Restriction map for
pF1KB5224
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BsaHI BsaI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstYI BstZ17I ClaI CpoI DpnII
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HindIII HpaI KasI
KpnI Ksp632I KspI MamI MboI
MfeI MflI MluI MunI NarI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 124 CGCG CG^CG
AccIII 386 TCCGGA T^CCGGA
AcsI 663 GAATTT [GA]^AATT[CT]
AocI 168 CCTCAGG CC^T[ACGT]AGG
ApoI 663 GAATTT [GA]^AATT[CT]
AspHI 63 GAGCAC G[AT]GC[AT]^C
BanI 401 GGCACC G^G[CT][GA]CC
BfaI 554 CTAG C^TAG
BglI 47 GCCGGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 701 GCTTAGC GC^T[ACGT]AGC
BmyI 63 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 701 GCTTAGC GC^T[ACGT]AGC
BsaAI 691 TACGTG [CT]AC^GT[GA]
BseAI 386 TCCGGA T^CCGGA
BsgI 3 GTGCAG GTGCAG(16/14)
BsiEI 490 CGGTCG CG[GA][CT]^CG
BsiHKAI 63 GAGCAC G[AT]GC[AT]^C
BsmAI 557 GAGAC GTCTC(1/5)
Bsp1286I 63 GAGCAC G[AGT]GC[ACT]^C
BspEI 386 TCCGGA T^CCGGA
BspHI 20 TCATGA T^CATGA
BssSI 262 CACGAG CACGAG(-5/-1)
BstAPI 534 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 124 CGCG CG^CG
Bsu36I 168 CCTCAGG CC^T[ACGT]AGG
CelII 701 GCTTAGC GC^T[ACGT]AGC
CfoI 125 GCGC GCG^C
DraII 512 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 142 GACTGTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 137 CCATGG C^C[GA][CT]GG
Eco81I 168 CCTCAGG CC^T[ACGT]AGG
EcoO109I 512 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 137 CCATGG C^C[AT][AT]GG
FauI 338 CCCGC CCCGC(4/6)
HgaI 250 GACGC GACGC(5/10)
HhaI 125 GCGC GCG^C
HinP1I 125 GCGC G^CGC
HincII 151 GTCAAC GT[CT]^[GA]AC
HindII 151 GTCAAC GT[CT]^[GA]AC
HphI 453 GGTGA GGTGA(8/7)
MaeI 554 CTAG C^TAG
MaeII 692 ACGT A^CGT
MaeIII 620 GTCAC ^GT[ACGT]AC
MroI 386 TCCGGA T^CCGGA
MseI 671 TTAA T^TAA
MvnI 124 CGCG CG^CG
NaeI 52 GCCGGC GCC^GGC
NcoI 137 CCATGG C^CATGG
NgoMIV 52 GCCGGC G^CCGGC
PflMI 132 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 111 GAGTC GAGTC(4/5)
PpuMI 512 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 535 CAGCTG CAG^CTG
RcaI 20 TCATGA T^CATGA
SspI 391 AATATT AAT^ATT
StuI 545 AGGCCT AGG^CCT
StyI 137 CCATGG C^C[AT][AT]GG
Tru9I 671 TTAA T^TAA
Tsp45I 620 GTCAC ^GT[GC]AC
Tsp509I 664 AATT ^AATT
Van91I 132 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 359 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
535 CAGCTGCTG
BbsI 376 GAAGAC GAAGAC(2/6)
573 GAAGAC
BcnI 48 CCGGG CC^[GC]GG
445 CCGGG
BpmI 57 CTGGAG CTGGAG(16/14)
529 CTGGAG
BpuAI 376 GAAGAC GAAGAC(2/6)
573 GAAGAC
BsaWI 386 TCCGGA [AT]^CCGG[AT]
503 ACCGGA
BsrFI 52 GCCGGC [GA]^CCGG[CT]
488 GCCGGT
BstXI 521 CCACTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
605 CCAAGAAGGTGG
Cfr10I 52 GCCGGC [GA]^CCGG[CT]
488 GCCGGT
EcoNI 405 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
689 CCTACGTGAGG
MspA1I 6 CAGCGG C[AC]G^C[GT]G
535 CAGCTG
NciI 48 CCGGG CC^[GC]GG
445 CCGGG
SfaNI 182 GCATC GCATC(5/9)
437 GCATC
TfiI 75 GATTC G^A[AT]TC
361 GATTC
XcmI 578 CCATGCTGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
707 CCAGAAATGGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AvaII BalI BbvI
BsaJI BseRI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EaeI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboII MluNI MnlI MscI
MslI MspI MvaI MwoI NlaIII
NlaIV PstI Sau96I ScrFI SfcI
TseI TspRI