Restriction map for
pF1KB5432
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccI AccIII AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HpaI KasI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 265 GGTACC G^GTACC
AccII 739 CGCG CG^CG
AclI 386 AACGTT AA^CGTT
Asp718I 265 GGTACC G^GTACC
BciVI 197 GTATCC GTATCC(6/5)
BsgI 1191 GTGCAG GTGCAG(16/14)
BsmFI 378 GGGAC GGGAC(10/14)
Bsp1407I 209 TGTACA T^GTACA
BsrFI 568 GCCGGT [GA]^CCGG[CT]
BsrGI 209 TGTACA T^GTACA
BstAPI 1788 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 739 CGCG CG^CG
CfoI 740 GCGC GCG^C
Cfr10I 568 GCCGGT [GA]^CCGG[CT]
DraI 295 TTTAAA TTT^AAA
DraIII 425 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 344 GAATTC G^AATTC
EcoRV 655 GATATC GAT^ATC
FauI 1205 CCCGC CCCGC(4/6)
HhaI 740 GCGC GCG^C
HinP1I 740 GCGC G^CGC
HincII 168 GTTGAC GT[CT]^[GA]AC
HindII 168 GTTGAC GT[CT]^[GA]AC
HindIII 1402 AAGCTT A^AGCTT
KpnI 265 GGTACC GGTAC^C
MspA1I 1297 CAGCTG C[AC]G^C[GT]G
MvnI 739 CGCG CG^CG
NcoI 525 CCATGG C^CATGG
PpuMI 680 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 386 AACGTT AA^CGTT
PstI 1447 CTGCAG CTGCA^G
PvuII 1297 CAGCTG CAG^CTG
SacI 1131 GAGCTC GAGCT^C
SapI 1003 GAAGAGC GCTCTTC(1/4)
SmlI 841 CTCAAG C^T[CT][GA]AG
SspBI 209 TGTACA T^GTACA
TaqI 237 TCGA T^CGA
TthHB8I 237 TCGA T^CGA
XbaI 348 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 316 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1789 CAGGAGCTG
AocI 684 CCTCAGG CC^T[ACGT]AGG
1083 CCTGAGG
BanI 265 GGTACC G^G[CT][GA]CC
1239 GGTGCC
BanII 1131 GAGCTC G[GA]GC[CT]^C
1554 GGGCTC
BglII 240 AGATCT A^GATCT
959 AGATCT
BsaBI 235 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1597 GATTACAATC
BsaI 748 GAGACC GGTCTC(1/5)
953 GGTCTC
BsiYI 360 CCAGTTTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
893 CCAAGACCCGG
BslI 360 CCAGTTTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
893 CCAAGACCCGG
BspMI 770 GCAGGT ACCTGC(4/8)
1371 GCAGGT
Bsu36I 684 CCTCAGG CC^T[ACGT]AGG
1083 CCTGAGG
DraII 680 AGGACCT [GA]G^G[ACGT]CC[CT]
1087 AGGCCCT
DsaI 408 CCACGG C^C[GA][CT]GG
525 CCATGG
Eco81I 684 CCTCAGG CC^T[ACGT]AGG
1083 CCTGAGG
EcoO109I 680 AGGACCT [GA]G^G[ACGT]CC[CT]
1087 AGGCCCT
MaeII 308 ACGT A^CGT
387 ACGT
MamI 235 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1597 GATTACAATC
MslI 526 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1633 CACGATAATG
SfcI 648 CTACAG C^T[GA][CT]AG
1447 CTGCAG
SspI 92 AATATT AAT^ATT
301 AATATT
StuI 1472 AGGCCT AGG^CCT
1748 AGGCCT
TfiI 148 GAATC G^A[AT]TC
703 GAATC
Tsp45I 1279 GTGAC ^GT[GC]AC
1424 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsmAI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp509I TspRI XhoII