Restriction map for
pF1KB5439
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DdeI DraI DraII DraIII
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 411 GCGG CCGC(-3/-1)
AflII 520 CTTAAG C^TTAAG
AlwI 340 GATCC GGATC(4/5)
AvaI 335 CCCGAG C^[CT]CG[GA]G
BalI 401 TGGCCA TGG^CCA
BbsI 60 GAAGAC GAAGAC(2/6)
BcnI 588 CCGGG CC^[GC]GG
BfrI 520 CTTAAG C^TTAAG
BglI 466 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 413 GGGCAC G[AGT]GC[ACT]^C
BpuAI 60 GAAGAC GAAGAC(2/6)
BsaI 457 GAGACC GGTCTC(1/5)
BsgI 239 GTGCAG GTGCAG(16/14)
BsmI 513 GAATGC GAATGC(1/-1)
BsoBI 335 CCCGAG C^[CT]CG[GA]G
Bsp1286I 413 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 545 TGTACA T^GTACA
BspHI 71 TCATGA T^CATGA
BsrGI 545 TGTACA T^GTACA
BstAPI 508 GCACGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 476 CCATCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 339 AGATCC [GA]^GATC[CT]
Cfr13I 590 GGGCC G^G[ACGT]CC
DpnII 340 GATC ^GATC
DrdI 160 GACAGGCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 401 TGGCCA [CT]^GGCC[GA]
FauI 411 GCGGG CCCGC(4/6)
HapII 588 CCGG C^CGG
HpaII 588 CCGG C^CGG
HphI 147 GGTGA GGTGA(8/7)
MaeII 482 ACGT A^CGT
MboI 340 GATC ^GATC
MflI 339 AGATCC [GA]^GATC[CT]
MluNI 401 TGGCCA TGG^CCA
MscI 401 TGGCCA TGG^CCA
MseI 521 TTAA T^TAA
MslI 477 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 588 CCGG C^CGG
NciI 588 CCGGG CC^[GC]GG
NdeII 340 GATC ^GATC
NlaIII 72 CATG CATG^
NlaIV 206 GGTTCC GG[ACGT]^[ACGT]CC
PleI 53 GAGTC GAGTC(4/5)
PstI 612 CTGCAG CTGCA^G
RcaI 71 TCATGA T^CATGA
Sau3AI 340 GATC ^GATC
Sau96I 590 GGGCC G^G[ACGT]CC
SmlI 520 CTTAAG C^T[CT][GA]AG
SspBI 545 TGTACA T^GTACA
TaqI 386 TCGA T^CGA
TfiI 46 GATTC G^A[AT]TC
Tru9I 521 TTAA T^TAA
TspRI 462 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 386 TCGA T^CGA
XcmI 140 CCAAGGAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 339 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 94 AAATTC [GA]^AATT[CT]
553 GAATTT
AfaI 108 GTAC GT^AC
546 GTAC
ApoI 94 AAATTC [GA]^AATT[CT]
553 GAATTT
BbvI 248 GCTGC GCAGC(8/12)
611 GCTGC
BpmI 50 CTGGAG CTGGAG(16/14)
303 CTGGAG
BsiYI 404 CCACAAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
588 CCGGGCCAAGG
BslI 404 CCACAAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
588 CCGGGCCAAGG
BsmAI 457 GAGAC GTCTC(1/5)
213 GTCTC
BsmFI 185 GGGAC GGGAC(10/14)
332 GTCCC
Cac8I 411 GCGGGC GC[ACGT]^[ACGT]GC
489 GCAGGC
Fnu4HI 248 GCTGC GC^[ACGT]GC
611 GCTGC
HinfI 46 GATTC G^A[ACGT]TC
53 GAGTC
ItaI 248 GCTGC GC^[ACGT]GC
611 GCTGC
MaeIII 169 GTCAC ^GT[ACGT]AC
268 GTGAC
MboII 12 GAAGA GAAGA(8/7)
60 GAAGA
RsaI 108 GTAC GT^AC
546 GTAC
StuI 419 AGGCCT AGG^CCT
596 AGGCCT
TseI 248 GCTGC G^C[AT]GC
611 GCTGC
Tsp45I 169 GTCAC ^GT[GC]AC
268 GTGAC
Tsp509I 95 AATT ^AATT
554 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BfaI BsaJI BsrI BssKI
BstF5I BstNI EcoRII EcoT14I FokI
HaeIII MaeI MnlI MvaI MwoI
ScrFI SfcI StyI