Restriction map for
pF1KB5571
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BlnI BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BssHII Bst1107I BstBI BstUI
BstZ17I CfoI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KspI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I StuI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1423 GGTACC G^GTACC
AccI 2343 GTCTAC GT^[AC][GT]AC
AlwNI 2636 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 223 CCTAAGG CC^T[ACGT]AGG
AseI 2155 ATTAAT AT^TAAT
AsnI 2155 ATTAAT AT^TAAT
Asp718I 1423 GGTACC G^GTACC
AspHI 2262 GAGCAC G[AT]GC[AT]^C
BanI 1423 GGTACC G^G[CT][GA]CC
BciVI 922 GGATAC GTATCC(6/5)
BclI 689 TGATCA T^GATCA
BlpI 1877 GCTTAGC GC^T[ACGT]AGC
BmyI 2262 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 1877 GCTTAGC GC^T[ACGT]AGC
BsaI 1931 GAGACC GGTCTC(1/5)
BsiHKAI 2262 GAGCAC G[AT]GC[AT]^C
Bsp1286I 2262 GAGCAC G[AGT]GC[ACT]^C
BsrBI 2371 GAGCGG CCGCTC(-3/-3)
BsrDI 1058 CATTGC GCAATG(2/0)
BssSI 1045 CTCGTG CACGAG(-5/-1)
BstEII 1997 GGTCACC G^GT[ACGT]ACC
BstPI 1997 GGTCACC G^GT[ACGT]ACC
BstXI 988 CCAAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 223 CCTAAGG CC^T[ACGT]AGG
CelII 1877 GCTTAGC GC^T[ACGT]AGC
DraI 477 TTTAAA TTT^AAA
DraII 1563 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 2282 CCGTGG C^C[GA][CT]GG
EaeI 2374 CGGCCA [CT]^GGCC[GA]
EarI 1898 CTCTTC CTCTTC(1/4)
Eco81I 223 CCTAAGG CC^T[ACGT]AGG
EcoO109I 1563 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1997 GGTCACC G^GT[ACGT]ACC
EcoRI 1813 GAATTC G^AATTC
EcoT22I 2196 ATGCAT ATGCA^T
FbaI 689 TGATCA T^GATCA
KpnI 1423 GGTACC GGTAC^C
Ksp632I 1898 CTCTTC CTCTTC(1/4)
MspA1I 1232 CAGCTG C[AC]G^C[GT]G
NsiI 2196 ATGCAT ATGCA^T
PpuMI 1563 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 2155 ATTAAT AT^TAAT
PvuII 1232 CAGCTG CAG^CTG
ScaI 2438 AGTACT AGT^ACT
SpeI 1747 ACTAGT A^CTAGT
SphI 2699 GCATGC GCATG^C
SspI 292 AATATT AAT^ATT
TaqI 2260 TCGA T^CGA
TthHB8I 2260 TCGA T^CGA
XbaI 72 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 13 enzymes) #
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AcsI 390 AAATTT [GA]^AATT[CT]
1813 GAATTC
ApoI 390 AAATTT [GA]^AATT[CT]
1813 GAATTC
BglII 2118 AGATCT A^GATCT
2382 AGATCT
BsaBI 1115 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1180 GATCGCTATC
BseRI 2009 CTCCTC GAGGAG(10/8)
2368 GAGGAG
BsmAI 463 GAGAC GTCTC(1/5)
1931 GAGAC
BsmFI 1616 GGGAC GGGAC(10/14)
2646 GGGAC
BstAPI 598 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2617 GCAGCAGATGC
EcoNI 609 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1555 CCTTCCAAAGG
HincII 3 GTTGAC GT[CT]^[GA]AC
574 GTCAAC
HindII 3 GTTGAC GT[CT]^[GA]AC
574 GTCAAC
MaeII 7 ACGT A^CGT
525 ACGT
MamI 1115 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1180 GATCGCTATC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbsI BbvI BfaI BpmI BpuAI
BsaJI BsiYI BslI BsmI Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII