Restriction map for
pF1KB5339
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaI BseAI BsgI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssSI
Bst1107I BstEII BstPI BstXI BstYI
BstZ17I Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV FbaI FseI FspI HindIII
HpaI KspI MamI MfeI MflI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 231 GGTACC G^GTACC
AfaI 232 GTAC GT^AC
AflIII 366 ACATGT A^C[GA][CT]GT
AlwNI 1135 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1417 CCTGAGG CC^T[ACGT]AGG
Asp718I 231 GGTACC G^GTACC
AvaII 1032 GGTCC G^G[AT]CC
BalI 1122 TGGCCA TGG^CCA
BanII 632 GGGCTC G[GA]GC[CT]^C
BbeI 6 GGCGCC GGCGC^C
BlpI 323 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 323 GCTCAGC GC^T[ACGT]AGC
BsaWI 356 ACCGGA [AT]^CCGG[AT]
BsmBI 19 GAGACG CGTCTC(1/5)
BspLU11I 366 ACATGT A^CATGT
BspMI 463 GCAGGT ACCTGC(4/8)
BsrDI 1485 CATTGC GCAATG(2/0)
BssHII 75 GCGCGC G^CGCGC
BstAPI 577 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 505 TTCGAA TT^CGAA
Bsu36I 1417 CCTGAGG CC^T[ACGT]AGG
CelII 323 GCTCAGC GC^T[ACGT]AGC
DpnII 748 GATC ^GATC
DsaI 14 CCGTGG C^C[GA][CT]GG
EaeI 1122 TGGCCA [CT]^GGCC[GA]
Eco81I 1417 CCTGAGG CC^T[ACGT]AGG
EcoRI 427 GAATTC G^AATTC
EcoT14I 602 CCAAGG C^C[AT][AT]GG
EcoT22I 772 ATGCAT ATGCA^T
HaeII 6 GGCGCC [GA]GCGC^[CT]
HincII 475 GTTGAC GT[CT]^[GA]AC
HindII 475 GTTGAC GT[CT]^[GA]AC
KasI 6 GGCGCC G^GCGCC
KpnI 231 GGTACC GGTAC^C
MaeII 1095 ACGT A^CGT
MboI 748 GATC ^GATC
MluNI 1122 TGGCCA TGG^CCA
MscI 1122 TGGCCA TGG^CCA
MspA1I 1320 CAGCTG C[AC]G^C[GT]G
NarI 6 GGCGCC GG^CGCC
NdeII 748 GATC ^GATC
NheI 612 GCTAGC G^CTAGC
NsiI 772 ATGCAT ATGCA^T
NspI 366 ACATGT [GA]CATG^[CT]
NspV 505 TTCGAA TT^CGAA
PflMI 829 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1320 CAGCTG CAG^CTG
RsaI 232 GTAC GT^AC
Sau3AI 748 GATC ^GATC
SfoI 6 GGCGCC GGC^GCC
SfuI 505 TTCGAA TT^CGAA
SpeI 1264 ACTAGT A^CTAGT
StyI 602 CCAAGG C^C[AT][AT]GG
Van91I 829 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AspHI 321 GTGCTC G[AT]GC[AT]^C
352 GAGCAC
BanI 6 GGCGCC G^G[CT][GA]CC
231 GGTACC
BcnI 51 CCCGG CC^[GC]GG
81 CCCGG
BseRI 103 GAGGAG GAGGAG(10/8)
1311 GAGGAG
BsiHKAI 321 GTGCTC G[AT]GC[AT]^C
352 GAGCAC
BsmFI 302 GGGAC GGGAC(10/14)
1207 GTCCC
BsmI 765 GAATGC GAATGC(1/-1)
543 GCATTC
EarI 733 GAAGAG CTCTTC(1/4)
1366 GAAGAG
FauI 1495 CCCGC CCCGC(4/6)
1299 GCGGG
HgaI 21 GACGC GACGC(5/10)
1155 GACGC
Ksp632I 733 GAAGAG CTCTTC(1/4)
1366 GAAGAG
NciI 51 CCCGG CC^[GC]GG
81 CCCGG
PleI 191 GACTC GAGTC(4/5)
787 GACTC
PstI 461 CTGCAG CTGCA^G
1160 CTGCAG
TfiI 140 GAATC G^A[AT]TC
415 GAATC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
ApoI BbvI BfaI BmyI BsaHI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboII
MnlI MseI MslI MspI MvaI
MvnI MwoI NlaIII NlaIV Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I