Restriction map for
pF1KB5234
##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AocI ApoI AscI AseI AsnI
Asp700I AvrII BalI BamHI BbsI
BciVI BfrI BglI BlnI BpuAI
BsaI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspMI BsrBI BsrDI
BsrGI BsrI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EaeI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoT22I FseI HpaI MfeI
MluI MluNI MscI MunI NdeI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TspRI
XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 72 enzymes) #
###################################
Acc65I 860 GGTACC G^GTACC
AccI 465 GTCGAC GT^[AC][GT]AC
AccIII 704 TCCGGA T^CCGGA
AcyI 23 GGCGCC G[GA]^CG[CT]C
AflIII 1463 ACATGT A^C[GA][CT]GT
AhdI 1510 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 982 GTGCAC G^TGCAC
ApaLI 982 GTGCAC G^TGCAC
Asp718I 860 GGTACC G^GTACC
AspEI 1510 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 202 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 680 TGCGCA TGC^GCA
BbeI 23 GGCGCC GGCGC^C
BbrPI 232 CACGTG CAC^GTG
BbvCI 949 CCTCAGC CCTCAGC(-5/-2)
BcgI 272 CGAGATTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1226 TGATCA T^GATCA
BglII 557 AGATCT A^GATCT
BlpI 178 GCTGAGC GC^T[ACGT]AGC
BpmI 898 CTGGAG CTGGAG(16/14)
Bpu1102I 178 GCTGAGC GC^T[ACGT]AGC
BsaAI 232 CACGTG [CT]AC^GT[GA]
BsaBI 1389 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 23 GGCGCC G[GA]^CG[CT]C
BsaWI 704 TCCGGA [AT]^CCGG[AT]
BseAI 704 TCCGGA T^CCGGA
BsgI 349 GTGCAG GTGCAG(16/14)
BsmAI 383 GAGAC GTCTC(1/5)
BspEI 704 TCCGGA T^CCGGA
BspHI 521 TCATGA T^CATGA
BspLU11I 1463 ACATGT A^CATGT
CelII 178 GCTGAGC GC^T[ACGT]AGC
DraIII 1198 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1510 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 406 GAAGAG CTCTTC(1/4)
EcoNI 936 CCTTCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1453 GATATC GAT^ATC
FbaI 1226 TGATCA T^GATCA
FspI 680 TGCGCA TGC^GCA
Hin1I 23 GGCGCC G[GA]^CG[CT]C
HindIII 988 AAGCTT A^AGCTT
HphI 209 TCACC GGTGA(8/7)
KasI 23 GGCGCC G^GCGCC
KpnI 860 GGTACC GGTAC^C
Ksp632I 406 GAAGAG CTCTTC(1/4)
KspI 27 CCGCGG CCGC^GG
MamI 1389 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 704 TCCGGA T^CCGGA
MseI 1208 TTAA T^TAA
NaeI 87 GCCGGC GCC^GGC
NarI 23 GGCGCC GG^CGCC
NcoI 413 CCATGG C^CATGG
NgoMIV 87 GCCGGC G^CCGGC
NheI 1419 GCTAGC G^CTAGC
PaeR7I 380 CTCGAG C^TCGAG
PflFI 202 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 232 CACGTG CAC^GTG
PmlI 232 CACGTG CAC^GTG
RcaI 521 TCATGA T^CATGA
SacII 27 CCGCGG CCGC^GG
SalI 465 GTCGAC G^TCGAC
SapI 406 GAAGAGC GCTCTTC(1/4)
SexAI 1127 ACCAGGT A^CC[AT]GGT
SfoI 23 GGCGCC GGC^GCC
SmaI 644 CCCGGG CCC^GGG
SphI 693 GCATGC GCATG^C
Tru9I 1208 TTAA T^TAA
Tsp45I 208 GTCAC ^GT[GC]AC
Tth111I 202 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 380 CTCGAG C^TCGAG
XmaCI 644 CCCGGG C^CCGGG
XmaI 644 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwI 1023 GATCC GGATC(4/5)
1137 GGATC
Aor51HI 536 AGCGCT AGC^GCT
1061 AGCGCT
ApaI 287 GGGCCC GGGCC^C
602 GGGCCC
AvaII 1131 GGTCC G^G[AT]CC
1169 GGACC
BanI 23 GGCGCC G^G[CT][GA]CC
860 GGTACC
BsmFI 1168 GGGAC GGGAC(10/14)
443 GTCCC
BsrFI 87 GCCGGC [GA]^CCGG[CT]
1031 ACCGGC
BssSI 1357 CACGAG CACGAG(-5/-1)
508 CTCGTG
BstXI 890 CCATCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1565 CCAAGGAGATGG
BstYI 557 AGATCT [GA]^GATC[CT]
1022 AGATCC
Cfr10I 87 GCCGGC [GA]^CCGG[CT]
1031 ACCGGC
DsaI 27 CCGCGG C^C[GA][CT]GG
413 CCATGG
Eco47III 536 AGCGCT AGC^GCT
1061 AGCGCT
HgaI 727 GACGC GACGC(5/10)
463 GCGTC
HincII 138 GTCAAC GT[CT]^[GA]AC
465 GTCGAC
HindII 138 GTCAAC GT[CT]^[GA]AC
465 GTCGAC
MaeII 233 ACGT A^CGT
1202 ACGT
MaeIII 208 GTCAC ^GT[ACGT]AC
629 GTTAC
MflI 557 AGATCT [GA]^GATC[CT]
1022 AGATCC
MslI 516 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
996 CATCGTGGTG
NspI 693 GCATGC [GA]CATG^[CT]
1463 ACATGT
PflMI 96 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1128 CCAGGTCCTGG
PleI 1089 GACTC GAGTC(4/5)
1516 GAGTC
PpuMI 1130 AGGTCCT [GA]G^G[AT]CC[CT]
1168 GGGACCT
PvuII 895 CAGCTG CAG^CTG
959 CAGCTG
ScaI 586 AGTACT AGT^ACT
902 AGTACT
TaqI 381 TCGA T^CGA
466 TCGA
Tsp509I 769 AATT ^AATT
1182 AATT
TthHB8I 381 TCGA T^CGA
466 TCGA
Van91I 96 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1128 CCAGGTCCTGG
XhoII 557 AGATCT [GA]^GATC[CT]
1022 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI BanII BbvI BcnI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeI MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI TseI