Restriction map for
pF1KB5556
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AviII AvrII BalI
BamHI BanII BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FspI HindIII KpnI Ksp632I MaeI
MamI MboII MfeI MluI MluNI
MroI MscI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 505 GTCTAC GT^[AC][GT]AC
AhdI 736 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 736 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 558 GGCGCC GGCGC^C
BbvCI 516 CCTCAGC CCTCAGC(-5/-2)
BlpI 540 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 540 GCTGAGC GC^T[ACGT]AGC
BsiEI 575 CGGCCG CG[GA][CT]^CG
BsmAI 712 GAGAC GTCTC(1/5)
BspHI 935 TCATGA T^CATGA
BspMI 629 ACCTGC ACCTGC(4/8)
BstXI 731 CCATCGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 95 AGATCC [GA]^GATC[CT]
CelII 540 GCTGAGC GC^T[ACGT]AGC
DraII 668 AGGCCCT [GA]G^G[ACGT]CC[CT]
EagI 575 CGGCCG C^GGCCG
Eam1105I 736 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 575 CGGCCG C^GGCCG
Eco52I 575 CGGCCG C^GGCCG
EcoNI 672 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 668 AGGCCCT [GA]G^G[ACGT]CC[CT]
FseI 576 GGCCGGCC GGCCGG^CC
HpaI 946 GTTAAC GTT^AAC
KasI 558 GGCGCC G^GCGCC
MflI 95 AGATCC [GA]^GATC[CT]
MslI 732 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 577 GCCGGC GCC^GGC
NarI 558 GGCGCC GG^CGCC
NgoMIV 577 GCCGGC G^CCGGC
NspI 896 ACATGC [GA]CATG^[CT]
PleI 742 GAGTC GAGTC(4/5)
PstI 664 CTGCAG CTGCA^G
RcaI 935 TCATGA T^CATGA
SfiI 576 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 558 GGCGCC GGC^GCC
SmlI 205 CTCAAG C^T[CT][GA]AG
TaqI 734 TCGA T^CGA
TfiI 827 GAATC G^A[AT]TC
TthHB8I 734 TCGA T^CGA
XcmI 942 CCACGTTAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 95 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 96 GATCC GGATC(4/5)
755 GGATC
AlwNI 286 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
667 CAGGCCCTG
AvaI 220 CCCGAG C^[CT]CG[GA]G
697 CCCGAG
AvaII 70 GGACC G^G[AT]CC
174 GGACC
BanI 558 GGCGCC G^G[CT][GA]CC
956 GGTGCC
BcnI 706 CCCGG CC^[GC]GG
833 CCCGG
BglI 442 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
577 GCCGGCCTGGC
BseRI 178 CTCCTC GAGGAG(10/8)
679 GAGGAG
BsmFI 726 GTCCC GGGAC(10/14)
744 GTCCC
BsoBI 220 CCCGAG C^[CT]CG[GA]G
697 CCCGAG
BsrBI 66 GAGCGG CCGCTC(-3/-3)
751 GAGCGG
BssHII 345 GCGCGC G^CGCGC
536 GCGCGC
BstAPI 624 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
975 GCAGCAGTTGC
DpnII 96 GATC ^GATC
756 GATC
HincII 409 GTCAAC GT[CT]^[GA]AC
946 GTTAAC
HindII 409 GTCAAC GT[CT]^[GA]AC
946 GTTAAC
HinfI 742 GAGTC G^A[ACGT]TC
827 GAATC
HphI 182 TCACC GGTGA(8/7)
263 TCACC
KspI 434 CCGCGG CCGC^GG
658 CCGCGG
MboI 96 GATC ^GATC
756 GATC
MseI 923 TTAA T^TAA
947 TTAA
NciI 706 CCCGG CC^[GC]GG
833 CCCGG
NdeII 96 GATC ^GATC
756 GATC
SacII 434 CCGCGG CCGC^GG
658 CCGCGG
Sau3AI 96 GATC ^GATC
756 GATC
SfcI 81 CTACAG C^T[GA][CT]AG
664 CTGCAG
Tru9I 923 TTAA T^TAA
947 TTAA
Tsp45I 307 GTGAC ^GT[GC]AC
388 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AluI BbvI
BmyI BpmI BsaHI BsaJI BsgI
BsiYI BslI Bsp1286I BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DsaI EaeI
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI MaeII MaeIII MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIII NlaIV Sau96I ScrFI SfaNI
TseI TspRI