Restriction map for
pF1KB5390
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# NO CUT ENZYMES ( 81 enzymes) #
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Acc65I AclI AcsI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BcgI BciVI
BfrI BlnI BlpI Bpu1102I BsiWI
BsmI BspDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoT22I
FseI HincII HindII HindIII HpaI
KpnI KspI MluI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XhoI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1867 GACGTC GACGT^C
AccI 1645 GTCTAC GT^[AC][GT]AC
AccIII 2469 TCCGGA T^CCGGA
AspI 2173 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 545 TGCGCA TGC^GCA
BamHI 1380 GGATCC G^GATCC
BbrPI 819 CACGTG CAC^GTG
BclI 683 TGATCA T^GATCA
BglI 1992 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1433 AGATCT A^GATCT
BsaBI 2337 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2469 TCCGGA T^CCGGA
BsgI 220 GTGCAG GTGCAG(16/14)
BsmBI 2595 CGTCTC CGTCTC(1/5)
Bsp1407I 2477 TGTACA T^GTACA
BspEI 2469 TCCGGA T^CCGGA
BspMI 1649 ACCTGC ACCTGC(4/8)
BsrGI 2477 TGTACA T^GTACA
BstAPI 815 GCAACACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2306 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2587 GACAACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2687 CGGCCG C^GGCCG
EclXI 2687 CGGCCG C^GGCCG
Eco52I 2687 CGGCCG C^GGCCG
EcoRV 1573 GATATC GAT^ATC
FbaI 683 TGATCA T^GATCA
FspI 545 TGCGCA TGC^GCA
MamI 2337 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1968 CAATTG C^AATTG
MroI 2469 TCCGGA T^CCGGA
MunI 1968 CAATTG C^AATTG
NcoI 1376 CCATGG C^CATGG
PflFI 2173 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 819 CACGTG CAC^GTG
PmlI 819 CACGTG CAC^GTG
SapI 2229 GCTCTTC GCTCTTC(1/4)
ScaI 875 AGTACT AGT^ACT
SmaI 1736 CCCGGG CCC^GGG
SphI 2519 GCATGC GCATG^C
SspBI 2477 TGTACA T^GTACA
Tth111I 2173 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 384 TCTAGA T^CTAGA
XcmI 346 CCAGCAGCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1736 CCCGGG C^CCGGG
XmaI 1736 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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ApaI 1876 GGGCCC GGGCC^C
1924 GGGCCC
AspHI 904 GAGCTC G[AT]GC[AT]^C
2227 GAGCTC
BbvCI 849 CCTCAGC CCTCAGC(-5/-2)
2152 GCTGAGG
BsaWI 1170 ACCGGA [AT]^CCGG[AT]
2469 TCCGGA
BsiHKAI 904 GAGCTC G[AT]GC[AT]^C
2227 GAGCTC
BspHI 740 TCATGA T^CATGA
827 TCATGA
BspLU11I 752 ACATGT A^CATGT
1008 ACATGT
BsrBI 993 CCGCTC CCGCTC(-3/-3)
559 GAGCGG
DsaI 1245 CCACGG C^C[GA][CT]GG
1376 CCATGG
EcoNI 303 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2250 CCTGGAGAAGG
MseI 1118 TTAA T^TAA
2756 TTAA
NaeI 163 GCCGGC GCC^GGC
2689 GCCGGC
NgoMIV 163 GCCGGC G^CCGGC
2689 GCCGGC
PflMI 1044 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2658 CCAGGACGTGG
PpuMI 1112 AGGACCT [GA]G^G[AT]CC[CT]
2420 GGGACCT
RcaI 740 TCATGA T^CATGA
827 TCATGA
SacI 904 GAGCTC GAGCT^C
2227 GAGCTC
SexAI 365 ACCTGGT A^CC[AT]GGT
962 ACCTGGT
StuI 99 AGGCCT AGG^CCT
950 AGGCCT
Tru9I 1118 TTAA T^TAA
2756 TTAA
Tsp509I 1046 AATT ^AATT
1969 AATT
Van91I 1044 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2658 CCAGGACGTGG
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII