Restriction map for
pF1KB5455
##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BanII
BbrPI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I CelII ClaI
CpoI DraI Eam1105I Eco47III EcoNI
EcoRI EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MaeI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SbfI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I Van91I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccI 28 GTCGAC GT^[AC][GT]AC
AcyI 107 GGCGCC G[GA]^CG[CT]C
AocI 772 CCTGAGG CC^T[ACGT]AGG
AspI 469 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 680 TGGCCA TGG^CCA
BbeI 107 GGCGCC GGCGC^C
BbsI 88 GTCTTC GAAGAC(2/6)
BcgI 498 CGAGGGGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 76 GCCCCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 706 CTGGAG CTGGAG(16/14)
BpuAI 88 GTCTTC GAAGAC(2/6)
BsaHI 107 GGCGCC G[GA]^CG[CT]C
BsaI 373 GAGACC GGTCTC(1/5)
BsaWI 463 TCCGGT [AT]^CCGG[AT]
BsiEI 608 CGGCCG CG[GA][CT]^CG
BsmAI 373 GAGAC GTCTC(1/5)
BsrFI 55 GCCGGC [GA]^CCGG[CT]
BstAPI 300 GCACCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 474 GGTCACC G^GT[ACGT]ACC
BstPI 474 GGTCACC G^GT[ACGT]ACC
BstYI 572 AGATCC [GA]^GATC[CT]
Bsu36I 772 CCTGAGG CC^T[ACGT]AGG
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
DrdI 730 GACGGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 608 CGGCCG C^GGCCG
EclXI 608 CGGCCG C^GGCCG
Eco52I 608 CGGCCG C^GGCCG
Eco81I 772 CCTGAGG CC^T[ACGT]AGG
EcoO65I 474 GGTCACC G^GT[ACGT]ACC
EcoRV 10 GATATC GAT^ATC
EcoT14I 335 CCAAGG C^C[AT][AT]GG
HaeII 107 GGCGCC [GA]GCGC^[CT]
HgaI 528 GCGTC GACGC(5/10)
Hin1I 107 GGCGCC G[GA]^CG[CT]C
HincII 28 GTCGAC GT[CT]^[GA]AC
HindII 28 GTCGAC GT[CT]^[GA]AC
KasI 107 GGCGCC G^GCGCC
MflI 572 AGATCC [GA]^GATC[CT]
MluNI 680 TGGCCA TGG^CCA
MscI 680 TGGCCA TGG^CCA
MseI 635 TTAA T^TAA
MspA1I 765 CCGCTG C[AC]G^C[GT]G
NaeI 55 GCCGGC GCC^GGC
NarI 107 GGCGCC GG^CGCC
NgoMIV 55 GCCGGC G^CCGGC
NspI 44 GCATGT [GA]CATG^[CT]
PflFI 469 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 548 GGGACCT [GA]G^G[AT]CC[CT]
SalI 28 GTCGAC G^TCGAC
SapI 711 GAAGAGC GCTCTTC(1/4)
ScaI 1007 AGTACT AGT^ACT
SfcI 591 CTACAG C^T[GA][CT]AG
SfoI 107 GGCGCC GGC^GCC
SmaI 80 CCCGGG CCC^GGG
StyI 335 CCAAGG C^C[AT][AT]GG
TfiI 151 GATTC G^A[AT]TC
Tru9I 635 TTAA T^TAA
Tth111I 469 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 572 AGATCC [GA]^GATC[CT]
XmaCI 80 CCCGGG C^CCGGG
XmaI 80 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 214 GAGCAC G[AT]GC[AT]^C
298 GAGCAC
AvaI 80 CCCGGG C^[CT]CG[GA]G
292 CCCGAG
AvaII 534 GGACC G^G[AT]CC
549 GGACC
BsaBI 978 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1062 GATGTGGATC
BsiHKAI 214 GAGCAC G[AT]GC[AT]^C
298 GAGCAC
BsmFI 236 GGGAC GGGAC(10/14)
548 GGGAC
BsoBI 80 CCCGGG C^[CT]CG[GA]G
292 CCCGAG
BspHI 566 TCATGA T^CATGA
845 TCATGA
BsrBI 613 GAGCGG CCGCTC(-3/-3)
757 GAGCGG
BsrI 233 ACTGG ACTGG(1/-1)
445 ACTGG
DdeI 773 CTGAG C^T[ACGT]AG
998 CTGAG
DraII 320 AGGCCCC [GA]G^G[ACGT]CC[CT]
548 GGGACCT
DraIII 301 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
481 CACACTGTG
DsaI 605 CCACGG C^C[GA][CT]GG
683 CCACGG
EarI 781 CTCTTC CTCTTC(1/4)
711 GAAGAG
EcoO109I 320 AGGCCCC [GA]G^G[ACGT]CC[CT]
548 GGGACCT
FauI 64 GCGGG CCCGC(4/6)
615 GCGGG
Ksp632I 781 CTCTTC CTCTTC(1/4)
711 GAAGAG
MamI 978 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1062 GATGTGGATC
MslI 450 CATCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
906 CACCACCATG
RcaI 566 TCATGA T^CATGA
845 TCATGA
XcmI 521 CCATCCTGCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
695 CCAGCTCCTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BanI BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BsrDI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I