Restriction map for
pF1KB5585
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HhaI HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeII MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 440 GGCGTC G[GA]^CG[CT]C
AflIII 821 ACATGT A^C[GA][CT]GT
AlwI 270 GGATC GGATC(4/5)
AlwNI 166 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 479 TGGCCA TGG^CCA
BbsI 292 GAAGAC GAAGAC(2/6)
BclI 359 TGATCA T^GATCA
BpuAI 292 GAAGAC GAAGAC(2/6)
BsaBI 102 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 440 GGCGTC G[GA]^CG[CT]C
BsmI 845 GCATTC GAATGC(1/-1)
BspLU11I 821 ACATGT A^CATGT
BssKI 155 CCAGG ^CC[ACGT]GG
BstAPI 760 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 155 CCAGG CC^[AT]GG
BstYI 270 GGATCT [GA]^GATC[CT]
DsaI 658 CCATGG C^C[GA][CT]GG
EaeI 479 TGGCCA [CT]^GGCC[GA]
EcoRI 28 GAATTC G^AATTC
EcoRII 155 CCAGG ^CC[AT]GG
FbaI 359 TGATCA T^GATCA
HgaI 441 GCGTC GACGC(5/10)
Hin1I 440 GGCGTC G[GA]^CG[CT]C
HphI 110 TCACC GGTGA(8/7)
MaeIII 443 GTCAC ^GT[ACGT]AC
MamI 102 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 270 GGATCT [GA]^GATC[CT]
MluNI 479 TGGCCA TGG^CCA
MscI 479 TGGCCA TGG^CCA
MslI 868 CATCAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 12 CAGCGG C[AC]G^C[GT]G
MvaI 155 CCAGG CC^[AT]GG
NcoI 658 CCATGG C^CATGG
NheI 562 GCTAGC G^CTAGC
NspI 821 ACATGT [GA]CATG^[CT]
PleI 594 GAGTC GAGTC(4/5)
PstI 758 CTGCAG CTGCA^G
ScrFI 155 CCAGG CC^[ACGT]GG
SfcI 758 CTGCAG C^T[GA][CT]AG
SpeI 261 ACTAGT A^CTAGT
TaqI 290 TCGA T^CGA
Tsp45I 443 GTCAC ^GT[GC]AC
TthHB8I 290 TCGA T^CGA
XhoII 270 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 843 CCGC CCGC(-3/-1)
14 GCGG
AvaII 280 GGACC G^G[AT]CC
857 GGACC
BsaJI 20 CCAAGG C^C[ACGT][ACGT]GG
658 CCATGG
BsiYI 221 CCCACATACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
658 CCATGGCAAGG
BslI 221 CCCACATACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
658 CCATGGCAAGG
BsmAI 404 GAGAC GTCTC(1/5)
494 GAGAC
BsmFI 86 GGGAC GGGAC(10/14)
279 GGGAC
BstF5I 160 CATCC GGATG(2/0)
216 CATCC
DdeI 146 CTCAG C^T[ACGT]AG
829 CTGAG
DraII 279 GGGACCT [GA]G^G[ACGT]CC[CT]
856 AGGACCC
EarI 298 CTCTTC CTCTTC(1/4)
930 CTCTTC
EcoO109I 279 GGGACCT [GA]G^G[ACGT]CC[CT]
856 AGGACCC
EcoT14I 20 CCAAGG C^C[AT][AT]GG
658 CCATGG
FokI 160 CATCC GGATG(9/13)
216 CATCC
Ksp632I 298 CTCTTC CTCTTC(1/4)
930 CTCTTC
PpuMI 279 GGGACCT [GA]G^G[AT]CC[CT]
856 AGGACCC
StyI 20 CCAAGG C^C[AT][AT]GG
658 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsrI Cac8I Cfr13I DpnII
Fnu4HI HaeIII HinfI ItaI MaeI
MboI MboII MnlI MseI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I SfaNI TfiI Tru9I TseI
Tsp509I TspRI