Restriction map for
pF1KB5555
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BanI BbeI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BsaHI BsaI BseAI BsgI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 529 CGCG CG^CG
Alw44I 1051 GTGCAC G^TGCAC
AocI 579 CCTCAGG CC^T[ACGT]AGG
ApaLI 1051 GTGCAC G^TGCAC
AspHI 1051 GTGCAC G[AT]GC[AT]^C
AvaI 108 CTCGGG C^[CT]CG[GA]G
BalI 950 TGGCCA TGG^CCA
BamHI 223 GGATCC G^GATCC
BbrPI 1327 CACGTG CAC^GTG
BbsI 652 GAAGAC GAAGAC(2/6)
BcgI 96 CGACATGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1008 CCGGG CC^[GC]GG
BfaI 817 CTAG C^TAG
BlpI 1002 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1002 GCTGAGC GC^T[ACGT]AGC
BpuAI 652 GAAGAC GAAGAC(2/6)
BsaAI 1327 CACGTG [CT]AC^GT[GA]
BsaWI 237 TCCGGT [AT]^CCGG[AT]
BsiEI 1118 CGACCG CG[GA][CT]^CG
BsiHKAI 1051 GTGCAC G[AT]GC[AT]^C
BsiWI 65 CGTACG C^GTACG
BsmAI 234 GTCTC GTCTC(1/5)
BsoBI 108 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1133 TGTACA T^GTACA
BsrGI 1133 TGTACA T^GTACA
BstUI 529 CGCG CG^CG
BstXI 213 CCACATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 579 CCTCAGG CC^T[ACGT]AGG
CelII 1002 GCTGAGC GC^T[ACGT]AGC
DraII 1010 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 390 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1119 GACCGAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 950 TGGCCA [CT]^GGCC[GA]
EarI 677 GAAGAG CTCTTC(1/4)
Eco81I 579 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1010 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 702 GAATTC G^AATTC
HincII 190 GTCAAC GT[CT]^[GA]AC
HindII 190 GTCAAC GT[CT]^[GA]AC
Ksp632I 677 GAAGAG CTCTTC(1/4)
MaeI 817 CTAG C^TAG
MluNI 950 TGGCCA TGG^CCA
MscI 950 TGGCCA TGG^CCA
MseI 14 TTAA T^TAA
MvnI 529 CGCG CG^CG
NciI 1008 CCGGG CC^[GC]GG
PmaCI 1327 CACGTG CAC^GTG
PmlI 1327 CACGTG CAC^GTG
SfaNI 1255 GCATC GCATC(5/9)
SspBI 1133 TGTACA T^GTACA
TaqI 461 TCGA T^CGA
TfiI 1286 GAATC G^A[AT]TC
Tru9I 14 TTAA T^TAA
TthHB8I 461 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 702 GAATTC [GA]^AATT[CT]
1075 AAATTT
AhdI 40 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
804 GACCAGCAGTC
ApaI 798 GGGCCC GGGCC^C
1010 GGGCCC
ApoI 702 GAATTC [GA]^AATT[CT]
1075 AAATTT
AspEI 40 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
804 GACCAGCAGTC
AspI 667 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1119 GACCGAGTC
BanII 798 GGGCCC G[GA]GC[CT]^C
1010 GGGCCC
BsaBI 453 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
781 GATCCGTATC
BsrBI 71 GAGCGG CCGCTC(-3/-3)
967 GAGCGG
BsrDI 1092 CATTGC GCAATG(2/0)
417 GCAATG
DsaI 1086 CCACGG C^C[GA][CT]GG
1277 CCACGG
Eam1105I 40 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
804 GACCAGCAGTC
EcoT14I 89 CCAAGG C^C[AT][AT]GG
729 CCTTGG
HaeII 880 AGCGCC [GA]GCGC^[CT]
956 GGCGCT
HapII 238 CCGG C^CGG
1008 CCGG
HpaII 238 CCGG C^CGG
1008 CCGG
MamI 453 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
781 GATCCGTATC
MspA1I 844 CAGCTG C[AC]G^C[GT]G
891 CAGCTG
MspI 238 CCGG C^CGG
1008 CCGG
NspI 408 GCATGT [GA]CATG^[CT]
1160 ACATGC
PflFI 667 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1119 GACCGAGTC
PstI 246 CTGCAG CTGCA^G
1105 CTGCAG
PvuII 844 CAGCTG CAG^CTG
891 CAGCTG
SfcI 246 CTGCAG C^T[GA][CT]AG
1105 CTGCAG
StyI 89 CCAAGG C^C[AT][AT]GG
729 CCTTGG
Tsp45I 564 GTCAC ^GT[GC]AC
1228 GTCAC
Tth111I 667 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1119 GACCGAGTC
XcmI 157 CCACAGCAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
477 CCAGAACATGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV PflMI PleI RsaI
Sau3AI Sau96I ScrFI TseI Tsp509I
TspRI Van91I XhoII