Restriction map for
pF1KB5258
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FokI FseI FspI
HindIII HpaI HphI Ksp632I KspI
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
MvnI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SapI SbfI ScaI SexAI SfaNI
SfcI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tsp45I Tth111I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 839 GGTACC G^GTACC
AcsI 933 GAATTC [GA]^AATT[CT]
AhdI 810 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 927 AGCT AG^CT
AlwNI 377 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 741 GGGCCC GGGCC^C
ApoI 933 GAATTC [GA]^AATT[CT]
Asp718I 839 GGTACC G^GTACC
AspEI 810 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 527 GTGCTC G[AT]GC[AT]^C
BanII 741 GGGCCC G[GA]GC[CT]^C
BbeI 135 GGCGCC GGCGC^C
BbvCI 57 CCTCAGC CCTCAGC(-5/-2)
BfaI 764 CTAG C^TAG
BglI 113 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 952 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 952 GCTGAGC GC^T[ACGT]AGC
BseRI 746 CTCCTC GAGGAG(10/8)
BsiHKAI 527 GTGCTC G[AT]GC[AT]^C
BsmFI 149 GGGAC GGGAC(10/14)
Bsp1407I 255 TGTACA T^GTACA
BsrDI 776 GCAATG GCAATG(2/0)
BsrGI 255 TGTACA T^GTACA
CelII 952 GCTGAGC GC^T[ACGT]AGC
DrdI 151 GACAAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 810 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 560 CCTACCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 933 GAATTC G^AATTC
HincII 611 GTCGAC GT[CT]^[GA]AC
HindII 611 GTCGAC GT[CT]^[GA]AC
HinfI 731 GATTC G^A[ACGT]TC
KasI 135 GGCGCC G^GCGCC
KpnI 839 GGTACC GGTAC^C
MaeI 764 CTAG C^TAG
NarI 135 GGCGCC GG^CGCC
NlaIII 591 CATG CATG^
NspI 590 ACATGC [GA]CATG^[CT]
PflMI 889 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 926 CAGCTG CAG^CTG
SalI 611 GTCGAC G^TCGAC
SfiI 112 GGCCCGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 135 GGCGCC GGC^GCC
SmaI 114 CCCGGG CCC^GGG
SspBI 255 TGTACA T^GTACA
TaqI 612 TCGA T^CGA
TfiI 731 GATTC G^A[AT]TC
TthHB8I 612 TCGA T^CGA
Van91I 889 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 114 CCCGGG C^CCGGG
XmaI 114 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 611 GTCGAC GT^[AC][GT]AC
689 GTAGAC
AlwI 919 GATCC GGATC(4/5)
517 GGATC
AvaI 114 CCCGGG C^[CT]CG[GA]G
946 CCCGAG
AvaII 379 GGTCC G^G[AT]CC
457 GGTCC
BanI 135 GGCGCC G^G[CT][GA]CC
839 GGTACC
BmyI 527 GTGCTC G[AGT]GC[ACT]^C
741 GGGCCC
BsgI 637 GTGCAG GTGCAG(16/14)
674 GTGCAG
BsoBI 114 CCCGGG C^[CT]CG[GA]G
946 CCCGAG
Bsp1286I 527 GTGCTC G[AGT]GC[ACT]^C
741 GGGCCC
BsrBI 941 CCGCTC CCGCTC(-3/-3)
105 GAGCGG
BsrFI 142 GCCGGC [GA]^CCGG[CT]
554 GCCGGC
BsrI 394 CCAGT ACTGG(1/-1)
694 CCAGT
Cfr10I 142 GCCGGC [GA]^CCGG[CT]
554 GCCGGC
DpnII 518 GATC ^GATC
919 GATC
EcoT14I 239 CCAAGG C^C[AT][AT]GG
813 CCAAGG
HaeII 61 AGCGCC [GA]GCGC^[CT]
135 GGCGCC
MaeII 447 ACGT A^CGT
834 ACGT
MaeIII 373 GTTAC ^GT[ACGT]AC
425 GTAAC
MboI 518 GATC ^GATC
919 GATC
MspA1I 93 CAGCGG C[AC]G^C[GT]G
926 CAGCTG
NaeI 142 GCCGGC GCC^GGC
554 GCCGGC
NdeII 518 GATC ^GATC
919 GATC
NgoMIV 142 GCCGGC G^CCGGC
554 GCCGGC
PpuMI 378 AGGTCCT [GA]G^G[AT]CC[CT]
456 GGGTCCT
Sau3AI 518 GATC ^GATC
919 GATC
StyI 239 CCAAGG C^C[AT][AT]GG
813 CCAAGG
TspRI 303 CAGTG CA[GC]TG[ACGT][ACGT]^
695 CAGTG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AcyI AfaI BbvI BcnI
BsaHI BsaI BsaJI BsiYI BslI
BsmAI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
MboII MnlI MspI MvaI MwoI
NciI NlaIV RsaI Sau96I ScrFI
TseI Tsp509I