Restriction map for
pF1KB5159
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# NO CUT ENZYMES ( 152 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BsrI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
Eam1105I EarI Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HgaI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MflI MluI
MroI MseI MspA1I MunI NaeI
NarI NdeI NgoMIV NheI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp45I Tsp509I
TspRI Tth111I Van91I XbaI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 232 GACGTC GACGT^C
AcyI 232 GACGTC G[GA]^CG[CT]C
AfaI 99 GTAC GT^AC
Aor51HI 542 AGCGCT AGC^GCT
Asp700I 309 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 474 GTGCTC G[AT]GC[AT]^C
AvaII 297 GGTCC G^G[AT]CC
BalI 344 TGGCCA TGG^CCA
BcgI 471 CGAGTGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 274 CTGGAG CTGGAG(16/14)
BsaAI 100 TACGTG [CT]AC^GT[GA]
BsaHI 232 GACGTC G[GA]^CG[CT]C
BsiHKAI 474 GTGCTC G[AT]GC[AT]^C
BsmAI 514 GAGAC GTCTC(1/5)
BsmBI 514 GAGACG CGTCTC(1/5)
BsrBI 211 CCGCTC CCGCTC(-3/-3)
BssHII 525 GCGCGC G^CGCGC
BstXI 332 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 66 GATC ^GATC
DraII 296 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 437 CCATGG C^C[GA][CT]GG
Eco47III 542 AGCGCT AGC^GCT
EcoO109I 296 GGGTCCC [GA]G^G[ACGT]CC[CT]
Hin1I 232 GACGTC G[GA]^CG[CT]C
HinfI 268 GACTC G^A[ACGT]TC
HphI 306 GGTGA GGTGA(8/7)
MboI 66 GATC ^GATC
MluNI 344 TGGCCA TGG^CCA
MscI 344 TGGCCA TGG^CCA
NcoI 437 CCATGG C^CATGG
NdeII 66 GATC ^GATC
NotI 207 GCGGCCGC GC^GGCCGC
NspI 375 GCATGT [GA]CATG^[CT]
PaeR7I 469 CTCGAG C^TCGAG
PleI 268 GACTC GAGTC(4/5)
PpuMI 296 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 535 CTGCAG CTGCA^G
RsaI 99 GTAC GT^AC
Sau3AI 66 GATC ^GATC
SfcI 535 CTGCAG C^T[GA][CT]AG
SmlI 469 CTCGAG C^T[CT][GA]AG
XcmI 332 CCATCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 469 CTCGAG C^TCGAG
XmnI 309 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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BbsI 112 GTCTTC GAAGAC(2/6)
508 GTCTTC
BcnI 222 CCCGG CC^[GC]GG
264 CCCGG
BpuAI 112 GTCTTC GAAGAC(2/6)
508 GTCTTC
BsiYI 287 CCGAGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
432 CCGCGCCATGG
BslI 287 CCGAGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
432 CCGCGCCATGG
BspMI 359 ACCTGC ACCTGC(4/8)
153 GCAGGT
BstAPI 50 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
566 GCATCAACTGC
BstNI 186 CCAGG CC^[AT]GG
339 CCTGG
DrdI 226 GACACCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
370 GACGAGCATGTC
EagI 208 CGGCCG C^GGCCG
429 CGGCCG
EclXI 208 CGGCCG C^GGCCG
429 CGGCCG
Eco52I 208 CGGCCG C^GGCCG
429 CGGCCG
EcoRII 186 CCAGG ^CC[AT]GG
339 CCTGG
EcoT14I 437 CCATGG C^C[AT][AT]GG
488 CCAAGG
FauI 395 CCCGC CCCGC(4/6)
181 GCGGG
HaeII 165 GGCGCT [GA]GCGC^[CT]
542 AGCGCT
HapII 223 CCGG C^CGG
265 CCGG
HpaII 223 CCGG C^CGG
265 CCGG
MboII 113 TCTTC GAAGA(8/7)
509 TCTTC
MspI 223 CCGG C^CGG
265 CCGG
MvaI 186 CCAGG CC^[AT]GG
339 CCTGG
NciI 222 CCCGG CC^[GC]GG
264 CCCGG
SfaNI 449 GCATC GCATC(5/9)
566 GCATC
StyI 437 CCATGG C^C[AT][AT]GG
488 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI AvaI AviII
BbvI BmyI BsaJI BsiEI BsmFI
BsoBI Bsp1286I BssKI BstF5I BstUI
Cac8I CfoI Cfr13I EaeI Fnu4HI
FokI FspI HaeIII HhaI HinP1I
ItaI MaeII MnlI MslI MvnI
MwoI NlaIII NlaIV Sau96I ScrFI
TaqI TseI TthHB8I