Restriction map for
pF1KB5541
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BalI BbeI BciVI BlnI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssHII BstBI BstEII
BstPI ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FseI HaeII HindIII
HpaI KasI KpnI MluI MluNI
MroI MscI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I RcaI RsrII SalI ScaI
SfiI SfoI SfuI SgrAI SmaI
SpeI SspBI SspI SwaI Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflII 1321 CTTAAG C^TTAAG
AocI 559 CCTGAGG CC^T[ACGT]AGG
ApaI 952 GGGCCC GGGCC^C
AspI 411 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1974 TGCGCA TGC^GCA
BclI 998 TGATCA T^GATCA
BfaI 2503 CTAG C^TAG
BfrI 1321 CTTAAG C^TTAAG
BglII 2694 AGATCT A^GATCT
BlpI 2226 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2226 GCTGAGC GC^T[ACGT]AGC
BsaBI 720 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2570 CGATCG CG[GA][CT]^CG
BsmI 525 GAATGC GAATGC(1/-1)
BspLU11I 198 ACATGT A^CATGT
Bst1107I 246 GTATAC GTA^TAC
BstAPI 1479 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 246 GTATAC GTA^TAC
Bsu36I 559 CCTGAGG CC^T[ACGT]AGG
CelII 2226 GCTGAGC GC^T[ACGT]AGC
DraI 165 TTTAAA TTT^AAA
EaeI 1787 TGGCCG [CT]^GGCC[GA]
Eco81I 559 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1402 ATGCAT ATGCA^T
FbaI 998 TGATCA T^GATCA
FspI 1974 TGCGCA TGC^GCA
KspI 924 CCGCGG CCGC^GG
MaeI 2503 CTAG C^TAG
MamI 720 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1751 CAATTG C^AATTG
MunI 1751 CAATTG C^AATTG
NaeI 3 GCCGGC GCC^GGC
NgoMIV 3 GCCGGC G^CCGGC
NsiI 1402 ATGCAT ATGCA^T
PflFI 411 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 2570 CGATCG CGAT^CG
SacII 924 CCGCGG CCGC^GG
SapI 205 GAAGAGC GCTCTTC(1/4)
SbfI 183 CCTGCAGG CCTGCA^GG
SexAI 1214 ACCTGGT A^CC[AT]GGT
SnaBI 2296 TACGTA TAC^GTA
Sse8387I 183 CCTGCAGG CCTGCA^GG
StuI 273 AGGCCT AGG^CCT
TaqI 1925 TCGA T^CGA
Tth111I 411 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1925 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 246 GTATAC GT^[AC][GT]AC
1113 GTCTAC
AccII 925 CGCG CG^CG
1780 CGCG
AcsI 1703 AAATTT [GA]^AATT[CT]
2662 AAATTC
AflIII 198 ACATGT A^C[GA][CT]GT
1535 ACACGT
AlwNI 1459 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1480 CAGCAGCTG
ApoI 1703 AAATTT [GA]^AATT[CT]
2662 AAATTC
BbrPI 2414 CACGTG CAC^GTG
2473 CACGTG
BbsI 2233 GAAGAC GAAGAC(2/6)
2401 GAAGAC
BbvCI 2462 CCTCAGC CCTCAGC(-5/-2)
1741 GCTGAGG
BglI 3 GCCGGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1627 GCCTCCAAGGC
BpuAI 2233 GAAGAC GAAGAC(2/6)
2401 GAAGAC
BsaI 742 GAGACC GGTCTC(1/5)
2060 GAGACC
BsaWI 394 ACCGGA [AT]^CCGG[AT]
518 TCCGGT
BsmBI 2801 CGTCTC CGTCTC(1/5)
2758 GAGACG
BspMI 2654 ACCTGC ACCTGC(4/8)
186 GCAGGT
BsrDI 1917 CATTGC GCAATG(2/0)
2409 CATTGC
BsrFI 3 GCCGGC [GA]^CCGG[CT]
1901 ACCGGC
BstUI 925 CGCG CG^CG
1780 CGCG
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
1901 ACCGGC
DraIII 589 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
789 CACAAAGTG
HincII 105 GTCAAC GT[CT]^[GA]AC
1219 GTTGAC
HindII 105 GTCAAC GT[CT]^[GA]AC
1219 GTTGAC
MseI 166 TTAA T^TAA
1322 TTAA
MvnI 925 CGCG CG^CG
1780 CGCG
NcoI 1734 CCATGG C^CATGG
2840 CCATGG
PmaCI 2414 CACGTG CAC^GTG
2473 CACGTG
PmlI 2414 CACGTG CAC^GTG
2473 CACGTG
PvuII 1483 CAGCTG CAG^CTG
1840 CAGCTG
SmlI 1062 CTTGAG C^T[CT][GA]AG
1321 CTTAAG
SphI 126 GCATGC GCATG^C
1400 GCATGC
Tru9I 166 TTAA T^TAA
1322 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AatII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BamHI
BanI BanII BbvI BcgI BcnI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp45I Tsp509I TspRI XcmI
XhoII