspEI 405 TCCGGA T^CCGGA
BspHI 299 TCATGA T^CATGA
BspMI 1068 ACCTGC ACCTGC(4/8)
BsrFI 45 GCCGGT [GA]^CCGG[CT]
BstXI 516 CCATTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 567 GGATCC [GA]^GATC[CT]
CelII 742 GCTGAGC GC^T[ACGT]AGC
Cfr10I 45 GCCGGT [GA]^CCGG[CT]
DraI 871 TTTAAA TTT^AAA
DsaI 1212 CCGTGG C^C[GA][CT]GG
EarI 1075 CTCTTC CTCTTC(1/4)
EcoT14I 898 CCTTGG C^C[AT][AT]GG
HindIII 998 AAGCTT A^AGCTT
Ksp632I 1075 CTCTTC CTCTTC(1/4)
MfeI 938 CAATTG C^AATTG
MflI 567 GGATCC [GA]^GATC[CT]
MroI 405 TCCGGA T^CCGGA
MslI 174 CACTAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 938 CAATTG C^AATTG
PshAI 903 GACTAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 299 TCATGA T^CATGA
RsaI 660 GTAC GT^AC
SacI 289 GAGCTC GAGCT^C
SmlI 1091 CTCAAG C^T[CT][GA]AG
SspI 765 AATATT AAT^ATT
StyI 898 CCTTGG C^C[AT][AT]GG
SwaI 870 ATTTAAAT ATTT^AAAT
XhoII 567 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 415 GTATAC GT^[AC][GT]AC
966 GTATAC
AcsI 868 GAATTT [GA]^AATT[CT]
874 AAATTC
ApoI 868 GAATTT [GA]^AATT[CT]
874 AAATTC
AspHI 289 GAGCTC G[AT]GC[AT]^C
1004 GTGCAC
BsaWI 405 TCCGGA [AT]^CCGG[AT]
930 ACCGGA
BseRI 1136 CTCCTC GAGGAG(10/8)
626 GAGGAG
BsgI 1070 CTGCAC GTGCAG(16/14)
49 GTGCAG
BsiHKAI 289 GAGCTC G[AT]GC[AT]^C
1004 GTGCAC
Bst1107I 415 GTATAC GTA^TAC
966 GTATAC
BstF5I 895 CATCC GGATG(2/0)
1125 CATCC
BstZ17I 415 GTATAC GTA^TAC
966 GTATAC
Cac8I 41 GCAAGC GC[ACGT]^[ACGT]GC
727 GCTGGC
DdeI 743 CTGAG C^T[ACGT]AG
905 CTAAG
DraII 812 AGGTCCT [GA]G^G[ACGT]CC[CT]
1031 GGGACCC
DrdI 431 GACACTATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1033 GACCCAAATGTC
EcoO109I 812 AGGTCCT [GA]G^G[ACGT]CC[CT]
1031 GGGACCC
FokI 895 CATCC GGATG(9/13)
1125 CATCC
HaeIII 11 GGCC GG^CC
149 GGCC
HgaI 453 GACGC GACGC(5/10)
1189 GCGTC
PpuMI 812 AGGTCCT [GA]G^G[AT]CC[CT]
1031 GGGACCC
SfaNI 1195 GATGC GCATC(5/9)
1240 GCATC
SfcI 435 CTATAG C^T[GA][CT]AG
663 CTATAG
TaqI 285 TCGA T^CGA
441 TCGA
Tsp45I 109 GTGAC ^GT[GC]AC
596 GTGAC
TthHB8I 285 TCGA T^CGA
441 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Cfr13I DpnII EcoRII Fnu4HI
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI TfiI Tru9I TseI
Tsp509I TspRI