Restriction map for
pF1KB5302
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 494 GTCTAC GT^[AC][GT]AC
AfaI 908 GTAC GT^AC
AflII 307 CTTAAG C^TTAAG
AflIII 894 ACATGT A^C[GA][CT]GT
AlwNI 152 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 9 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 638 CTCGAG C^[CT]CG[GA]G
BanI 749 GGTGCC G^G[CT][GA]CC
BanII 716 GAGCTC G[GA]GC[CT]^C
BcgI 135 GCAAGAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 307 CTTAAG C^TTAAG
BsaI 505 GGTCTC GGTCTC(1/5)
BsaWI 758 ACCGGA [AT]^CCGG[AT]
BsmFI 75 GTCCC GGGAC(10/14)
BsoBI 638 CTCGAG C^[CT]CG[GA]G
BspLU11I 894 ACATGT A^CATGT
BssKI 78 CCAGG ^CC[ACGT]GG
BstEII 808 GGTAACC G^GT[ACGT]ACC
BstF5I 477 GGATG GGATG(2/0)
BstNI 78 CCAGG CC^[AT]GG
BstPI 808 GGTAACC G^GT[ACGT]ACC
BstXI 20 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 674 CTGAG C^T[ACGT]AG
DsaI 615 CCGTGG C^C[GA][CT]GG
EcoNI 877 CCTCGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 808 GGTAACC G^GT[ACGT]ACC
EcoRII 78 CCAGG ^CC[AT]GG
FokI 477 GGATG GGATG(9/13)
HaeIII 697 GGCC GG^CC
HapII 759 CCGG C^CGG
HincII 259 GTTGAC GT[CT]^[GA]AC
HindII 259 GTTGAC GT[CT]^[GA]AC
HpaII 759 CCGG C^CGG
MspI 759 CCGG C^CGG
MvaI 78 CCAGG CC^[AT]GG
NspI 894 ACATGT [GA]CATG^[CT]
PaeR7I 638 CTCGAG C^TCGAG
PleI 73 GAGTC GAGTC(4/5)
RsaI 908 GTAC GT^AC
SacI 716 GAGCTC GAGCT^C
ScrFI 78 CCAGG CC^[ACGT]GG
SfaNI 329 GCATC GCATC(5/9)
SfcI 518 CTATAG C^T[GA][CT]AG
SspI 431 AATATT AAT^ATT
Tsp45I 587 GTGAC ^GT[GC]AC
XbaI 712 TCTAGA T^CTAGA
XhoI 638 CTCGAG C^TCGAG
XmnI 9 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AvaII 30 GGACC G^G[AT]CC
577 GGTCC
BfaI 713 CTAG C^TAG
727 CTAG
BglII 441 AGATCT A^GATCT
485 AGATCT
BseRI 635 CTCCTC GAGGAG(10/8)
641 GAGGAG
BsmAI 676 GAGAC GTCTC(1/5)
506 GTCTC
BspHI 186 TCATGA T^CATGA
351 TCATGA
Cac8I 59 GCAAGC GC[ACGT]^[ACGT]GC
887 GCGAGC
Cfr13I 30 GGACC G^G[ACGT]CC
577 GGTCC
EcoT14I 20 CCAAGG C^C[AT][AT]GG
470 CCTTGG
MaeI 713 CTAG C^TAG
727 CTAG
MaeII 131 ACGT A^CGT
703 ACGT
MslI 688 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
702 CACGTTGGTG
MspA1I 46 CAGCTG C[AC]G^C[GT]G
64 CAGCTG
NlaIV 576 GGGTCC GG[ACGT]^[ACGT]CC
749 GGTGCC
PvuII 46 CAGCTG CAG^CTG
64 CAGCTG
RcaI 186 TCATGA T^CATGA
351 TCATGA
Sau96I 30 GGACC G^G[ACGT]CC
577 GGTCC
SmlI 307 CTTAAG C^T[CT][GA]AG
638 CTCGAG
StyI 20 CCAAGG C^C[AT][AT]GG
470 CCTTGG
TfiI 124 GAATC G^A[AT]TC
348 GAATC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI AlwI AspHI BbvI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI BstYI DpnII Fnu4HI HinfI
HphI ItaI MaeIII MboI MboII
MflI MnlI MseI NdeII NlaIII
Sau3AI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII