Restriction map for
pF1KB5263
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII AccIII AclI AcsI AflIII
AgeI Alw44I ApaLI ApoI AscI
AseI AsnI Asp700I AspI AvrII
BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfaI BglI BlnI
BpuAI BsaI BseAI BsiEI BsmBI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI EarI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI KasI Ksp632I KspI MaeI
MaeIII MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tsp45I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 67 enzymes) #
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Acc65I 626 GGTACC G^GTACC
AccI 352 GTCTAC GT^[AC][GT]AC
AcyI 324 GGCGTC G[GA]^CG[CT]C
AflII 538 CTTAAG C^TTAAG
AhdI 679 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1066 CCTAAGG CC^T[ACGT]AGG
Aor51HI 969 AGCGCT AGC^GCT
Asp718I 626 GGTACC G^GTACC
AspEI 679 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 188 TGCGCA TGC^GCA
BalI 203 TGGCCA TGG^CCA
BanI 626 GGTACC G^G[CT][GA]CC
BbrPI 184 CACGTG CAC^GTG
BfrI 538 CTTAAG C^TTAAG
BglII 495 AGATCT A^GATCT
BlpI 411 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 411 GCTGAGC GC^T[ACGT]AGC
BsaAI 184 CACGTG [CT]AC^GT[GA]
BsaBI 264 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 324 GGCGTC G[GA]^CG[CT]C
BsaWI 923 ACCGGA [AT]^CCGG[AT]
BseRI 1027 GAGGAG GAGGAG(10/8)
BsgI 361 GTGCAG GTGCAG(16/14)
BsiWI 155 CGTACG C^GTACG
BsmFI 70 GTCCC GGGAC(10/14)
BsmI 698 GCATTC GAATGC(1/-1)
Bsp1407I 386 TGTACA T^GTACA
BsrBI 1072 GAGCGG CCGCTC(-3/-3)
BsrDI 416 GCAATG GCAATG(2/0)
BsrGI 386 TGTACA T^GTACA
BsrI 1018 CCAGT ACTGG(1/-1)
BstBI 226 TTCGAA TT^CGAA
BstXI 1046 CCATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1066 CCTAAGG CC^T[ACGT]AGG
CelII 411 GCTGAGC GC^T[ACGT]AGC
DsaI 1046 CCATGG C^C[GA][CT]GG
Eam1105I 679 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 969 AGCGCT AGC^GCT
Eco81I 1066 CCTAAGG CC^T[ACGT]AGG
EcoNI 1062 CCTACCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 188 TGCGCA TGC^GCA
HaeII 969 AGCGCT [GA]GCGC^[CT]
HgaI 325 GCGTC GACGC(5/10)
Hin1I 324 GGCGTC G[GA]^CG[CT]C
HincII 930 GTTAAC GT[CT]^[GA]AC
HindII 930 GTTAAC GT[CT]^[GA]AC
HindIII 880 AAGCTT A^AGCTT
HpaI 930 GTTAAC GTT^AAC
KpnI 626 GGTACC GGTAC^C
MamI 264 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 203 TGGCCA TGG^CCA
MscI 203 TGGCCA TGG^CCA
MspA1I 409 CAGCTG C[AC]G^C[GT]G
NcoI 1046 CCATGG C^CATGG
NspV 226 TTCGAA TT^CGAA
PflMI 765 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 184 CACGTG CAC^GTG
PmlI 184 CACGTG CAC^GTG
PvuII 409 CAGCTG CAG^CTG
SacI 1084 GAGCTC GAGCT^C
ScaI 995 AGTACT AGT^ACT
SfcI 841 CTACAG C^T[GA][CT]AG
SfuI 226 TTCGAA TT^CGAA
SspBI 386 TGTACA T^GTACA
Tsp509I 808 AATT ^AATT
Van91I 765 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 857 CCAAGACCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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ApaI 114 GGGCCC GGGCC^C
632 GGGCCC
AspHI 484 GTGCTC G[AT]GC[AT]^C
1084 GAGCTC
AvaI 63 CCCGGG C^[CT]CG[GA]G
72 CCCGGG
BpmI 988 CTGGAG CTGGAG(16/14)
1126 CTGGAG
BsiHKAI 484 GTGCTC G[AT]GC[AT]^C
1084 GAGCTC
BsoBI 63 CCCGGG C^[CT]CG[GA]G
72 CCCGGG
BsrFI 592 ACCGGC [GA]^CCGG[CT]
722 ACCGGC
Cfr10I 592 ACCGGC [GA]^CCGG[CT]
722 ACCGGC
EaeI 203 TGGCCA [CT]^GGCC[GA]
1022 TGGCCG
HinfI 195 GACTC G^A[ACGT]TC
898 GACTC
MslI 289 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
816 CATCAACATG
PleI 195 GACTC GAGTC(4/5)
898 GACTC
SmaI 63 CCCGGG CCC^GGG
72 CCCGGG
SmlI 460 CTCAAG C^T[CT][GA]AG
538 CTTAAG
TspRI 959 CAGTG CA[GC]TG[ACGT][ACGT]^
1019 CAGTG
XmaCI 63 CCCGGG C^CCGGG
72 CCCGGG
XmaI 63 CCCGGG C^CCGGG
72 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BspMI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MboI MboII MflI
MnlI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI Tru9I TseI
TthHB8I XhoII