Restriction map for
pF1KB5522
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MscI MslI
MunI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 114 CGCG CG^CG
AccIII 968 TCCGGA T^CCGGA
AlwI 971 GGATC GGATC(4/5)
AlwNI 492 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 422 AGCGCT AGC^GCT
ApaI 4 GGGCCC GGGCC^C
AspHI 901 GTGCTC G[AT]GC[AT]^C
AspI 52 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 993 CCCGAG C^[CT]CG[GA]G
BanII 4 GGGCCC G[GA]GC[CT]^C
BglI 799 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 181 CTGGAG CTGGAG(16/14)
BsaWI 968 TCCGGA [AT]^CCGG[AT]
BseAI 968 TCCGGA T^CCGGA
BseRI 403 CTCCTC GAGGAG(10/8)
BsgI 615 CTGCAC GTGCAG(16/14)
BsiHKAI 901 GTGCTC G[AT]GC[AT]^C
BsoBI 993 CCCGAG C^[CT]CG[GA]G
BspEI 968 TCCGGA T^CCGGA
BspMI 302 ACCTGC ACCTGC(4/8)
BsrFI 1036 GCCGGC [GA]^CCGG[CT]
BsrI 345 CCAGT ACTGG(1/-1)
BstUI 114 CGCG CG^CG
Cfr10I 1036 GCCGGC [GA]^CCGG[CT]
DpnII 972 GATC ^GATC
DraII 3 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 1110 GACCATTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 620 CCACGG C^C[GA][CT]GG
Eco47III 422 AGCGCT AGC^GCT
EcoNI 677 CCTTGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1045 GAATTC G^AATTC
EcoRV 67 GATATC GAT^ATC
HphI 849 GGTGA GGTGA(8/7)
MaeIII 860 GTGAC ^GT[ACGT]AC
MboI 972 GATC ^GATC
MroI 968 TCCGGA T^CCGGA
MseI 134 TTAA T^TAA
MvnI 114 CGCG CG^CG
NaeI 1036 GCCGGC GCC^GGC
NdeII 972 GATC ^GATC
NgoMIV 1036 GCCGGC G^CCGGC
PflFI 52 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 663 CTGCAG CTGCA^G
PvuII 567 CAGCTG CAG^CTG
SapI 813 GCTCTTC GCTCTTC(1/4)
Sau3AI 972 GATC ^GATC
ScaI 881 AGTACT AGT^ACT
SfcI 663 CTGCAG C^T[GA][CT]AG
SmlI 763 CTCAAG C^T[CT][GA]AG
TfiI 668 GAATC G^A[AT]TC
Tru9I 134 TTAA T^TAA
Tsp45I 860 GTGAC ^GT[GC]AC
Tth111I 52 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 11 CCAGCGTCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BanI 807 GGCACC G^G[CT][GA]CC
913 GGCACC
BsaI 1108 GAGACC GGTCTC(1/5)
45 GGTCTC
BsmFI 1097 GGGAC GGGAC(10/14)
16 GTCCC
EcoT14I 677 CCTTGG C^C[AT][AT]GG
725 CCAAGG
HincII 58 GTCAAC GT[CT]^[GA]AC
1132 GTCAAC
HindII 58 GTCAAC GT[CT]^[GA]AC
1132 GTCAAC
MspA1I 19 CCGCTG C[AC]G^C[GT]G
567 CAGCTG
PleI 687 GACTC GAGTC(4/5)
1003 GACTC
SfaNI 951 GATGC GCATC(5/9)
551 GCATC
StyI 677 CCTTGG C^C[AT][AT]GG
725 CCAAGG
TspRI 346 CAGTG CA[GC]TG[ACGT][ACGT]^
555 CAGTG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BbvI BmyI BpuAI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrBI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI EarI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeII MboII MnlI
MspI MvaI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI TseI Tsp509I