Restriction map for
pF1KB5324
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BfrI BglI BglII BlnI BsaBI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KspI
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 223 GGTACC G^GTACC
AcyI 687 GACGCC G[GA]^CG[CT]C
Asp700I 1056 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 223 GGTACC G^GTACC
BbsI 1056 GAAGAC GAAGAC(2/6)
BbvCI 742 GCTGAGG CCTCAGC(-5/-2)
BclI 902 TGATCA T^GATCA
BlpI 192 GCTGAGC GC^T[ACGT]AGC
BpmI 764 CTCCAG CTGGAG(16/14)
Bpu1102I 192 GCTGAGC GC^T[ACGT]AGC
BpuAI 1056 GAAGAC GAAGAC(2/6)
BsaAI 249 CACGTA [CT]AC^GT[GA]
BsaHI 687 GACGCC G[GA]^CG[CT]C
BspHI 338 TCATGA T^CATGA
BsrBI 508 GAGCGG CCGCTC(-3/-3)
BsrDI 326 GCAATG GCAATG(2/0)
BstXI 1106 CCATCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1331 AGATCC [GA]^GATC[CT]
CelII 192 GCTGAGC GC^T[ACGT]AGC
FbaI 902 TGATCA T^GATCA
HaeII 1340 AGCGCC [GA]GCGC^[CT]
Hin1I 687 GACGCC G[GA]^CG[CT]C
KpnI 223 GGTACC GGTAC^C
MflI 1331 AGATCC [GA]^GATC[CT]
PpuMI 60 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 805 CTGCAG CTGCA^G
RcaI 338 TCATGA T^CATGA
SexAI 497 ACCTGGT A^CC[AT]GGT
SmlI 166 CTCAAG C^T[CT][GA]AG
StuI 809 AGGCCT AGG^CCT
TaqI 1005 TCGA T^CGA
TfiI 342 GAATC G^A[AT]TC
TthHB8I 1005 TCGA T^CGA
XcmI 1020 CCAGAAGCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1331 AGATCC [GA]^GATC[CT]
XmnI 1056 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 612 GTGCAC G^TGCAC
1354 GTGCAC
AlwI 1332 GATCC GGATC(4/5)
86 GGATC
AlwNI 493 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
737 CAGGGGCTG
ApaI 470 GGGCCC GGGCC^C
791 GGGCCC
ApaLI 612 GTGCAC G^TGCAC
1354 GTGCAC
AvaI 925 CCCGAG C^[CT]CG[GA]G
1186 CCCGAG
BalI 320 TGGCCA TGG^CCA
1046 TGGCCA
BanI 48 GGCACC G^G[CT][GA]CC
223 GGTACC
BfaI 109 CTAG C^TAG
520 CTAG
BsaI 1189 GAGACC GGTCTC(1/5)
728 GGTCTC
BseRI 761 CTCCTC GAGGAG(10/8)
880 GAGGAG
BsmBI 685 GAGACG CGTCTC(1/5)
1317 GAGACG
BsoBI 925 CCCGAG C^[CT]CG[GA]G
1186 CCCGAG
EaeI 320 TGGCCA [CT]^GGCC[GA]
1046 TGGCCA
EcoT14I 578 CCATGG C^C[AT][AT]GG
851 CCATGG
FauI 416 CCCGC CCCGC(4/6)
1370 CCCGC
HgaI 687 GACGC GACGC(5/10)
1319 GACGC
HincII 154 GTCAAC GT[CT]^[GA]AC
1225 GTCAAC
HindII 154 GTCAAC GT[CT]^[GA]AC
1225 GTCAAC
MaeI 109 CTAG C^TAG
520 CTAG
MaeII 250 ACGT A^CGT
1223 ACGT
MluNI 320 TGGCCA TGG^CCA
1046 TGGCCA
MscI 320 TGGCCA TGG^CCA
1046 TGGCCA
NcoI 578 CCATGG C^CATGG
851 CCATGG
NheI 108 GCTAGC G^CTAGC
519 GCTAGC
PvuII 816 CAGCTG CAG^CTG
1042 CAGCTG
SacI 163 GAGCTC GAGCT^C
960 GAGCTC
SfaNI 909 GATGC GCATC(5/9)
629 GCATC
SfcI 805 CTGCAG C^T[GA][CT]AG
813 CTACAG
StyI 578 CCATGG C^C[AT][AT]GG
851 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
TseI Tsp45I TspRI